Method for detecting antifungal properties of nanometer inorganic material by ATP biofluorescence lgC-lgI standard curve method

Abstract

The invention relates to a method for detecting antifungal properties of a nanometer inorganic antibacterial material. The detection method comprises the detection steps of preparing and preprocessing samples; performing equipment selection, and preparing a reagent and a culture medium; preserving strains, performing activating, and preparing bacterium suspension; after methylthioninium chloride staining, counting the content C of viable bacteria in the bacterium suspension through a hemocytometer, and performing calibration on C of the inoculated bacterial liquid; establishing a standard curve of the lgC-relative fluorescence intensity log lgI ; performing sample inoculating and shaking culture; determining I of recovered liquid and reckoning the content C and T of viable bacteria; calculating antibacterial rate R or antibacterial activity value A; and performing result evaluation. The method has the characteristics that the anti-fungus properties, expressed by R or A, of the nanometer inorganic material is subjected to pinpoint quantitative test through an ATP fluorophotometer. The I of the recovered liquid of control samples and the I of the recovered liquid of antibacterial samples before and after shaking culture are determined according to the specification of the invention and are expressed with lgI, and R or A is calculated. A result assessment basis is provided. An ATP biofluorescence lgC-lgI standard curve method for detecting antifungal properties of the nanometer inorganic antibacterial material, which is researched and developed in the invention, can support improvement of product quality by an advanced detection technique.

Description

technical field [0001] The invention relates to a method for testing the antifungal properties of nano inorganic antibacterial materials, in particular to an ATP bioluminescent method for precise and quantitative detection of the antifungal properties of nano inorganic antibacterial materials characterized by antibacterial rate R or antibacterial activity value A using an ATP fluorescence photometer lgC B -lgI B The standard curve method belongs to the technical field of antibacterial function detection of nanometer inorganic materials. Background technique [0002] In recent years, with the rapid progress of social science and technology and the increasing awareness of people's health and environmental protection; nano-inorganic antibacterial materials are widely used in many fields such as building materials, home appliances, textiles, food bags, and daily chemicals, and their domestic market size has already exceeded 100 billion. And increasing year by year, but the lag...

AI Technical Summary

Problems solved by technology

In view of the current development trend of quantification, rapidity and simplification in the research field of foreign antibacterial material performance testing technology, the principle of ATP fluorescence analysis has been used to formulate ISO 20743:2007-2013 "Textiles-Determination of antibacterial activity of textile products (Textiles-Determination of antibacterial activity of textile products) Determination of Bacterial Performance)" and ISO 13629-1:2012 "Textiles-Determination of antifungal activity of textile products.Part1Luminescence (Determination of antifungal performance of textile antibacterial finishing textiles)", which stipulates that the antibacterial / mold performance can be characterized by the change of ATP content after sample inoculation Fluorescence measurement method, but it is only applicable to textile or microporous materials with water absorption and control sample fungus growth value > 0, and it is not suitable for nanometers with control sample fungal growth value < 0 in key technical aspects such as test effective conditions Grade powder inorganic antibacterial materials; at the same time, no evaluation of measurement uncertainty is provided

In addition, the relevant antibacterial performance characterization parameters of this standard method are relatively single, only involving the antibacterial activity value A, while the antibacterial rate R commonly used in my country

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine

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