A method for establishing performance evaluation system of c18 chromatographic column
A technology for establishing methods and chromatographic columns, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve problems such as the application of neural network models, and achieve the effect of accurately predicting the performance of chromatographic columns
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Embodiment 1
[0039] 1) Preparation of standard solution and test sample:
[0040] Hydrophobic detection mixed standard: Dissolve uracil, pentylbenzene, and butylbenzene in water, the concentration of uracil is 0.06 μg / mL, the concentration of pentylbenzene is 4 μg / mL, and the concentration of butylbenzene is 4 μg / mL, to obtain Hydrophobicity Assay Mixed Standard.
[0041] Stereoselective detection mixed standard: Dissolve uracil, triphenylene, and o-terphenyl in water, and the concentrations of uracil, triphenylene, and o-terphenyl are all 0.1 mg / mL to obtain a stereoselective detection mixed standard.
[0042] Total ion exchange capacity detection mixed standard: Dissolve uracil, phenol, and benzylamine in water, the concentration of uracil is 0.1mg / mL, and the concentration of phenol and benzylamine is 0.5mg / mL to obtain the total ion exchange capacity detection Mixed standards.
[0043] Mixed standards for acidic ion exchange capacity detection: Dissolve uracil, phenol, and benzylamin...
Embodiment 2
[0083] 1) Preparation of standard solution and test sample:
[0084] Hydrophobic detection mixed standard: dissolve uracil, pentylbenzene, and butylbenzene in methanol, the concentration of uracil is 0.08 μg / mL, the concentration of pentylbenzene is 6 μg / mL, and the concentration of butylbenzene is 6 μg / mL. Obtain the Hydrophobicity Assay Mixed Standard.
[0085] Stereoselective detection mixed standard: Dissolve uracil, triphenylene, and o-terphenyl in methanol, and the concentrations of uracil, triphenylene, and o-terphenyl are all 0.3mg / mL to obtain a stereoselective detection mixed standard .
[0086] Total ion exchange capacity detection mixed standard: Dissolve uracil, phenol, and benzylamine in methanol, the concentration of uracil is 0.3mg / mL, and the concentration of phenol and benzylamine is 0.8mg / mL to obtain the total ion exchange capacity Assay Mixed Standards.
[0087] Acidic ion exchange capacity detection mixed standard: dissolve uracil, phenol, and benzylam...
Embodiment 3
[0127] 1) Preparation of standard solution and test sample:
[0128] Hydrophobic detection mixed standard: dissolve uracil, pentylbenzene, and butylbenzene in ethanol, the concentration of uracil is 0.03 μg / mL, the concentration of pentylbenzene is 3 μg / mL, and the concentration of butylbenzene is 3 μg / mL. Obtain the Hydrophobicity Assay Mixed Standard.
[0129] Stereoselective detection mixed standard: Dissolve uracil, triphenylene, and o-terphenyl in ethanol, and the concentrations of uracil, triphenylene, and o-terphenyl are all 0.3mg / mL to obtain a stereoselective detection mixed standard .
[0130] Total ion exchange capacity detection mixed standard: Dissolve uracil, phenol, and benzylamine in ethanol, the concentration of uracil is 0.3mg / mL, and the concentration of phenol and benzylamine is 0.3mg / mL to obtain the total ion exchange capacity Assay Mixed Standards.
[0131] Acidic ion exchange capacity detection mixed standard: Dissolve uracil, phenol, and benzylamine...
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