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A method for establishing performance evaluation system of c18 chromatographic column

A technology for establishing methods and chromatographic columns, which is applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve problems such as the application of neural network models, and achieve the effect of accurately predicting the performance of chromatographic columns

Active Publication Date: 2021-11-30
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing chromatographic column evaluation methods, there is no relevant scheme to apply the neural network model to the relevant evaluation of chromatographic column performance

Method used

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  • A method for establishing performance evaluation system of c18 chromatographic column
  • A method for establishing performance evaluation system of c18 chromatographic column
  • A method for establishing performance evaluation system of c18 chromatographic column

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1) Preparation of standard solution and test sample:

[0040] Hydrophobic detection mixed standard: Dissolve uracil, pentylbenzene, and butylbenzene in water, the concentration of uracil is 0.06 μg / mL, the concentration of pentylbenzene is 4 μg / mL, and the concentration of butylbenzene is 4 μg / mL, to obtain Hydrophobicity Assay Mixed Standard.

[0041] Stereoselective detection mixed standard: Dissolve uracil, triphenylene, and o-terphenyl in water, and the concentrations of uracil, triphenylene, and o-terphenyl are all 0.1 mg / mL to obtain a stereoselective detection mixed standard.

[0042] Total ion exchange capacity detection mixed standard: Dissolve uracil, phenol, and benzylamine in water, the concentration of uracil is 0.1mg / mL, and the concentration of phenol and benzylamine is 0.5mg / mL to obtain the total ion exchange capacity detection Mixed standards.

[0043] Mixed standards for acidic ion exchange capacity detection: Dissolve uracil, phenol, and benzylamin...

Embodiment 2

[0083] 1) Preparation of standard solution and test sample:

[0084] Hydrophobic detection mixed standard: dissolve uracil, pentylbenzene, and butylbenzene in methanol, the concentration of uracil is 0.08 μg / mL, the concentration of pentylbenzene is 6 μg / mL, and the concentration of butylbenzene is 6 μg / mL. Obtain the Hydrophobicity Assay Mixed Standard.

[0085] Stereoselective detection mixed standard: Dissolve uracil, triphenylene, and o-terphenyl in methanol, and the concentrations of uracil, triphenylene, and o-terphenyl are all 0.3mg / mL to obtain a stereoselective detection mixed standard .

[0086] Total ion exchange capacity detection mixed standard: Dissolve uracil, phenol, and benzylamine in methanol, the concentration of uracil is 0.3mg / mL, and the concentration of phenol and benzylamine is 0.8mg / mL to obtain the total ion exchange capacity Assay Mixed Standards.

[0087] Acidic ion exchange capacity detection mixed standard: dissolve uracil, phenol, and benzylam...

Embodiment 3

[0127] 1) Preparation of standard solution and test sample:

[0128] Hydrophobic detection mixed standard: dissolve uracil, pentylbenzene, and butylbenzene in ethanol, the concentration of uracil is 0.03 μg / mL, the concentration of pentylbenzene is 3 μg / mL, and the concentration of butylbenzene is 3 μg / mL. Obtain the Hydrophobicity Assay Mixed Standard.

[0129] Stereoselective detection mixed standard: Dissolve uracil, triphenylene, and o-terphenyl in ethanol, and the concentrations of uracil, triphenylene, and o-terphenyl are all 0.3mg / mL to obtain a stereoselective detection mixed standard .

[0130] Total ion exchange capacity detection mixed standard: Dissolve uracil, phenol, and benzylamine in ethanol, the concentration of uracil is 0.3mg / mL, and the concentration of phenol and benzylamine is 0.3mg / mL to obtain the total ion exchange capacity Assay Mixed Standards.

[0131] Acidic ion exchange capacity detection mixed standard: Dissolve uracil, phenol, and benzylamine...

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Abstract

The invention discloses a method for establishing a chromatographic column performance evaluation system. The chromatographic performance parameters are characterized by the hydrophobicity, stereoselectivity, total ion exchange capacity, acidic ion exchange capacity and metal residue of the chromatographic column, combined with a multi-layer feedforward neural network model Cross-column prediction of tailing factor, chromatographic column efficiency, and separation of phenolic acids is systematic, comprehensive, fast, accurate, and simple.

Description

technical field [0001] The invention belongs to the technical field of chromatography, and in particular relates to a method for establishing a performance evaluation system of a C18 chromatographic column. Background technique [0002] The performance of the chromatographic column is the embodiment of the chromatographic separation ability. However, the relationship between the performance of the chromatographic column and the stationary phase of the chromatographic column that plays the main role in separation is difficult to establish a mathematical model with the traditional method. To represent. There are no widely accepted and universal test procedures for the classification and characterization of LC columns. [0003] Neural network is a mathematical algorithm model that can process information in a distributed manner. This kind of network depends on the complexity of the system. By adjusting the relationship between a large number of internal nodes and mastering the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/74G01N30/88
CPCG01N30/02G01N30/74G01N30/88G01N2030/027G01N2030/889
Inventor 乔斌李明珠那平
Owner TIANJIN UNIV
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