Polygonatum multiflorum seed germination method
A technology of Polygonatum polyflora, seeds, applied in the directions of seed and rhizome treatment, germination equipment, application, etc., can solve the problems of large base, unfavorable large-scale production, difficult to obtain a large number of seedlings, etc.
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Embodiment 1
[0030] 20 seeds were randomly selected, 4 parts in total, dried at 80°C for 48 hours, and the moisture content of the seeds was calculated; The first type as Figure 5 As shown in middle a, the seeds are yellow, shiny and transparent; the second type is as Figure 5 As shown in middle b, the seeds are light green and dull; the third type is as Figure 5 As shown in middle c, the seeds are dark green, with white star-shaped spots on the surface, dull. Subsequent observation found that the surface of the seed coat of the third type of seed was covered with a layer of pulp, so it was dark green.
[0031] Randomly select three types of Polygonatum multiflora seeds each 10, measure the length, width and thickness of seeds with a vernier caliper; Randomly select three types of Polygonatum multiflora seeds each 250, totally 4 parts, after weighing with an electronic balance, Calculate the thousand-grain weight of seeds.
[0032] Water content (%)=(a1-a2) / (a1-a0)×100%
[0033] In...
Embodiment 2
[0042] Take 50 whole seeds, half seeds and 50 seeds with micropylar-end testa, disinfect with diluted 84 disinfectant (1:50) for 10 minutes, place them in a 12cm petri dish with 2 layers of filter paper, add 10ml of deionized water was used to germinate in constant temperature incubators at 15, 20, 25, and 30°C, and the germination situation was counted every 24 hours, and water was added appropriately. Each treatment was repeated 3 times. Half a seed is to use a blade to cut off half of the endosperm along the hypocotyl of the seed, leaving half a seed with a complete embryo; to remove the micropylar end of the seed coat is to wipe off the micropylar end of the Polygonatum seed with sandpaper seed coat.
[0043] The germination period of Polygonatum multiflora seeds was set at 60 days, and the germination situation of the seeds was observed every day. The lower hypocotyl broke through the seed coat by 2 mm as germination, and the germination potential and germination rate of ...
Embodiment 3
[0052] Take half a seed and 50 seeds each with the micropyle-end testa removed, and set three temperature changes of 4°C / 25°C, 15°C / 25°C and 15°C / 30°C, all of which are 10h dark / 14h light alternating light conditions, set Repeat 4 times, germinate for 60 days under optimum temperature conditions, measure the germination rate of seeds, and the test results are as follows: figure 1 shown. Depend on figure 1 It can be seen that for half seeds, there is a significant difference between the 15°C (10h) / 25°C (14h) variable temperature treatment and the other two treatments; There was a significant difference between the 30°C (14h) variable temperature treatment and the 15°C (10h) / 25°C (14h) treatment, but there was no significant difference between the 4°C (10h) / 25°C (14h) treatment (p>0.05 ). Under constant temperature conditions of 25°C, the germination rates of half seeds and seeds with the micropyle removed were 57.33% and 64.67%, respectively. Germination rate, and there is ...
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