Somatostatin (SS) anti-idiotype monoclonal antibody vaccine and preparation method thereof

A technology of monoclonal antibody and somatostatin, which is applied in the fields of bioengineering and vaccine preparation, can solve the problems of time-consuming, labor-intensive and costly, and achieve the effect of promoting animal growth, promoting release, and high efficiency

Inactive Publication Date: 2019-09-27
西安德轩驰生物科技有限公司 +1
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  • Description
  • Claims
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Problems solved by technology

This method can promote the growth of animals, but it also requires multiple doses of large doses to produce good results, which is time-consuming, laborious and costly

Method used

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  • Somatostatin (SS) anti-idiotype monoclonal antibody vaccine and preparation method thereof
  • Somatostatin (SS) anti-idiotype monoclonal antibody vaccine and preparation method thereof
  • Somatostatin (SS) anti-idiotype monoclonal antibody vaccine and preparation method thereof

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preparation example Construction

[0023] The invention provides a method for preparing a somatostatin anti-idiotypic monoclonal antibody, comprising the following steps:

[0024] (1) Coupling somatostatin with a macromolecular protein carrier to obtain a somatostatin immunogen complex;

[0025] (2) Immunizing animals once with the somatostatin immunogen complex to obtain somatostatin antibodies;

[0026] (3) Using the somatostatin antibody to immunize the animals same or different from step (2) for a second time to obtain somatostatin anti-idiotype monoclonal antibody.

[0027] In the invention, the somatostatin is coupled with the macromolecule protein carrier to obtain the somatostatin immunogen complex. Since somatostatin is a small molecular peptide substance with low immunogenicity, the present invention firstly couples it to a macromolecular protein carrier to prepare the SS immunogen complex. The present invention does not specifically limit the source of the somatostatin (also known as somatostatin, ...

Embodiment 1

[0034] (1) Preparation of somatostatin immunogen complex

[0035] The standard product of somatostatin was purchased from Sigma Company of the United States, which was 14 amino acid somatostatin. Since somatostatin is a small molecular peptide substance with low immunogenicity, to prepare an antibody, it is first coupled to a macromolecular protein carrier to form a SS immunogen complex. The coupling method is: dissolve 10 mg of bovine serum albumin in 1 mL of PBS (pH: 6.5-7.0), dissolve 1 mg of somatostatin in 1 mL of distilled water, mix and shake well, then add 1 mL of 0.25 % glutaraldehyde solution, continue to stir for 5-10 minutes after the dropwise addition, and then leave it at room temperature to continue the reaction for 2-3 hours to prepare the SS immunogen complex. Aliquot and store in -20°C refrigerator for later use.

[0036] (2) Somatostatin antibody preparation

[0037] ①Immunity:

[0038] Mix the same volume of SS immunogen complex and incomplete Freund's ad...

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Abstract

The invention provides a preparation method of an SS anti-idiotype monoclonal antibody, and belongs to the technical field of bioengineering and vaccine preparation. The method disclosed by the invention comprises the following steps: (1) coupling SS with a macromolecular protein carrier to obtain an SS immunogen complex; (2) carrying out primary immunization on animals with the SS immunogen complex to obtain an SS antibody; (3) carrying out secondary immunization on the animals which are the same or different from the animals in the step (2) with the SS antibody to obtain an SS anti-idiotype monoclonal antibody. The SS anti-idiotypic monoclonal antibody can be prepared by using the method disclosed by the invention. The SS anti-idiotypic monoclonal antibody prepared by the invention can be used as the vaccine to immunize the animals, so that the animals can produce the SS antibodies to immunize and neutralize SS in vivo, thereby promoting the release of growth hormones to promote the growth of the animals.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and vaccine preparation, and in particular relates to a somatostatin anti-idiotype monoclonal antibody vaccine and a preparation method thereof. Background technique [0002] The growth of animals is mainly regulated by the hypothalamus-pituitary axis. The hypothalamus produces two important hormones, growth hormone-releasing hormone (GHRH) and somatostatin (SS). animal growth. Among them, somatotropin-releasing hormone promotes the release of growth hormone from the pituitary gland, thereby promoting the growth of animals. Somatostatin (also known as growth hormone-releasing inhibitory hormone) inhibits the release of growth hormone from the pituitary gland, thereby inhibiting the growth of animals. [0003] According to the principle of animal growth regulation, people have begun to discuss how to increase the growth of exogenous growth hormone or promote the secretion of growth hormone...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/42A61K39/395A61P43/00
CPCA61K2039/505A61K2039/552A61P43/00C07K16/4241C07K2317/33C07K2317/35
Inventor 黄威权赵锋吕葆真李丹黄晓文欧阳森
Owner 西安德轩驰生物科技有限公司
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