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A kind of pretreatment liquid of exfoliated cells of rectal mucosa and its preparation method

A technology of pretreatment solution and exfoliated cells, which is applied in botany equipment and methods, preparation of test samples, chemicals for biological control, etc., can solve problems affecting the accuracy of test results, etc., and achieve elimination and inhibition The effect of breeding, ensuring accuracy, and maintaining integrity

Active Publication Date: 2021-09-24
强安医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Mold growth will seriously affect the accuracy of test results

Method used

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  • A kind of pretreatment liquid of exfoliated cells of rectal mucosa and its preparation method
  • A kind of pretreatment liquid of exfoliated cells of rectal mucosa and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 illustrates mold inhibition test

[0025] 1. Configure pretreatment liquids with different disinfectants

[0026] 1. Verification solution 1 (treatment solution before the present invention)

[0027] (1) Dissolve 9g of sodium chloride in 1000ml of distilled water;

[0028] (2) Dissolve 72.8 g of trishydroxymethylaminomethane, 9.3 g of disodium edetate, and 0.2 g of ethyl phenyl polyethylene glycol in the upper liquid;

[0029] (3) Dissolve 65 g of shell iodine and 0.1 ml of methylisothiazolinone in the above liquid and mix well.

[0030] 2. Verification solution 2 (only containing disinfectant)

[0031] (1) Dissolve 9g of sodium chloride in 1000ml of distilled water;

[0032] (2) Dissolve 65g of shell iodine and 0.1ml of methylisothiazolinone in the above liquid and mix well.

[0033] 3. Verification solution 3 (no disinfectant)

[0034] (1) Dissolve 9g of sodium chloride in 1000ml of distilled water;

[0035] (2) Dissolve 72.8 g of trishydroxymethyl...

Embodiment 2

[0062] Example 2 illustrates the observation of cell integrity

[0063] 1. Configure pretreatment fluids with different tensions

[0064] 1. Verification solution 1 (treatment solution before the present invention)

[0065] (1) Dissolve 9g of sodium chloride in 1000ml of distilled water;

[0066] (2) Dissolve 72.8 g of trishydroxymethylaminomethane, 9.3 g of disodium edetate, and 0.2 g of ethyl phenyl polyethylene glycol in the upper liquid;

[0067] (3) Dissolve 65 g of shell iodine and 0.1 ml of methylisothiazolinone in the above liquid and mix well.

[0068] 2. Verification solution 2 (low tension pretreatment solution)

[0069] (1) Take 1000ml of distilled water;

[0070] (2) Dissolve 72.8 g of trishydroxymethylaminomethane, 9.3 g of disodium edetate, and 0.2 g of ethyl phenyl polyethylene glycol in the upper liquid;

[0071] (3) Dissolve 65 g of shell iodine and 0.1 ml of methylisothiazolinone in the above liquid and mix well.

[0072] 3. Verification solution 3 (hi...

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PUM

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Abstract

The invention relates to a pretreatment solution for exfoliated cells of rectal mucosa, which comprises the following components: methylisothiazolinone, shell iodine, trishydroxymethylaminomethane, disodium edetate, ethylphenyl polyethylene glycol Glycols, Sodium Chloride. The present invention also relates to a method for preparing the above pretreatment liquid. The present invention can provide a stable environment for cell samples, and has the effects of preventing coagulation and precipitation of tangible substances in samples, clearing mucus in samples, and reducing viscosity; Microorganisms, especially molds, in the pretreatment process of cell sample detection have the effect of disinfecting and inhibiting growth; The combined application of diol can maintain the integrity and good shape of exfoliated cells of rectal mucosa, thereby ensuring the accuracy of cell sample detection.

Description

technical field [0001] The invention belongs to the field of medical supplies, and in particular relates to a pretreatment solution for rectal mucosal exfoliated cell samples and a preparation method thereof. Background technique [0002] Exfoliative cytology is a branch of cytopathology. It is a discipline that collects epithelial cells from various parts of the human body, observes their morphology with a microscope after staining, and assists in clinical diagnosis of diseases. The advantages of exfoliative cytology examination are: simple and easy to perform, low requirements on equipment, and strong safety; less pain caused to patients, and repeated examination of samples; rapid diagnosis, high detection rate of cancer cells, especially suitable for large-scale Cancer prevention screening and follow-up observation of high-risk groups. Exfoliation cytology of rectal mucosa is one of the important methods for screening rectal cancer. However, the contents of the rectum a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/30A01N25/30A01N43/16A01N43/80A01N59/12A01P3/00A01P1/00
CPCA01N25/30A01N43/16A01N43/80A01N59/12G01N1/28G01N1/30
Inventor 张野杨新财
Owner 强安医疗器械有限公司
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