RPA kit for rapidly detecting mycoplasma synoviae of chicken and its use
A technology of Mycoplasma synovialis and kit, which is applied in the directions of DNA/RNA fragments, recombinant DNA technology, microorganism-based methods, etc., can solve problems such as the application of RPA technology that has not been seen, and achieves reduced possibility, strong specificity, and increased specific effect
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[0033] Embodiment 1 The establishment of a kind of RPA kit and detection method of rapidly diagnosing mycoplasma chicken synovial bursa
[0034] 1. Design and preparation of primers and probe sequences
[0035] The present invention designs primers and probes according to the hypothetical protein VY93_00965 gene of Mycoplasma gallinae bursa; through the comparison and analysis of Blast software in NCBI, it is found that this gene has a high degree of conservation with different strains of Mycoplasma gallisovus uploaded in GenBank The laboratory found that the gene exists in different clinical isolates through proteome sequencing, and the results of amplification and sequencing confirmed that the homology of the gene was as high as 98.5%. Therefore, the present invention selects a section (701bp-920bp) in the full length of the gene as the target fragment to design primers and probes, in order to detect as many Mycoplasma gallisovus as possible. All primers and probes were syn...
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