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Preparation of flagellin vaccine adjuvant-based vaccine to induce production of antibody recognizing conformation of antigens, and application thereof

An antigen recognition and isomer technology, applied in the direction of antigen/adjuvant combination components, antiviral agents, bacterial antigen components, etc., can solve problems such as neuropathic damage

Pending Publication Date: 2019-10-08
李浚行 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is well known, especially in the case of tauopathic Alzheimer's disease induced by changes in the concentration of endogenous proteins, that normally present tau monomers are essential in maintaining the robustness of nerve cell axons, but When hyperphosphorylation is induced by some stimuli, tau polymers in the form of double-stranded helical filaments (PHF, paired helical filament) formed by mutual aggregation induce tau disease and cause pathological damage to other surrounding nerve cells

Method used

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  • Preparation of flagellin vaccine adjuvant-based vaccine to induce production of antibody recognizing conformation of antigens, and application thereof
  • Preparation of flagellin vaccine adjuvant-based vaccine to induce production of antibody recognizing conformation of antigens, and application thereof
  • Preparation of flagellin vaccine adjuvant-based vaccine to induce production of antibody recognizing conformation of antigens, and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Embodiment 1: Preparation of Alzheimer's disease immune vaccine

[0099] Experimental Materials

[0100] Cultivation and storage of each strain

[0101] The Escherichia coli strain used in the present invention was cultured in LB (Luria Bertani) medium (Difco Co.). After culturing, the strain used was added to make the oil content 30%, and then stored in an ultra-low temperature freezer at -80°C.

[0102] The strains and plasmids used in the present invention are listed in Table 1.

[0103] Table 1

[0104]

[0105] Experimental method and experimental results

[0106] 1. Protein expression and purification

[0107] a. Expression and purification of FlaB recombinant protein derived from Vibrio vulnificus

[0108] The flaB recombinant protein was prepared by using the culture gene sequence (SEQ ID NO: 1) of the flagella constituent factor FlaB of Vibrio vulnificus CMCP6. A DNA fragment of 1.1 kbp comprising the FlaB gene for fusion N-terminus or for fusion C-te...

Embodiment 2

[0140] Embodiment 2: the preparation of norovirus immunization vaccine

[0141] a. Antigen sequence and codon optimization of Norovirus P domain

[0142] The DNA used to prepare the antigen for the preparation of the norovirus vaccine used pGEX-4T-1::VAxxx cloned with the norovirus P domain obtained from Professor Cho Kyung-woo of Chonnam University. Insert gene sequence such as SEQ ID NO:12.

[0143] b. Gene Cloning for Preparation of Recombinant Pd Antigen

[0144] In order to obtain the N-terminal of the Pd gene for the antigen or the product DNA fragment for the fusion C-terminal, by using the Pd-N and Pd-C primer pair described in SEQ ID NO:16 sequence 17, to contain The Pd plasmid of SEQ ID NO: 12 was used as a template to amplify a 1.1 kbp DNA fragment. That is, polymerase chain reaction using each primer pair was performed under the conditions of pre-denaturation at 95°C for 5 minutes, followed by 30 cycles of denaturation at 95°C for 30 seconds, annealing at 60°C f...

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Abstract

The present invention provides a vaccine composition for use in neurodegenerative diseases and an infectious virus vaccine composition for inducing an antibody recognizing the conformation of antigens. The vaccine composition of the present invention induces the production of an antibody recognizing the conformation of antigens. The antibody recognizing the conformation of antigens has high specificity for an antigen, and thus can be useful for ameliorating, preventing or treating diseases.

Description

technical field [0001] The invention relates to the preparation and application of a vaccine based on a flagellin vaccine adjuvant that induces the production of conformer antigen recognition antibodies. Background technique [0002] Antibody refers to a substance that specifically binds to an antigen to cause an antigen-antibody reaction immune response. In the immune system, external antigens such as viruses recognize cancer and other internal antigens of their own origin, and the immunoglobulins that specifically bind to simultaneously neutralize or opsonize phagocytosis are called immune antibodies, and the so-called antibodies are usually Refers to the immune antibody that specifically recognizes and binds to a specific antigen. [0003] Antibodies are engulfed and digested by antigen-presenting cells of exogenous or endogenous antigens and delivered to T lymphoma cells to initiate their formation. After selection of a specific fractionated B cell clone for recognitio...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61K39/12C07K14/47A61K39/00
CPCA61K39/00A61K39/02A61K39/12C07K14/47A61P25/28A61P31/18A61K39/107A61K39/0005A61K2039/555A61K2039/55544C07K14/4711A61K2039/55516A61K2039/541A61K35/68A61K9/0019A61K2039/51A61K2039/6075
Inventor 李浚行李施恩郑旷埈朴相哲谭文芝
Owner 李浚行
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