Method for promoting bud differentiation in Qijian white cymbidium sinense tissue culture process

A technology for tissue culture and bud differentiation, applied in the field of plant tissue culture, can solve the problems of low efficiency of factory propagation, difficult rhizome differentiation of intermediate propagules, and inability to meet commercial production, and achieves the technology of promoting bud differentiation and promoting expression. Effect

Pending Publication Date: 2019-10-11
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, Qijian Baimolan has the problem of difficult differentiation of intermediate propagule rhizomes in the tissue c...

Method used

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  • Method for promoting bud differentiation in Qijian white cymbidium sinense tissue culture process
  • Method for promoting bud differentiation in Qijian white cymbidium sinense tissue culture process
  • Method for promoting bud differentiation in Qijian white cymbidium sinense tissue culture process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Yucasin combined with 6-BA promotes the differentiation of rhizomes and shoots of Qijian Baimolan

[0043] (1) Take the rhizome of Qijian Baimolan with a length of 1.0~1.5cm, and inoculate it in 6 treatments of CK, IAA, 6-BA, Yucasin, (Yucasin+IAA) and (Yucasin+6-BA) , each bottle is connected with 10 rhizomes, and each treatment is connected with 21 bottles. After inoculation, place them in an incubator for cultivation, temperature: 26±1°C, light: 2000lux, 12h / d. After photographing at 6 sampling points (5 days, 10 days, 15 days, 20 days, 25 days and 30 days), the differentiation rate of rhizome shoots was counted. The formula for calculating the bud differentiation rate is:

[0044] Bud differentiation rate=(number of rhizomes differentiated from buds / number of connected rhizomes)×100%.

[0045] (2) Culture medium and treatment conditions:

[0046] CK: MS medium + 25.0g / L sucrose + 7.0g / L carrageenan, pH=5.8;

[0047] IAA: MS medium + 25.0g / L sucrose + 7....

Embodiment 2

[0053] Example 2 Yucasin combined with 6-BA promotes the expression of hormone-related genes in the rhizome of Qijian Baimolan

[0054] (1) Take the rhizomes of Qijian Baimolan with a length of 1.0-1.5cm, and inoculate them in the four treatments of CK, 6-BA, Yucasin and (Yucasin+6-BA), and insert 10 roots into each bottle. Each treatment received 7 bottles; after inoculation, they were placed in an incubator (temperature: 26±1°C, light: 2000lux, 12h / d) and cultured at 7 sampling points (0 days, 5 days, 10 days, 15 days, 20 days, 25 days and 30 days) each process randomly gets 1 bottle, totally 10 rhizomes, and is divided into 3 groups at random, every group 3 rhizomes (i.e. a repeat sample) about 0.3 grams, Immediately after sampling, the samples were quickly frozen in liquid nitrogen, and stored in a -80°C refrigerator if no subsequent operations were performed.

[0055] (2) Culture medium and treatment conditions:

[0056] CK: MS medium + 25.0g / L sucrose + 7.0g / L carragee...

Embodiment 3Y

[0070] Example 3 Effects of Yucasin combined with 6-BA on the content of endogenous hormones in the rhizome of Qijian Baimolan

[0071] (1) Take the rhizomes of Qijian Baimolan with a length of 1.0-1.5cm, and inoculate them in the four treatments of CK, 6-BA, Yucasin and (Yucasin+6-BA), and insert 10 roots into each bottle. Shaped stems, each treatment received 3 bottles. After inoculation, place them in an incubator (temperature: 26±1°C, light: 2000lux, 12h / d) for cultivation.

[0072] (2) Media and treatment conditions

[0073] CK: MS medium + 25.0g / L sucrose + 7.0g / L carrageenan, pH=5.8;

[0074] 6-BA: MS medium + 7.0g / L carrageenan + 1.0mg / L 6-BA, pH=5.8;

[0075] Yucasin: MS medium+25.0g / L sucrose+7.0g / L carrageenan+50μM Yucasin, pH=5.8;

[0076] Yucasin+6-BA: MS medium+25.0g / L sucrose+7.0g / L carrageenan+200μM Yucasin+1.0mg / L6-BA, pH=5.8.

[0077] (3) After culturing for 30 days, 3 bottles of materials were taken for each treatment at the sampling point for the deter...

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Abstract

The invention discloses a method for promoting bud differentiation in the Qijian white cymbidium sinense tissue culture process. The rhizome of Qijian white cymbidium sinense is inoculated into a buddifferentiation culture medium to be cultured, the bud differentiation culture medium contains a biosynthetic flaudio-videoonoid monooxygenase specific inhibitor Yucasin containing cytokinin 6-BA andcatalytic auxin, the expression of hormone related genes is promoted, the changes of an endogenous hormone micro-environment are caused, and therefore the bud differentiation of the rhizome of the Qijian white cymbidium sinense is promoted, and the method can be directly used for factory production of Qijian white cymbidium sinense seedlings and promoting the industrial development of cymbidium sinense.

Description

technical field [0001] The invention belongs to plant tissue culture, and specifically relates to a method for promoting bud differentiation in the tissue culture process of Qijian Baimolan. Background technique [0002] Chinese orchid, referred to as Chinese Cymbidium (Chinese Cymbidium), refers to a part of the orchids in the genus Cymbidium (Cymbidium), including Chunlan, Molan, Cymbidium, Jianlan, Hanlan, Chunjian, Lianbanlan, Douban Orchid, sending spring, etc., have extremely high ornamental value, economic value and cultural value. Molan is one of the first domesticated and most widely cultivated species of Chinese orchids. Among them, 'Qijian Baimolan' is one of the four traditional varieties of Molan. Because of its beautiful posture, rich fragrance and distant flower fragrance, it is loved by dignitaries and literati, and is one of the important material carriers of Chinese orchid culture. As China grows stronger, Guolan, as the material carrier of Chinese tradit...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/00
Inventor 曾瑞珍梁钧淞李洁铌郭和蓉张志胜
Owner SOUTH CHINA AGRI UNIV
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