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Seedling culturing method utilizing lateral buds of tissue culture seedlings of hybrid broussonetia papyrifera for propagation expansion

A technology for tissue culture seedlings and lateral buds, which is applied in the field of seedling raising by utilizing hybrid paper mulberry tissue culture seedlings to expand lateral buds, can solve the problems of hindering large-scale popularization, long production cycle and high cultivation cost, and achieves reduction of seedling cost and increase of reproduction coefficient. , the effect of expanding the reproduction coefficient

Inactive Publication Date: 2019-10-18
天水陇新农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, hybrid mulberry trees generally adopt tissue culture and nutrient pot seedling cultivation methods, which have disadvantages such as long production cycle, delicate seedlings, and high cultivation costs, which hinder large-scale promotion

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The present embodiment provides a seedling raising method and a process for expanding lateral buds of hybrid tree seedlings, which specifically include the following steps: (1) Establishment of ear picking nursery for tissue cultured seedlings and cultivation of axillary buds

[0031] 1. Establishment of ear-picking nursery: select fertile farmland with irrigation conditions, apply 1500 kg of high-quality organic fertilizer decomposed livestock and poultry manure per mu, 15 kg of urea (N: more than 46%), ammonium dihydrogen phosphate (P 2 o 5 : 61%, N: 12% or more) 25kg, mixed and spread evenly. After deep plowing, the land is leveled and furrows are made. The furrow width is 4.0m and the furrow length is 20m. Leave a 0.6m working path between the furrows.

[0032] 2. Planting of tissue-cultured seedlings: Optimizing the tissue-cultured seedlings of the hybrid tree 101. The normal and robust tissue culture seedlings with normal growth and development were planted in...

Embodiment 2

[0051] The present embodiment provides a seedling raising method and process for expanding the lateral buds of hybrid tree tissue culture seedlings, which specifically includes the following steps:

[0052] (1) Establishment of ear picking nursery for tissue culture seedlings and cultivation of axillary buds

[0053] 1. Establishment of ear-picking nursery: select a fertile vegetable field with irrigation conditions, apply 1800kg of high-quality organic fertilizer (decomposed livestock and poultry manure), 18kg of urea (N: more than 46%), ammonium dihydrogen phosphate (P 2 o 5 : 61%, N: above 12%) 28kg, mix and spread evenly. After deep plowing, the land is leveled and furrows are made. The furrow width is 4.4m, and the furrow length is adjusted to local conditions. A 0.7m working path is reserved between furrows.

[0054] 2. Colonization of tissue-cultured seedlings: Optimizing the hybrid mulberry 101 seedlings that have undergone explant induction culture, subculture mul...

example 1

[0056] 4. Cultivation of axillary buds and ear picking: in the middle of March in the early spring of the following year, the annual branches grown in the previous year were stubbled at a distance of 15 cm from the ground. In early May, when the new branches grow to 60-100cm, pinch off the top buds in time to force the axillary buds to germinate one after another. From early June to early July, when the germinated axillary buds grow to 15-20 cm or 6-8 leaves, and the base of the axillary buds is semi-lignified, the axillary buds can be harvested and cultured in vitro.

[0057] (2) Seedbed (nutrient bowl) substrate and rooting agent configuration

[0058] 1. Seedbed matrix and nutrient bowl matrix formula: The nutrient bowl matrix is ​​composed of peat soil, perlite, vermiculite, and loess in a ratio of 5:2:2:1. Use 12g / m 3 20% Dibendazim WP mixed for disinfection. The substrate is made into a nutrient bowl or a seedbed with a width of 90 cm, a height of 9 cm and a length of...

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Abstract

The invention relates to the technical field of propagation of seedlings of hybrid broussonetia papyrifera, in particular to a seedling culturing method utilizing lateral buds of tissue culture seedlings of the hybrid broussonetia papyrifera for propagation expansion. The method comprises the following steps of (1) stumping of the tissue culture seedlings for promoting tillering; (2) disbudding for promoting growth of axillary buds; (3) breaking-off of the axillary buds; (4) a formula of a rooting agent and axillary bud treatment; (5) axillary bud cuttage; construction of a tissue culture seedling cutting garden; seedbed substrate protection after breaking-off of the axillary buds in the step (3); seedbed management after axillary bud cuttage in the step (5), seedling hardening; transplanting to a large seedling garden and seedling garden management. According to nursey stocks cultured by adopting the culturing method, based on the height of the nursey stocks, the advantages of the seed nature characteristics of the tissue culture seedlings, growth and development of newly-born bodies and stress resistance and disease and pest resistance are kept, and due to culturing in the seedling garden, plants of the type are robust and tall, have significantly improved drought resistance, cold resistance and overgrown weed resistance, and are especially suitable for barren mountain afforestation, ecological construction and forage grass planting and capable of quickly achieving the yield purpose when taken as forage grass for planting.

Description

technical field [0001] The invention relates to the technical field of breeding of hybrid tree seedlings, in particular to a method for growing seedlings by utilizing the tissue culture of hybrid tree seedlings to propagate side buds. Background technique [0002] Hybrid mulberry tree is a high-quality tree species that can be planted in most parts of China after ten years of painstaking research and demonstration verification by the Institute of Botany, Chinese Academy of Sciences. This variety has the characteristics of fast growth, high yield, rich nutrition, smooth leaves and good palatability. The crude protein content in the leaves is as high as 26%, which is 1.4 times that of alfalfa. , The harvest process does not dig pits, does not disturb the soil layer, and the water and soil conservation effect is good. In view of the important role of hybrid mulberry in feed, papermaking, barren hills greening and ecological restoration. At present, a large number of planting ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G17/00A01G2/10A01G24/10A01G24/15A01G24/28
CPCA01G17/005A01G2/10A01G24/10A01G24/15A01G24/28
Inventor 袁米丽金茜陈文文王煜周碧霞
Owner 天水陇新农业科技发展有限公司