Application of hydrogen-rich water composition in inhibition of hexavalent chromium-induced endoplasmic reticulum stress and autophagy in DF-1 cells

Abstract

The invention discloses application of a hydrogen-rich water composition in inhibition of hexavalent chromium-induced endoplasmic reticulum stress and autophagy in DF-1 cells. The hydrogen-rich watercomposition comprises 0.6-1.1 mM of H2, 1.8-2.4 g / L of sodium alginate and 1.2-1.6 g / L of mannan. The hydrogen-rich water composition can maintain the stability of hydrogen content in hydrogen-rich water; moreover, the hydrogen-rich water composition can achieve an inhibition effect on the increase of the expression amount of COX-2 induced by Cr(VI) in the DF-1 cells, thereby inhibiting endoplasmic reticulum stress and excessive autophagy caused by the increase of COX-2 in the DF-1 cells.

Description

Technical field [0001] The present invention relates to the technical field of medical uses of hydrogen-rich water compositions, in particular to the application of hydrogen-rich water compositions in inhibiting hexavalent chromium-induced endoplasmic reticulum stress and autophagy in DF-1 cells. Background technique [0002] The endoplasmic reticulum (ER) is a precise membrane system in living cells, which is widely present in eukaryotic cells and participates in protein folding and maintains calcium homeostasis. Efficient ER function is essential for normal cell activity. When ER function is disturbed by some internal or external stimuli such as ischemia, hypoxia, nutritional deficiency, homocysteine, etc., the endoplasmic reticulum cannot perform its normal physiological functions. When endoplasmic reticulum stress occurs, unfolded proteins or misfolded proteins will accumulate, and when these proteins accumulate to a certain extent, they will give a feedback signal to the en...

AI Technical Summary

Problems solved by technology

However, there is still a lack of relevant research on how to inhibit the increase of COX-2 in DF-1 cells caused by hexavalent chromium, and the endoplasmic reticulum stress and excessive autophagy in DF-1 cells caused by the increase of COX-2

However, there are no reports about hydrogen-rich water inhibiting the increase of COX-2 in DF-1 cells caused by hexavalent chromium, and the endoplasmic reticulum stress and excessive autophagy of DF-1 cells caused by the increase of COX-2

[0006] Moreover, due to the small molecular weight of hydrogen and other reasons, hydrogen permeability is extremely strong. Therefore, hydrogen is very easy to diffuse through packaging materials and tiny gaps, so that the hydrogen content in hydrogen-rich products is reduced, or even does not contain hydrogen, giving hydrogen-rich products The storage and transportation bring great difficulties, which limits the popularization and application of hydrogen-rich products

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