DNA detection PCR fluorescent analysis system

Abstract

The invention discloses a DNA detection PCR fluorescent analysis system. A fluorescent group is added into a PCR reaction system, PCR amplification and detection processes are combined and performed synchronously in the same closed container, an amplification product is detected after each PCR cycle, a reactant amplification curve is obtained, finally, the relative quantitative method is used forperforming quantitative analysis on an unknown template, the cycle index Ct value that the unknown template reaches the designated fluorescent strength or the fluorescent strength percentage is determined, and the concentration of the unknown template is determined according to the Ct value, wherein the measured cycle index Ct values of amplification reagents in different batches are corrected, and the DNA detection results of amplification reagents in different batches have the consistency.

Description

technical field [0001] The invention relates to the technical field of DNA detection, in particular to a polymerase chain reaction (PCR) fluorescence analysis system for DNA detection. Background technique [0002] As the basic genetic material, DNA nucleic acid carries a variety of information, and the understanding of its structure and function is conducive to the study of heredity, evolution and disease diagnosis of species. Polymerase chain reaction (PCR) technology is a nucleic acid amplification technology that simulates the natural DNA replication process in vitro, also known as cell-free amplification technology. Using this technology, trace amounts of DNA genetic material can be rapidly and simply multiplied by one hundred More than ten thousand times. Due to the high sensitivity and high accuracy of this technology, it has been widely used in biomedical fields, such as medical diagnosis, food safety, animal quarantine and genetics research. [0003] Fluorescent q...

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Problems solved by technology

However, in this invention, the Ct detection value is corrected by changing the intermediate parameters, rather than the direct correction of the Ct detection value; because the reference point of the response curve is usually located at the larger part of the response curve slope, the smaller curve difference will also If the test reagent has a large error at the expected value of the standard reagent, the error of the corrected benchmark point will increase, thus affecting the test result.

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