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Castanea henryi regeneration plant and ectomycorrhizal fungi symbiosis method

A technology for ectomycorrhizal and regenerated plants, applied in the field of cultivation, can solve the problems of small size, plant root adversity stress, and the research of mycorrhizal symbiotic process, and achieve the effect of improving accuracy and repeatability, and facilitating research

Inactive Publication Date: 2019-10-25
CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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Problems solved by technology

However, this method also has serious defects, because the root system is only partly in contact with the surface of the medium, the root system cannot grow normally and freely and the growth space is quite narrow, which will cause adversity stress to the plant root system and affect the mycorrhizal growth to a large extent. The study of symbiotic processes

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  • Castanea henryi regeneration plant and ectomycorrhizal fungi symbiosis method

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[0036] In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described here, and those skilled in the art can make similar improvements without departing from the connotation of the present invention, so the present invention is not limited by the specific implementations disclosed below.

[0037] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the technical field of the invention. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention.

[0038] As used herein, the term "MS" medium is a relatively stable ion-balanced solution with a high concentration of inorganic salts and ions. It ha...

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Abstract

The invention relates to a castanea henryi regeneration plant and ectomycorrhizal fungi symbiosis method, including the steps of S1, cultivation of aseptic seedlings of castanea henryi; S2, building of a regeneration system of castanea henryi; S3, cultivation of ectomycorrhizal fungi; and S4, symbiosis cultivation. The building of the regeneration system of castanea henryi can provide a guaranteeto uniform genetic background and improve experimental result accuracy and repeatability. The symbiosis system brings convenience to statistic and observation on the castanea henryi mycorrhizal infection process and change of the root structure caused by ectomycorrhizal fungi mycorrhizal infection and to research on mycorrhiza growth promotion effect.

Description

technical field [0001] The invention relates to the field of cultivation technology, and more specifically relates to a method for the symbiosis of the regenerated plant of the chestnut chestnut and the ectomycorrhizal fungus. Background technique [0002] The infection rate of ectomycorrhizal (ECM) fungi in Fagaceae can be as high as over 90%, and there is extremely rich diversity of ECM fungi in the southern and northern hemispheres. Castanea henryi is a plant of the genus Castanea in the family Fagaceae of the order Fagales. It originated in China and is one of the important woody food tree species in China. It is mainly distributed in the south of the Yangtze River in my country. Chestnut plants mainly grow in nutrient-poor mountains, hills or alluvial sandy land of river beaches. Therefore, at present, the research on the mycorrhizal effect of Chestnut is mainly concentrated on the absorption of mineral nutrients (N, P), photosynthesis of leaves, resistance to drought ...

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Application Information

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IPC IPC(8): A01G31/00A01G18/10A01G24/28A01G24/12A01H4/00
CPCA01G31/00A01H4/008A01G18/10A01G24/12A01G24/28
Inventor 袁德义熊欢邹锋孙贺范晓明毛小雪王荣洁
Owner CENTRAL SOUTH UNIVERSITY OF FORESTRY AND TECHNOLOGY
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