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Method for creating tomatoes parasitizing on orobanche cumana wallr. by utilizing indoor culture dish system

A sunflower and petri dish technology, applied in the biological field, can solve the problems of short time, limitation and high efficiency of sunflower, and achieve the effect of reducing workload, low cost and reducing cost

Inactive Publication Date: 2019-11-01
INNER MONGOLIA AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, because the sunflower used to identify the level of anti-retides is a sunflower seedling with 8 to 10 true leaves, the time for cultivating sunflowers required for the identification is short and the efficiency is high, and the rapid detection of the anti-retides level of sunflowers is realized. However, the patent also uses sunflower and radang as the research objects. Due to the immaturity of the genetic transformation system of sunflower and radang, it is limited to analyze the resistance mechanism of sunflower and the pathogenic mechanism of radang at the molecular level.

Method used

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  • Method for creating tomatoes parasitizing on orobanche cumana wallr. by utilizing indoor culture dish system
  • Method for creating tomatoes parasitizing on orobanche cumana wallr. by utilizing indoor culture dish system
  • Method for creating tomatoes parasitizing on orobanche cumana wallr. by utilizing indoor culture dish system

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Experimental program
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Effect test

Embodiment 1

[0060] In order to achieve the above object, the technical scheme adopted in the present invention is:

[0061] A kind of method utilizing indoor petri dish system to set up sunflower rowdang parasitic tomato, specifically comprises the following steps:

[0062] 1. Selection of tomato varieties

[0063] The tomato lines suitable for the present invention are 54♀, 966♀ and 1104.

[0064] 2. Tomato planting

[0065] Mix the seedling-raising matrix with water, and put it into a 98-hole seedling-raising tray. Sow tomato seeds into seedling holes, sowing 3 seeds per hole. Cultivate at 25°C under 16h light / 8h dark conditions, water as needed, and use for inoculation after 3 weeks of cultivation.

[0066] 3. Obtaining Filter Paper Containing Sunflower Root Exudates in a Petri Dish

[0067] After two weeks of tomato cultivation, the Petri dishes were pretreated to obtain Petri dishes containing sunflower root exudates - filter paper. The seeds of sunflower LD5009 were rinsed und...

Embodiment 2

[0073] The invention discloses a method for establishing the sunflower raddang parasitic tomato using an indoor petri dish system. In the process of establishing the system, it is particularly important to obtain filter paper containing sunflower root exudates. This embodiment is the same as Example 1, the difference is that a screening stage is also included before obtaining the filter paper containing sunflower root exudates in the petri dish in step (3): watering with clear water, sunflower root exudates watering and pre-treatment in the petri dish Germination of sunflower seeds Obtaining filter paper containing sunflower root exudates Three methods were used to explore optimal parasitic conditions.

[0074] Specifically, with the sunflower host as a control, by Figure 7 It can be found that, compared with the control, the germination rate of sunflower rowdang was significantly lower by water irrigation and sunflower root exudates irrigation than that of the control group,...

Embodiment 3

[0076] A method for establishing a sunflower raddang parasitic tomato using an indoor petri dish system is similar to that of Example 1, except that in step (2), it is cultivated at 22° C. under the conditions of 12 hours of light / 6 hours of darkness.

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Abstract

The invention discloses a method for creating tomatoes parasitizing on orobanche cumana wallr. by utilizing an indoor culture dish system. The method comprises the following steps of (1) selecting a tomato variety; (2) planting tomato seeds; (3) obtaining secreta of a sunflower root system; (4) inoculating tomatoes to orobanche cumana wallr.; (5) observing and counting the germination rate of seeds of orobanche cumana wallr. and the number of parasitic nodules. The system established by the method for efficient parasitism of the tomatoes on orobanche cumana wallr. can be used for researching the functions of disease-resistant candidate genes in sunflowers by means of the tomatoes, and the functions of pathogenic candidate genes in orobanche cumana wallr. can be researched by establishing a'parasitic bridge' of the tomatoes and orobanche cumana wallr. to interfere with the pathogenic candidate genes in orobanche cumana wallr.. Therefore, the establishment of the system provides a new way for the subsequent research on the molecular mechanism of interaction between sunflowers and orobanchaceae.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for establishing sunflower rowan parasitic tomato using an indoor culture dish system. Background technique [0002] Sunflower cumana (Orobanche cumana Wallr.) is an annual parasitic angiosperm of the genus Orobanche in the family Orobanchaceae. It has caused great damage to the production of sunflower for a century. Liedang parasitizes on the root of the host plant, the stem is fleshy, covered with fine hairs, erect, cylindrical, 20-40cm high, solitary, rarely branched. The leaves degenerate into light yellow scales, arranged in a spiral shape on the stem, do not contain chlorophyll, and cannot perform photosynthesis to produce organic matter. When grown well, it is bulbous at the base and bears inflorescences in the upper third of the stem. Since it has no true roots, it cannot obtain nutrients from the soil, so it can only rely on its host to provide nutrients. [0003...

Claims

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Application Information

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IPC IPC(8): A01G22/00A01G22/05A01G7/06A01G9/029A01C1/02
CPCA01C1/02A01G7/06A01G9/029A01G22/00A01G22/05
Inventor 张键赵君柳慧卿石胜华王娜刘志达
Owner INNER MONGOLIA AGRICULTURAL UNIVERSITY
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