Coconut extract and use thereof
A technology of extract and coconut, applied in the field of coconut extract, can solve the problems of polluted environment, high cost, impact on food safety, etc., and achieve the effects of simple disinfection method, high inhibition rate and short bacteriostatic time.
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[0025] Concrete embodiment steps are as follows:
[0026] 1. Materials and methods
[0027] 1.1 Test strains
[0028] The diseased mulberries were collected from the mulberry trees of the Sericulture Research Institute of the Chinese Academy of Agricultural Sciences, and the pathogenic bacteria were isolated and purified. Primers were designed according to the ribosomal ITS sequence of the main pathogen of mulberry sclerotinia. After PCR amplification, sequencing, and sequence comparison, the isolation and purification were identified. The pathogen is Sclerotinia sclerotiorum (see figure 1 ). The identified pathogenic bacteria were propagated on PDA medium and stored in a refrigerator at 4°C.
[0029] 1.2 The fungicide to be tested The fungicide to be tested is coconut extract, provided by Hierzi (Shanghai) Biotechnology Co., Ltd., and the trade name is "Edible Disinfectant".
[0030] 1.3 Sterilization of equipment and reagents
[0031] Sterilize the required equipment, t...
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