LncRNA marker and application of LncRNA marker to diabetes

A technology for markers and diabetes, applied in the direction of DNA/RNA fragments, recombinant DNA technology, microbial determination/inspection, etc.

Pending Publication Date: 2019-11-01
徐州市疾病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are no reports on the differential expression, diagnostic value, and mechanism of action of lncRNA in diabet

Method used

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  • LncRNA marker and application of LncRNA marker to diabetes
  • LncRNA marker and application of LncRNA marker to diabetes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1. Sampling: Collect 5 mL of anticoagulated blood from 107 diabetic patients (experimental group) with blood sugar ≧7.1mmol / L, and 5 mL of anticoagulant blood from 107 normal people without diabetes or other diseases (control group), and send them within 1 hour To the laboratory, all samples were obtained before the patient's informed consent, and all approved by the organization ethics committee. Add 3 times the red blood cell lysate of whole blood to the samples obtained above, mix upside down and let stand at room temperature for 10 min, centrifuge at 3000 rpm for 5 min, discard the supernatant, add 2 mL of red blood cell lysate to the pellet again, mix well, and stand at room temperature Leave for 10 min, centrifuge at 3000 rpm for 5 min, and discard the supernatant. Add 2mL Trizol to the precipitate to dissolve it, use a pipette tip to blow until the solution is not sticky, and quickly store it in a -80℃ refrigerator.

[0028] 2. Extraction of white blood cell total ...

Embodiment 2

[0041] Example 2: Expression of marker LncRNA in diabetic tissue

[0042] According to the PCR amplification results of Example 1, using SYBR Green as the fluorescent marker, the PCR reaction was performed on the Light Cycler fluorescent quantitative PCR instrument, the target band was determined by melting curve analysis and electrophoresis, and the ΔΔCT method was used for relative quantification.

[0043] The experiment was repeated 3 times, and the result data was expressed in the form of mean±standard deviation. The SPSS18.0 statistical software was used for statistical analysis. The paired comparison between the normal population control group and the diabetic population experimental group used t-test, which was considered as P It is statistically significant when figure 2 As shown, the expression level of NONHSAT024449 gene in the blood of the experimental group of diabetic patients was significantly lower than that of the control group of normal people, showing a low expres...

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Abstract

The invention belongs to the field of biological detection and particularly relates to an LncRNA marker and application of the LncRNA marker to diabetes. It is successfully detected that the LncRNA marker NONHSAT024449 has the obvious expression difference in blood of diabetic patients, compared with a normal crowd contrast group, the patients of a diabetic crowd experiment group have the low-expression LncRNA marker NONHSAT024449, and based on the discovery, the LncRNA marker NONHSAT024449 can serve as a diabetes molecular marker or target to be applied to diabetes clinical diagnosis or targeted therapy; by detecting a specific primer pair of the LncRNA marker NONHSAT024449, early screening and prevention of diabetes/evaluation of the occurrence risk of diabetes can be realized; and by applying the LncRNA marker NONHSAT024449 to preparation of a diabetes diagnosis product or a diabetes prevention/ evaluation product, diabetes occurrence and development mechanisms, signal channels andthe like are further illuminated advantageously, and the LncRNA marker extremely has application prospects and theoretical value.

Description

Technical field [0001] The invention belongs to the field of biological detection, and specifically relates to a LncRNA marker and its application in diabetes. Background technique [0002] Diabetes is a multi-cause metabolic disease characterized by high blood sugar, which can cause chronic damage and dysfunction of various tissues, especially eyes, kidneys, heart, blood vessels, and nerves. The occurrence and development of diabetes is a chronic process, and the early symptoms are not obvious. Existing studies have shown that long-chain non-coding RNA (LncRNA) can affect the occurrence and development of diseases by regulating mRNA transcription, translation or modification. PCR has a high degree of specificity and sensitivity, and can reflect small changes through intuitive numerical values. There have been researches on the detection of related genes or LncRNA through PCR and other means, but they are mainly concentrated in diseases such as nervous system diseases, cardiova...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883C12N15/113
CPCC12Q1/6883C12Q2600/158C12Q2600/178
Inventor 娄培安张盼杜阳光
Owner 徐州市疾病预防控制中心
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