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Extraction and detection method for Angelica amurensis volatile oil and application

The technology of an extraction method and a detection method, which is applied in the field of extraction, analysis and detection of traditional Chinese medicine, can solve the problems of human health and environmental hazards, and achieve the effect of improving yield and simple process

Pending Publication Date: 2019-11-01
TONGHUA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some synthetic active ingredients or preservatives have been reported to be harmful to human health and the environment

Method used

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  • Extraction and detection method for Angelica amurensis volatile oil and application
  • Extraction and detection method for Angelica amurensis volatile oil and application
  • Extraction and detection method for Angelica amurensis volatile oil and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Cut 630g of fresh root of Angelica sinensis, add 3000mL of distilled water to a 5000mL distillation bottle, soak, connect to a steam distillation device, heat and extract, and collect 4mL of volatile oil of Angelica sinensis, with a yield of 0.63%. The soaking temperature is 20°C, and the soaking time is 3 hours; the extraction temperature in step (1) is 100°C, and the extraction time is 6 hours; the extraction liquid-solid ratio in step (1) is 10:1.

Embodiment 2

[0018] Gas chromatography-mass spectrometry was used to detect and analyze the extracted volatile oil; the chromatographic conditions: the carrier gas was helium, and the injection volume was 1 , the inlet temperature is 220°C, the split ratio is 1:10; the temperature rise program is set as follows: the initial temperature is 40, hold for 2 minutes, then rise to 250 at 5 / min, hold for 5 minutes, and the flow rate is set to 1.0mL / min; the mass spectrometry conditions are EI ionization source, electron bombardment ionization voltage is 70eV, m / z 50-500, ion source temperature is 230; figure 1 , Table 1 shows. It can be seen from Table 1 that a total of 38 compounds were isolated and identified, among which α-pinene, -pinene, (-)- -pinene, (+)-sylvestrene, L-bornyl acetate and hedycaryol are its main components.

Embodiment 3

[0020] Determination of hypoglycemic experiment:

[0021] take 20 5-(1,2-dithiolan-3-yl)-N-(4-hydroxyphenyl)pentanamide solution was added to a 96-well plate, and 100 µL α-glucosidase solution was added, 80 0.1M PBS, incubate at 25°C for 10 minutes. then add 50 p-nitrophenyl-α-D-glucopyranoside (pNPG) solution, incubated at 25°C for 5 minutes. The absorbance before and after incubation was measured at 405 nm using a microplate reader. Acarbose was used as a positive control. The α-glucosidase inhibitory activity is expressed as % inhibition, and the calculation formula is as follows:

[0022]

[0023] All experiments were performed three times, and the data were analyzed using SPSS software and Origin software to calculate the IC 50 72.3±0.7 (The same concentration of acarbose IC 50 is 39.3±0.2). The conclusion is that the volatile oil of Heishui Angelica sinensis has a hypoglycemic activity close to that of the positive control drug acarbose.

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Abstract

The invention belongs to the technical field of traditional Chinese medicine extraction and analysis detection, relates to an extraction and detection method for Angelica amurensis volatile oil and application, and also relates to application of Angelica amurensis volatile oil in the aspect of reducing blood glucose. The extraction and detection method comprises the following steps: extracting volatile oil by using a steam distillation method, cutting the roots of fresh Angelica amurensis, putting the roots in a reactor, adding distilled water, and soaking, wherein the soaking temperature is 20 to 40 DEG C, and the soaking time is 1 to 5 h; connecting a steam distillation device, and carrying out heating digestion, wherein the digestion extraction temperature is 80 to 180 DEG C, the extraction time is 1 to 8 h, and the extraction liquid-solid ratio is (4 to 20): 1; and collecting the volatile oil. The invention also discloses application of Angelica amurensis volatile oil in preparation of medicine and food for reducing blood glucose. The technology of using the steam distillation method is simple, environment and pollution-free, the yield of the volatile oil is improved through optimization and improvement, and the prepared volatile oil has the activity of reducing blood glucose.

Description

technical field [0001] The invention belongs to the technical field of extraction, analysis and detection of traditional Chinese medicines, relates to a method for extracting and detecting volatile oil of angelica sinensis, and also relates to the application of the essential oil of angelica sinensis in reducing blood sugar. Background technique [0002] In the prior art, black water angelica ( Angelica amurensis Schischk) is angelica, which is traditionally used to treat wind-chill headaches, sinusitis, swollen and painful gums, and skin infections. The root is used for medicinal purposes, and current research focuses on the isolation of constituents and the study of antipyretic, analgesic, and anti-inflammatory activities. From A. amurensis Three compounds, sit-sitosterol, xanthine and umbelliferone, were isolated from respectively from A. amurensis Five coumarin compounds were obtained from the study, named umbelliferone, bergapten, imperatorin, isoimperatorin and x...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/72G01N30/06G01N30/12A61K36/232A61P3/10
CPCG01N30/02G01N30/72G01N30/06G01N30/12A61K36/232A61P3/10G01N2030/067G01N2030/122G01N2030/126
Inventor 臧皓张露云徐倩李丽耿晓宇夏广清朱俊义
Owner TONGHUA NORMAL UNIV
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