41results about How to "Hypoglycemic activity" patented technology

The invention discloses a rosa roxburghii polysaccharide, as well as a preparation method and application thereof. The preparation method mainly comprises raw material pretreatment, extraction, deproteinization, de-coloration, alcohol precipitation, as well as separation and purification, wherein the extraction is to mix pretreated rosa roxburghii dry powder and water in a mass volume ratio of (1to 15) to (1 to 35), and perform extraction at a temperature of 55 to 95 DEG C; perform centrifugal separation to obtain a rosa roxburghii polysaccharide extracting solution, and concentrate the rosaroxburghii polysaccharide extracting solution under reduced pressure to 1 / 3 to 1 / 6 of the original volume to obtain a concentrated solution of the rosa roxburghii polysaccharide. The rosa roxburghii polysaccharide prepared by the invention has the advantages of high purity and uniform molecular weight. Compared with the existing rosa roxburghii polysaccharide, as proved by in-vitro fermentation experiments, the rosa roxburghii polysaccharide disclosed by the invention can promote the generation of short-chain fatty acids such as acetic acid, propionic acid and butyric acid, promote the growthof beneficial bacteria such as lactic acid bacteria and bifidobacterium, and can be taken as prebiotics. Meanwhile, the rosa roxburghii polysaccharide can inhibit the activity of alpha-glucosidase, and can be applied to healthy foods or medicines having auxiliary therapy effects on intestinal diseases and diabetes mellitus.

The invention discloses a method for preparing a polygalacturonic acid-based nano oral protein drug delivery system and application thereof. The system is prepared by adopting a W / O / W double emulsion method. The method comprises the following steps: dissolving polygalacturonic acid in a 4-6w / v% poloxamer 188 solution, and adding bovine insulin for dissolving, thereby obtaining an inner water phase; taking ethyl acetate as an oil phase, respectively taking 18-22w / v% poloxamer 188 solution, 18-22w / v% poloxamer 188 solution dissolved with chitosan and 18-22w / v% poloxamer 188 solution dissolved with chitosan and genipin as outer water phase solutions, and preparing to obtain different nano oral protein drug delivery systems. The nano oral protein drug delivery system disclosed by the invention is non-toxic, easy to degrade, high in delivery rate and high in absorption rate, and has high application prospects in the fields such as delivery research and application of oral protein drugs such as insulin, diabetes treatment, and vaccine administration.

The invention discloses Undaria pinnatifida polysaccharides and applications of the Undaria pinnatifida polysaccharides in the preparation of alpha-glucosidase activity inhibition drugs. The characteristic molecular weights of the two polysaccharides Up-3 and Up-4 are 78-282 kDa and 41-314 kDa respectively; the Up-3 is mainly composed of glucuronic acid, mannose and fucose, and the Up-4 is mainlycomposed of rhamnose, glucuronic acid and fucose; and the IC50 values of the alpha-glucosidase inhibitory activity of the Up-3 and Up-4 are 113.40 + / -3.02 [mu]g / mL and 50.53 + / -3.47 [mu]g / mL respectively, and the Up-3 and Up-4 have the effect of remarkably inhibiting blood glucose rise caused by oral maltose. Therefore, the sulfated polysaccharides Up-3 and Up-4 can reduce the postprandial blood sugar level by inhibiting the activity of alpha-glucosidase in intestinal tracts so as to achieve the effect of reducing blood sugar.

The invention discloses a rosa roxburghii polysaccharide, as well as a preparation method and application thereof. The preparation method mainly comprises raw material pretreatment, extraction, deproteinization, de-coloration, alcohol precipitation, as well as separation and purification, wherein the extraction is to mix pretreated rosa roxburghii dry powder and water in a mass volume ratio of (1to 15) to (1 to 35), and perform extraction at a temperature of 55 to 95 DEG C; perform centrifugal separation to obtain a rosa roxburghii polysaccharide extracting solution, and concentrate the rosaroxburghii polysaccharide extracting solution under reduced pressure to 1 / 3 to 1 / 6 of the original volume to obtain a concentrated solution of the rosa roxburghii polysaccharide. The rosa roxburghii polysaccharide prepared by the invention has the advantages of high purity and uniform molecular weight. Compared with the existing rosa roxburghii polysaccharide, as proved by in-vitro fermentation experiments, the rosa roxburghii polysaccharide disclosed by the invention can promote the generation of short-chain fatty acids such as acetic acid, propionic acid and butyric acid, promote the growthof beneficial bacteria such as lactic acid bacteria and bifidobacterium, and can be taken as prebiotics. Meanwhile, the rosa roxburghii polysaccharide can inhibit the activity of alpha-glucosidase, and can be applied to healthy foods or medicines having auxiliary therapy effects on intestinal diseases and diabetes mellitus.

A preparation method of a pumpkin polysaccharide hydrogel composite with a hypoglycemic effect includes the steps of adding pumpkin polysaccharide powder into a dry reaction still, adding ethyl alcohol to be stirred at 25 DEG C, dissolving NaIO4 with weight accounting for 0.5-0.50 time of the weight of pumpkin polysaccharide in water to prepare a saturated solution, slowly adding the saturated solution into a reaction system for reaction in a light shielded state, adding ethylene glycol into the reaction system to be stirred, adding absolute ethyl alcohol into the reaction system to be stirred and then subjected to suction filtration, conducting dialysis for 72 hours through a dialysis bag with molecular weight cut-off of 3500, conducting drying for 12 hours at 50 DEG C to obtain oxidized pumpkin polysaccharide, weighing the oxidized pumpkin polysaccharide, dissolving the oxidized pumpkin polysaccharide in distilled water, weighing N,O-carboxymethyl chitosan with weight accounting for 2-4 times of the weight of the oxidized pumpkin polysaccharide, dissolving N,O-carboxymethyl chitosan in distilled water according to the weight volume ratio of 1:10, mixing the two solutions in equivalent volume, and standing for 10 minutes at room temperature to obtain pumpkin polysaccharide hydrogel with the hypoglycemic effect.

The invention discloses a process method for extracting stevia rebaudiana polyphenol from stevia rebaudiana. The process method comprises the following steps: washing fresh stevia rebaudiana, weighing, and pulping with a beater to obtain slurry; standing the slurry in an acid solution having a certain pH value for a period, filtering with gauze to obtain slag; performing ultrasonic extraction on the slag by using alcohol as an extracting liquid to obtain crude extract; extracting the crude extract by using supercritical carbon dioxide extraction technology to obtain a stevia rebaudiana polyphenol extract; and purifying the stevia rebaudiana polyphenol extract to obtain stevia rebaudiana polyphenol powder. The process method can be used for singly and specifically extracting stevia rebaudiana polyphenol from stevia rebaudiana, has relatively high extracting purity and extraction rate, is simple in operation, and is suitable for industrial production for stevia rebaudiana polyphenol.

The invention belongs to the technical field of traditional Chinese medicine extraction and analysis detection, relates to an extraction and detection method for Angelica amurensis volatile oil and application, and also relates to application of Angelica amurensis volatile oil in the aspect of reducing blood glucose. The extraction and detection method comprises the following steps: extracting volatile oil by using a steam distillation method, cutting the roots of fresh Angelica amurensis, putting the roots in a reactor, adding distilled water, and soaking, wherein the soaking temperature is 20 to 40 DEG C, and the soaking time is 1 to 5 h; connecting a steam distillation device, and carrying out heating digestion, wherein the digestion extraction temperature is 80 to 180 DEG C, the extraction time is 1 to 8 h, and the extraction liquid-solid ratio is (4 to 20): 1; and collecting the volatile oil. The invention also discloses application of Angelica amurensis volatile oil in preparation of medicine and food for reducing blood glucose. The technology of using the steam distillation method is simple, environment and pollution-free, the yield of the volatile oil is improved through optimization and improvement, and the prepared volatile oil has the activity of reducing blood glucose.

The invention provides a new high B-ring berberine and palmatine derivative. The structural formula is shown in the description. The invention also provides a preparation technology and application ofthe high B-ring berberine and palmatine derivative. Pharmacodynamic tests prove that the compound provided by the invention has a relatively good effect of reducing blood glucose in vitro and is partially superior to a positive control of berberine hydrochloride. The high B-ring berberine and palmatine derivative provided by the invention has a remarkable drug effect and possibly provides a new clinical medication option.

The invention belongs to the field of medicine, and particularly relates to an oral pharmaceutical composition for treating diabetic. The oral pharmaceutical composition is prepared from, by weight, pharmaceutically acceptable excipients, 11-23 parts of kava piperine B and 5-10 parts of tartaric acid pimavanserin. The oral pharmaceutical composition is granules preferably. Animal experiments showthat a compound of kava piperine B and tartaric acid pimavanserin has good antidiabetic effect and safety.

The invention provides nanoparticles capable of assisting in lowering blood sugar and a preparation method thereof, belonging to the technical field of biological pharmacy. A gulfweed oligosaccharide-stevia rebaudian leaf tea preparation solution used as a water phase and soybean lecithin used as a surfactant are compounded with a red yeast citraurin ethanol solution to obtain gulfweed functional oligosaccharide-citraurin nanoparticles capable of assisting in lowering blood sugar. The nanoparticles have the effect of assisting in lowering blood sugar. By using the nano carrier for embedment, the nanoparticles have the effects of enhancing the stability of blood-sugar-lowering active components, prolonging the acting time and enhancing the blood-sugar-lowering action, and obviously enhance the bioavailability of the functional oligosaccharides and pigments.

The invention discloses a wakame polysaccharide and its application in the preparation of α-glucosidase activity inhibiting drugs. The two polysaccharides Up-3 and Up-4 prepared by the present invention have characteristic molecular weights of 78-282 kDa and 41-314 kDa respectively; Up-3 is mainly composed of glucuronic acid, mannose and fucose, and Up-4 is mainly composed of Composition of rhamnose, glucuronic acid and fucose; IC for α-glucosidase inhibitory activity 50 The values ​​were 113.40±3.02μg / mL and 50.53±3.47μg / mL, respectively, and significantly inhibited the increase in blood sugar caused by oral administration of maltose. This shows that the sulfated polysaccharides Up-3 and Up-4 can reduce the level of postprandial blood sugar by inhibiting the activity of α-glucosidase in the intestine, thereby achieving the hypoglycemic effect.

The present invention relates to a process for isolating plant Argyrobium roseum extract that contains flavonoid glycoside, wherein the extract possesses hypoglycaemic activity. The present invention also contemplates a composition containing the extract and a method of treating various hyperglycaemic conditions including non-insulin dependent diabetes mellitus disease condition by administering the extract.

The invention discloses a traditional Chinese medicine formula based mild medicinal liquor for treating ozostomia and a preparation method thereof. The medicinal liquor is prepared from the following components in parts by weight: 20-23 parts of rhizoma polygonati, 50-60 parts of honey, 20-23 parts of herba epimedii, 15-17 parts of pericarpium citri reticulatae viride, 15-17 parts of coptis chinensis, 15-17 parts of astragalus membranaceus, 15-17 parts of licorice roots, 20-23 parts of gypsum, 15-17 parts of apricot kernels, 13-15 parts of angelica sinensis, 15-17 parts of asarum, 15-17 parts of cinnamon, 15-17 parts of flos chrysanthemi, 1000-1200 parts of rice wine, 200-300 parts of bitter melon juice, 2.3-2.5 parts of cellulase, 0.12-0.15 part of sodium bicarbonate, 0.5-0.6 part of casein and 30-35 parts of caramel. The traditional Chinese medicine formula based mild medicinal liquor for treating ozostomia has transparent color, elegant taste and smooth texture and contains rich nutrients.

The invention discloses sulfonium salt derivatives as well as a preparation method and medical application thereof, and particularly relates to a compound as shown in a formula I. The sulfonium salt derivatives with brand new structures are designed according to a bioisostere principle, and the compounds have good tolerance to gastric acid; compared with a natural product, the sulfonium salt compound provided by the invention has stronger alpha-glucosidase inhibitory activity, can be used as an onium salt type alpha-glucosidase inhibitor, and is mainly prepared from 1, 2-O-isopropylidene furan D-glucose as a raw material through a one-pot method. The onium salt derivative molecule with alpha-glucosidase inhibitory activity is obtained through multi-step reaction, and the molecule has hypoglycemic activity.

A preparation method and application of a highly active tetranuclear polymer. The invention discloses a tetranuclear copper compound containing difluorooxamide ligand and its preparation method and application. Dissolve the copper salt in 5-10ml of methanol and add it to the methanol solution of the above-mentioned mononuclear copper ligand, react at 60°C for 1 hour, then add the methanol solution containing 2mmol of 1,10-phenanthroline ligand to the above-mentioned ligand Reflux at 60°C for 5 hours, filter, and slowly volatilize the filtrate for 1 week to obtain blue blocky crystals. That is, it is a tetranuclear copper compound containing difluorooxamide ligand, and the copper salts are: copper perchlorate, copper chloride, copper bromide and the like. Compared with the platinum anti-cancer commonly used at present, the compound of the present invention has the characteristics of high anti-cancer activity, low cost, simple preparation method and the like. At the same time, it was found for the first time that the synthesized compound has a high activity in reducing blood sugar, which provides a new way for the development of drugs for related purposes.