A kind of Rosa roxburghii polysaccharide and its preparation method and application

A technology of prickly pear and polysaccharide is applied in the field of health food to achieve the effects of high purity, inhibitory activity and clear structure

Active Publication Date: 2020-06-19
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the regulation of intestinal microbiota and the promotion of intestinal health by Rosa roxburghii polysaccharides

Method used

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  • A kind of Rosa roxburghii polysaccharide and its preparation method and application
  • A kind of Rosa roxburghii polysaccharide and its preparation method and application
  • A kind of Rosa roxburghii polysaccharide and its preparation method and application

Examples

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Effect test

Embodiment 1

[0055] A preparation method of Rosa roxburghii polysaccharide, comprising the following steps:

[0056] (1) Raw material pretreatment: wash the fresh Rosa roxburghii fruit and put it in a tray, and dry it in a blast drying oven at 60°C for 48 hours. Crush, pass through a 40-mesh sieve, formulate a solid-to-liquid ratio of 1:6g / mL according to Rosa roxburghii dry powder and 95% ethanol (v / v), heat to reflux in a water bath at 70°C for 4 hours, filter to obtain the residue, heat to reflux and filter Repeat 4 times, put the degreased Rosa roxburghii dry powder into a blast drying oven at 45°C for 48 hours.

[0057] (2) Add 150 g of the treated Rosa roxburghii powder into distilled water for hot water leaching, the leaching temperature is 95° C., the time is 3 hours, and the ratio of solid to liquid is 1:30 (the units of mass and volume are g and mL, respectively). The number of times is 2 times; the centrifugal force is 5000g, centrifuged for 10min, and the supernatant is collec...

Embodiment 2

[0064] A preparation method of Rosa roxburghii polysaccharide, comprising the following steps:

[0065] (1) Raw material pretreatment: wash the fresh Rosa roxburghii fruit and put it in a tray, and dry it in a blast drying oven at 60°C for 48 hours. Grind, pass through a 50-mesh sieve, formulate a solid-to-liquid ratio of 1:5g / mL according to Rosa roxburghii dry powder and 95% ethanol (v / v), heat to reflux in a water bath at 75°C for 3 hours, filter to obtain the residue, heat to reflux and filter Repeat 3 times, put the degreased Rosa roxburghii dry powder into a blast drying oven at 55°C and dry for 36 hours.

[0066] (2) Get 150g of Rosa roxburghii dry powder treated in (1), add distilled water and carry out hot water extraction, extraction temperature 95 ℃, time 2h, solid-liquid ratio 1:20, extraction times is 3 times; Centrifugal force 8000g, After centrifugation for 5 min, the collected supernatant was concentrated to 1 / 3 of the original volume by rotary evaporation at ...

Embodiment 3

[0072] A preparation method of Rosa roxburghii polysaccharide, comprising the following steps:

[0073] (1) Raw material pretreatment: wash the fresh Rosa roxburghii fruit and put it in a tray, and dry it in a blast drying oven at 60°C for 48 hours. Grind, pass through a 60-mesh sieve, formulate a solid-to-liquid ratio of 1:4g / mL according to Rosa roxburghii dry powder and 95% ethanol (v / v), heat to reflux in a water bath at 80°C for 2 hours, filter to obtain the residue, heat to reflux and filter Repeat 2 times, put the degreased Rosa roxburghii dry powder into a blast drying oven at 65°C for 24 hours.

[0074] (2) Take 150g of Rosa roxburghii dry powder treated in (1), add distilled water for hot water extraction, extraction temperature is 95°C, time is 3.5h, solid-liquid ratio is 1:35, extraction times is 1 time; centrifugal force is 4000g , centrifuged for 15 min, and the supernatant was collected and concentrated to 1 / 5 of the original volume by rotary evaporation at 65°...

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Abstract

The invention discloses a rosa roxburghii polysaccharide, as well as a preparation method and application thereof. The preparation method mainly comprises raw material pretreatment, extraction, deproteinization, de-coloration, alcohol precipitation, as well as separation and purification, wherein the extraction is to mix pretreated rosa roxburghii dry powder and water in a mass volume ratio of (1to 15) to (1 to 35), and perform extraction at a temperature of 55 to 95 DEG C; perform centrifugal separation to obtain a rosa roxburghii polysaccharide extracting solution, and concentrate the rosaroxburghii polysaccharide extracting solution under reduced pressure to 1 / 3 to 1 / 6 of the original volume to obtain a concentrated solution of the rosa roxburghii polysaccharide. The rosa roxburghii polysaccharide prepared by the invention has the advantages of high purity and uniform molecular weight. Compared with the existing rosa roxburghii polysaccharide, as proved by in-vitro fermentation experiments, the rosa roxburghii polysaccharide disclosed by the invention can promote the generation of short-chain fatty acids such as acetic acid, propionic acid and butyric acid, promote the growthof beneficial bacteria such as lactic acid bacteria and bifidobacterium, and can be taken as prebiotics. Meanwhile, the rosa roxburghii polysaccharide can inhibit the activity of alpha-glucosidase, and can be applied to healthy foods or medicines having auxiliary therapy effects on intestinal diseases and diabetes mellitus.

Description

technical field [0001] The invention relates to Rosa roxburghii polysaccharide, in particular to Rosa roxburghii polysaccharide capable of regulating intestinal microecology and its preparation method and application, belonging to the field of health food. Background technique [0002] Prickly pear (Rose roxburghii Tratt), also known as Wenxianguo and Songchungui, is a deciduous shrub in the family Rosaceae. Its fruit is rich in nutrients such as flavonoids, polysaccharides, vitamins, superoxide dismutase and organic acids, and has high nutritional value. Rosa roxburghii, as a medicinal and edible plant with regional characteristics, is also eaten as a traditional Chinese medicine. Traditional medicine mainly uses Rosa roxburghii roots, leaves and fruits as medicines for digestion and spleen strengthening, diarrhea, and summer heat relief. It has a certain effect. Among them, "Compendium of Materia Medica Supplements" and "Qianshu" have detailed records of its pharmacologi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A23L33/105
CPCA23L33/105C08B37/0003
Inventor 扶雄汪磊李超黄强
Owner SOUTH CHINA UNIV OF TECH
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