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Process method for extracting stevia rebaudiana polyphenol from stevia rebaudiana

A process method and technology of stevia, applied in the directions of pharmaceutical formulations, non-central analgesics, medical preparations containing active ingredients, etc., can solve the problems of low purity of stevia polyphenols, complicated extraction process, low extraction rate, etc. , to achieve the effect of promoting economic development, huge economic benefits and simple operation

Inactive Publication Date: 2018-01-26
阜康市天鼎生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] So far, the existing extraction process of stevia polyphenols is not very mature, and there are still problems such as low purity of extracted stevia polyphenols, low extraction rate and complicated extraction process.
For example, the Chinese patent No. 201610745209.X discloses a steviol phenolic extract and its application in the preparation of antibacterial products. Therefore, a kind of technology of separately extracting stevia polyphenols from stevia rebaudiana does not appear yet

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] This embodiment discloses a process for extracting stevia polyphenols from stevia rebaudiana, which specifically includes the following extraction and separation process:

[0035] 1. Take fresh stevia to clean, weigh and beat with a beater to obtain a slurry;

[0036] 2. The slurry is placed in an aqueous acid solution with a pH value of 1 and left to stand for 2 hours, and the slurry after standing is filtered with 200 mesh gauze to obtain slag;

[0037] 3. Using ethanol as the extraction solution, the slag is subjected to ultrasonic extraction to obtain a crude extract; wherein, the conditions of the ultrasonic extraction are: the extraction solution is 35% ethanol, the number of ultrasonic waves is 3 times, and the ultrasonic temperature is 30°C. The ultrasonic power is 400W, and each ultrasonic time is 5min;

[0038] 4. Use supercritical carbon dioxide extraction technology to extract the crude extract to obtain a stevia polyphenol extract; wherein, the conditions ...

Embodiment 2

[0045] This embodiment discloses a process for extracting stevia polyphenols from stevia rebaudiana, which specifically includes the following extraction and separation process:

[0046] 1. Take fresh stevia to clean, weigh and beat with a beater to obtain a slurry;

[0047] 2. Place the slurry in an acid aqueous solution with a certain pH of 2 and let it stand for 3 hours, and use 200 mesh gauze to filter the standing slurry to obtain slag;

[0048] Step 3, using ethanol as the extraction solution, ultrasonically extracting the slag to obtain a crude extract; wherein, the conditions for ultrasonic extraction are: the extraction solution is 35% ethanol, the number of ultrasonic waves is 3 times, and the ultrasonic temperature is 40°C , ultrasonic power is 300W, each ultrasonic time is 2min;

[0049] Step 4, using supercritical carbon dioxide extraction technology to extract the crude extract to obtain a stevia polyphenol extract; wherein, the conditions for supercritical carb...

Embodiment 3

[0057] This embodiment discloses a process for extracting stevia polyphenols from stevia rebaudiana, which specifically includes the following extraction and separation process:

[0058] 1. Take fresh stevia to clean, weigh and beat with a beater to obtain a slurry;

[0059] 2. Place the slurry in an aqueous acid solution with a certain pH of 1.5 and let it stand for 2 hours, then use 200 mesh gauze to filter the left slurry to obtain slag;

[0060] 3. Using ethanol as the extraction solution, the slag is subjected to ultrasonic extraction to obtain a crude extract; wherein, the conditions of the ultrasonic extraction are: the extraction solution is 35% ethanol, the number of ultrasonic waves is 3 times, and the ultrasonic temperature is 36°C. The ultrasonic power is 340W, and each ultrasonic time is 5min;

[0061] 4. Use supercritical carbon dioxide extraction technology to extract the crude extract to obtain a stevia polyphenol extract; wherein, the conditions for supercrit...

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Abstract

The invention discloses a process method for extracting stevia rebaudiana polyphenol from stevia rebaudiana. The process method comprises the following steps: washing fresh stevia rebaudiana, weighing, and pulping with a beater to obtain slurry; standing the slurry in an acid solution having a certain pH value for a period, filtering with gauze to obtain slag; performing ultrasonic extraction on the slag by using alcohol as an extracting liquid to obtain crude extract; extracting the crude extract by using supercritical carbon dioxide extraction technology to obtain a stevia rebaudiana polyphenol extract; and purifying the stevia rebaudiana polyphenol extract to obtain stevia rebaudiana polyphenol powder. The process method can be used for singly and specifically extracting stevia rebaudiana polyphenol from stevia rebaudiana, has relatively high extracting purity and extraction rate, is simple in operation, and is suitable for industrial production for stevia rebaudiana polyphenol.

Description

technical field [0001] The invention relates to the technical field of extraction of plant extracts, more specifically, the invention relates to a process for extracting stevia polyphenols from stevia rebaudiana. Background technique [0002] Stevia is a perennial herb of the Asteraceae family, native to Paraguay and Brazil in South America. It is one of the sugar family plants known to have higher sweetness, and has become the third natural sugar source after caramel and beet sugar. At present, China is the world's largest producer and supplier of steviol glycosides, accounting for more than 80% of the global total. Stevia is rich in steviol glycosides, stevia polyphenols, flavonoids, chlorogenic acid and other components, and these components have important biological activities. Studies have shown that stevia polyphenols have hypoglycemic activity, anti-inflammatory activity and antibacterial activity at the same time, and have great medicinal value. [0003] So far, th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61P3/10A61P29/00A61P31/04
Inventor 钱金金陈建国汪晶
Owner 阜康市天鼎生物科技有限公司
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