Angelica dahurica coumarin extract as well as preparation method and quality control method thereof

A technology of coumarin and extract, applied in the field of Angelica dahurica coumarin extract and quality control, can solve the problems of inability to accurately judge the quality of effective parts, complex components, etc., and achieve the effects of clear drug effect, high content and stable quality

Inactive Publication Date: 2010-02-03
CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the complex ingredients contained in the effective part of natural medicines, the quality of the effective part cannot be accurately judged only by a few index components.

Method used

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  • Angelica dahurica coumarin extract as well as preparation method and quality control method thereof
  • Angelica dahurica coumarin extract as well as preparation method and quality control method thereof
  • Angelica dahurica coumarin extract as well as preparation method and quality control method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation of embodiment 1 Angelica dahurica coumarin of the present invention

[0025] Weigh 5000g of Angelica dahurica crude powder (20 mesh), 10 parts in total, add 8 times the amount of 75% ethanol to reflux and extract 3 times, each time for 3 hours, filter, combine the extracts, reclaim the ethanol under reduced pressure, and dissolve the medicinal solution Concentrate under reduced pressure until the concentration is equivalent to 2g of medicinal materials per ml, let it cool, add 2 times the amount of petroleum ether to extract once, discard the petroleum ether layer (that is, the extraction phase), and add ethyl acetate to the raffinate phase to extract 3 times Each time, 2 times the amount of ethyl acetate was added each time, the extract phases were combined, the ethyl acetate was recovered under reduced pressure, and the extract was concentrated and dried in vacuum to obtain the coumarin extract of Dahurian Angelica dahurica.

[0026] Since the total co...

Embodiment 2

[0027] The extraction process screening test of embodiment 2 Radix Angelica dahurica coumarin

[0028] 1. Instruments and materials

[0029] 1.1 Instrument

[0030] 752 type ultraviolet spectrometer (Shanghai Spectrum Instrument Co., Ltd.); IS09001 electronic balance (Beijing Sartorius Balance Co., Ltd.); KQ-100DE type numerical control ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); HA121-50-01 type Supercritical CO 2 Extraction device (Jiangsu Huaan Supercritical Extraction Co., Ltd.); RE-52A rotary evaporator (Shanghai Yarong Biochemical Instrument Factory); SHB-3 circulating vacuum pump (Zhengzhou Dufu Instrument Factory); HH-S digital constant temperature water bath (Jiangsu Jintan City Medical Instrument Factory); SL500 stainless steel percolation tank (Hengyang Jinshan Traditional Chinese Medicine Equipment Manufacturing Co., Ltd.); DHG-9070A electric heating constant temperature blast drying oven (Shanghai Qixin Instrument Co., Ltd.).

[0031] 1.2 Mate...

Embodiment 3

[0094] The condition parameter selection experiment of embodiment 3 Chuan Baizhi coumarin separation and purification process

[0095] 1 Instruments and materials

[0096] 1.1 Instrument

[0097] Model 752 UV Spectrometer (Shanghai Spectrum Instrument Co., Ltd.); RE-52A Rotary Evaporator (Shanghai Yarong Biochemical Instrument Factory); SHB-3 Circulating Vacuum Pump (Zhengzhou Dufu Instrument Factory); HH-S Digital Display Constant Temperature Water Bath (Jiangsu Jintan Medical Instrument Factory); ISO9001 electronic balance (Beijing Sartorius Balance Co., Ltd.); Centrifuge refrigerated centrifuge (Germany Eppendorf); PHs-2c acidity meter (Chengdu Ark Technology Development Company)

[0098] 1.2 Materials

[0099] Chloroform, cyclohexane, diethyl ether, ethyl acetate, petroleum ether, hydrochloric acid, sodium chloride, sodium chloride carbonate and sodium chloride phosphate are all analytically pure (Chengdu Kelong Reagent Factory)

[0100] 2. Experimental methods and resu...

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Abstract

The invention provides an angelica dahurica coumarin extract, wherein the total content of coumarin is not lower than 44.672mg / g, and the content of imperatorin is not lower than 68.623mg / g. The invention further provides an extraction method and a quality control method of the extract. The angelica dahurica coumarin extract has high extraction purity, high content of coumatin, stable quality andfunction of 5-hydroxytryptamine regulation, is capable of treating diseases caused by 5-hydroxytryptamine besides migraine, has distinguished efficacy and strong pertinency of indications, and provides a new choice for clinical treatment.

Description

technical field [0001] The invention relates to an extract of coumarin from Angelica dahurica and a quality control method, belonging to the field of medicines. Background technique [0002] Angelica dahurica is a commonly used traditional Chinese medicine, which has the effects of dispelling wind and cold, drying dampness and discharging pus, and is often used to treat wind-cold colds, nasal sinusitis headaches and other symptoms. The Angelica dahurica (Fisch.ex Hoffm.) Benth.et Hook.f. or Hang Baizhi A.dahurica (Fisch.ex Hoffm.) Benth.etHook.f recorded in the 2000 edition of "Chinese Pharmacopoeia" is Umbelliferae Angelica dahurica (Fisch.ex Hoffm.) Benth.etHook.f .var.formosana (Boiss.) Dried root of Shan et Yuan. The active ingredients contained are mainly coumarin compounds such as oxidized precurol, imperatorin and isoimperatorin. Angelica dahurica coumarin has anti-inflammatory, inhibiting the production of prostaglandin E2, antihistamine effect, photosensitization ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/232A61P25/06A61P25/00C07D493/04
Inventor 李祖伦李玲秦旭华
Owner CHENGDU UNIV OF TRADITIONAL CHINESE MEDICINE
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