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Method for using subcritical water to extract grifolan

The technology of Grifola frondosa polysaccharide and subcritical water is applied in the field of extraction technology of Grifola frondosa polysaccharide, which can solve the problems of high production cost, complex components, difficult separation and purification procedures, etc., and achieves low equipment cost and operating cost, and extraction efficiency. And the effect of improving the purity and reducing the waste of raw materials

Inactive Publication Date: 2017-03-08
JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The enzymatic hydrolysis extraction method has the advantages of high polysaccharide extraction rate, mild reaction conditions, and little damage to the polysaccharide structure. However, due to the high price of enzymes and relatively high production costs, it is not suitable for large-scale industrial production.
Ultrasonic extraction and microwave extraction can increase the extraction rate of polysaccharides and shorten the extraction time, but sometimes the components of the polysaccharide extract are more complex, which brings certain difficulties to the subsequent separation and purification process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A method for subcritical water extraction of Grifola frondosa polysaccharides, comprising the following steps:

[0025] (1) Drying: Take the fruiting bodies of Grifola frondosa and dry them twice. The first drying temperature is controlled at 75°C and the drying time is 6 minutes to remove most of the water in the fruiting bodies of Grifola frondosa. The drying temperature is controlled at 57°C, and the drying time is 13 minutes, and the remaining water in the fruiting body of Grifola frondosa is further slowly removed until the moisture in the fruiting body of Grifola frondosa is reduced to 5%~8%, and the dried fruiting body of Grifola frondosa is obtained;

[0026] (2) Pulverization: use a high-speed universal pulverizer to pulverize the dry fruiting body of Grifola frondosa, pass through 55 sieves, and obtain dry fruiting body powder of Grifola frondosa;

[0027] (3) Subcritical extraction: Put the dry fruiting body powder of Grifola frondosa and deionized water into...

Embodiment 2

[0040] A method for subcritical water extraction of Grifola frondosa polysaccharides, comprising the following steps:

[0041] (1) Drying: Take the fruiting bodies of Grifola frondosa and dry them twice. The first drying temperature is controlled at 76°C and the drying time is 6 minutes to remove most of the water in the fruiting bodies of Grifola frondosa. The drying temperature is controlled at 61°C, and the drying time is 14 minutes, and the remaining water in the fruiting body of Grifola frondosa is further slowly removed until the water in the fruiting body of Grifola frondosa is reduced to 5%~8%, and the dried fruiting body of Grifola frondosa is obtained;

[0042] (2) Pulverization: use a high-speed universal pulverizer to pulverize the dry fruiting body of Grifola frondosa, pass through a 45-90 μm sieve, and obtain dry fruiting body powder of Grifola frondosa;

[0043](3) Subcritical extraction: Put the dry fruiting body powder of Grifola frondosa and deionized water i...

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PUM

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Abstract

The invention discloses a method for using subcritical water to extract grifolan. The method comprise the steps of stoving, smashing, subcritical extraction, rough filtration, heat preservation, centrifugation, concentration, purification, decolorization, precipitation, drying, re-washing, drying, secondary extraction and the like. The method specifically comprises the steps of taking advantages of good permeability and solubility of the subcritical water to make cell wall polysaccharide be in full contact with the subcritical water so that the cell wall polysaccharide can be fully dissolved out, conducting concentration on the cell wall polysaccharide through a water bath method, conducting purification on the grifolan by combining an Sevag method, and at the same time recycling a first centrifugation precipitation which can be used for conducting the secondary extraction on the grifolan. In this way, not only extraction content of the grifolan is increased, waste in raw material is also sharply reduced. Compared with existing other extraction methods, both the equipment cost and the operation cost according to the method for using the subcritical water to extract the grifolan are relatively lower, and extraction efficiency and purity of the grifolan are obviously improved.

Description

technical field [0001] The invention relates to a process for extracting the polysaccharide from Grifola frondosa, in particular to a method for extracting the polysaccharide from Grifola frondosa with subcritical water. Background technique [0002] Grifola frondosa (Maitake) polysaccharide is an extract from Grifola frondosa, which is composed of dextran, glucose, xylose, fucose, xylose, mannose, lactose and a small amount of heteroglycan complexes of protein composition. Grifola frondosa polysaccharide contains a kind of proteoglycan, which is called Maitake D-fraction in Chinese. The ratio of protein to polysaccharide is 7:3, and the average molecular weight is 1 million. The polysaccharide fraction consists of β-(1-6) glucans with β-(1-3) side chains and β-(1-3) glucans with β-(1-6) side chains. Among the polysaccharide ingredients, only this kind of maitake mushroom has the most anti-cancer and anti-cancer effects. [0003] In recent years, researchers have used eme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/006C08B37/0003
Inventor 邵兴军毛日文朱惠芬仲延龙
Owner JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECH
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