Immune cell tumor killing capability detection model and construction method and application thereof

A technology of immune cells and detection models, applied in the field of medicine, can solve the problems of low income for patients with advanced tumors, and achieve the effect of short modeling cycle, cheap price and simple construction method

Active Publication Date: 2019-11-05
义慧科技(深圳)有限公司
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  • Application Information

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Problems solved by technology

Although traditional treatment options, such as surgery, radiotherapy and chemotherapy, can improve the prognosis of patients to a certain extent, surgery has little

Method used

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  • Immune cell tumor killing capability detection model and construction method and application thereof
  • Immune cell tumor killing capability detection model and construction method and application thereof

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Embodiment 1

[0038] figure 1 The flow chart of the construction method for the detection model of the immune cell killing tumor ability provided by the embodiment of the present invention, combined with figure 1 A method for constructing a detection model for the ability of immune cells to kill tumors provided in this embodiment comprises the following steps:

[0039] (1) Construction of zebrafish tumor blood supply metastasis model:

[0040] a. Preparation of fluorescently labeled tumor cells

[0041] All kinds of primary tumor cells are suitable for this model, and melanoma cells are used as a specific tumor cell example to introduce here. Dilute the DMSO solution containing the living cell stain Calcein-AM with PBS buffer (phosphate buffer) at pH=7.4 to make a Calcein-AM solution with a concentration of 10 μM, and add 1 mL of the Calcein-AM solution to the Substitute melanoma cells in a 10 cm dish. Incubate the melanoma cells at 37°C for 30 minutes, then wash the cells twice with ro...

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Abstract

The invention provides a construction method of an immune cell tumor killing capacity detection model. The construction method comprises the following steps: (1) 24 after egg membranes of embryos of zebrafish fertilized for 24-96 hours are removed, injecting tumor cells labeled by a first living cell staining agent into zebrafish bodies, then executing culturing in culture water containing a melanin inhibitor, and screening out the zebrafish with the tail parts labeled by the first living cell staining agent; (2) after the collected zebrafish is anesthetized, the zebrafish is divided into an immune cell injection group and a control group, injecting immune cells labeled by a second living cell staining agent into the immune cell injection group in a microinjection mode, injecting a carriersolution into the control group, and then putting the zebrafish in the culture water containing the melanin inhibitor for continuous culture. Short period, time saving, labor saving and low modelingcost are achieved by adopting the construction method, and a constructed detection model can accurately and objectively evaluate the effect of tumor immunotherapy. The invention also provides a detection model prepared based on the method and application thereof.

Description

technical field [0001] The invention relates to the field of medicine, in particular to a detection model for the ability of immune cells to kill tumors and its construction method and application. Background technique [0002] Malignant tumors have become the culprit that threatens human life, and the incidence rate is on the rise. Although traditional treatment options, such as surgery, radiotherapy and chemotherapy, can improve the prognosis of patients to a certain extent, surgery has little benefit for patients with advanced tumors, and the huge side effects of radiotherapy and chemotherapy further limit its wide application. Therefore, finding new tumor treatment methods has always been a hot research direction. Immunotherapy has made remarkable progress in the field of tumor treatment in recent years, and it can be said to be a great hope for patients who cannot tolerate surgery, are insensitive to radiotherapy and chemotherapy, and are intolerant. [0003] Immunoth...

Claims

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/10Y02A40/81
Inventor 杨云龙曹义海
Owner 义慧科技(深圳)有限公司
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