Application of ovatodiolide to preparation of composition for restraining protein synthesis of helicobacter pylori
A technology of Helicobacter pylori and hinolide, which is applied in the field of inhibiting drug-resistant gastric Helicobacter pylori, can solve the problems of unclear inhibition mechanism, easy interaction, and complicated use of drugs
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0022] Example 1: Minimal Inhibitory Concentration of Diogenolide against Helicobacter pylori
[0023]Different concentrations of ovatodiolide (ovatodiolide) paper ingots were used to culture Helicobacter pylori for two days, and the minimum concentration of ovatodiolide to inhibit Helicobacter pylori was determined by the agar-well diffusion method. Based on this analysis, the minimum concentration of hinolide inhibiting the growth of the standard Helicobacter pylori strain (H. pylori 26695) was analyzed. Table 1 shows the minimum inhibitory concentration for inhibiting the standard strain of Helicobacter pylori (H. pylori 26695). From the experimental results, it can be seen that 10μM and 20μM hurgeonides have inhibition rings, and the diameters of the inhibition rings are 10±0.5mm and 19±0mm, which proves that the minimum concentration of h. pylori inhibited by h. pylori is 10 μM.
[0024] Table 1. Minimum inhibitory concentration for inhibiting Helicobacter pylori standar...
Embodiment 2
[0026] Embodiment 2: the minimum bactericidal concentration of anti-helicobacter pylori standard strain and its multi-drug resistant bacterial strain
[0027] Different concentrations of hinolide were prepared by serial dilution, and cultured with the standard strain of Helicobacter pylori isolated from the laboratory and multi-drug resistant Helicobacter pylori (v633, v1354) for six hours, and then spread the plate method (spread plate method) Observe and analyze the number of colony growth, and analyze the minimum bactericidal concentration of hinolide that can completely kill standard strains of Helicobacter pylori and multi-drug resistant strains. It can be seen from the results in Table 2 that the minimum bactericidal concentration of hinolide against the standard strain of Helicobacter pylori is 200 μM, while the minimum bactericidal concentration against multi-drug resistant strains v633 and v1354 is 100 μM.
[0028] Table 2. Minimal bactericidal concentration of hinoli...
Embodiment 3
[0030] Example 3: Diogenolide inhibits the protein synthesis of Helicobacter pylori
[0031] Since the structure of deer needle lactone is similar to that of antibiotics that inhibit protein production, in order to understand whether the mechanism of deer needle lactone inhibiting Helicobacter pylori is related to protein synthesis, an in vitro transcription-translation system (in vitroTranscription / Translation system) was used , after 10 minutes of the action of fertilin and Escherichia coli extract (E.coli S30), pGL3 with luciferase (luciferase) was added for transcription and translation reaction for 2 hours, and finally the dual luciferase reporter gene was used to Detection system (Dual-Luciferase Reporter Assay System), add luciferin to the finished sample to make it color, and then measure the absorbance value with a microplate luminometer, and you can know the cryolide Whether it inhibits protein synthesis.
[0032] Among them, kanamycin (kanamycin) inhibiting 30S sub...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More