Model mouse preparation method and recombinant vector for conditional cell removal

A recombinant vector and model mouse technology, applied in the direction of recombinant DNA technology, the introduction of foreign genetic material using a vector, and the cells modified by the introduction of foreign genetic material, etc. Scientific research and practical applications, etc., to achieve accurate and intuitive experimental results and shorten the time.
CN103409448AActive Publication Date: 2013-11-27BIOCYTOGEN PHARMACEUTICALS (BEIJING) CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
BIOCYTOGEN PHARMACEUTICALS (BEIJING) CO LTD
Publication Date
2013-11-27

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Abstract

The invention discloses a recombinant vector for conditional cell removal and a preparation method thereof. The recombinant vector comprises a DNA (Deoxyribonucleic Acid) fragment structure iDTR-2A-FP, wherein iDTR is a coding gene of a diphtheria toxin receptor, 2A can realize a coded nucleic acid sequence of a sheared polypeptide fragment through ribosome jumping, and FP is a coding gene of fluorescent protein. The invention further provides a method for preparing a model mouse for conditional cell removal. A descendant mouse, obtained after the model mouse for cell removal, prepared by the method provided by the invention, is mated with a Cre mouse, has the following characteristics: through expression conditions of the fluorescent protein, direct detection on cell removal efficiency can be carried out, and whether cells are completely removed from in vivo or not can be determined, so that more accurate and intuitive results can be obtained; and the expression or deletion of target genes can be controlled from time through controlling the inducer giving time.
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Description

technical field

[0001] The invention relates to the field of biotechnology, in particular to a recombinant vector for conditional cell elimination, a preparation method thereof, and a method for establishing a conditional cell elimination model mouse Background technique

[0002] Conditional cell deletion is based on the chromosomal site-specific recombinase system, and the commonly used site-specific recombinase systems are Cre-LoxP and Flp-FRT systems. For example, a pair of LoxP-Stop-LoxP sequences is placed upstream of the DNA sequence of a conditional lethal gene to be knocked in to obtain a conditional cell knockout model mouse. Then, the conditional cell knockout model mice were bred with the Cre mice with cell-specific expression to obtain mice with conditional lethal gene knock-in in specific cells, that is, to obtain conditional gene knock-in mice. Then, the specific cells can be knocked out by controlling the administration time of the inducer.

[0003] Diphther...

Claims

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