Method for extracting ovatodiolide and anisomeles indica lactonic acid
An extraction method, the technology of parsnips, which is applied in the field of extraction and separation of traditional Chinese medicine, can solve the problems of no purification method of parsnip lactone acid, etc., achieve good application prospects, improve utilization rate, and reduce extraction costs
Active Publication Date: 2021-08-13
甘肃润达药业有限公司
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[0005] But there is no relevant patent report on the purification method of parsnip lactone at present, so a kind of extraction method of parsnip lactone is proposed to improve the effect of parsnip lactone The research of and parsnip lactone acid is of great significance in the field of "anti-cancer stem cell medicinal chemistry"
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Embodiment 1
[0033] A method for extracting parsniplide and parsniplide acid, comprising the steps of:
[0034] S1: Selection of samples to be tested: specifically include the following steps:
[0035] S101: Raw material selection and processing: Classify unflowered leaves, flowering roots, unflowered roots, flowering stems, unflowering stems, flowering leaves, and samples from Dapu County, weigh 1g of the samples of Parsnips to be tested in the above classifications, and use After soaking and cooling in liquid nitrogen for 2 minutes, quickly grind the sample to be tested to powder, put the powder into a 5mL centrifuge tube, add 10mL ethanol solution to the centrifuge tube and soak for 4 hours;
[0036] S102: filter the soaking solution: filter the soaking solution in the centrifuge tube, and take the supernatant into the test tube;
[0037] S103: Preparation of the solution to be tested: Add activated carbon powder into the test tube, let it stand for 10 minutes, let the solution fully d...
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The invention discloses a method for extracting ovatodiolide and anisomeles indica lactonic acid, and belongs to the technical field of traditional Chinese medicine extraction and separation. The method comprises the following steps: (1) classifying and selecting an anisomeles indica sample to be detected, crushing the selected anisomeles indica sample, and soaking the crushed anisomeles indica sample in an extraction solvent; (2) carrying out suction filtration and washing on the anisomeles indica soaking solution, and evaporating and concentrating a mother solution obtained by suction filtration; (3) injecting the concentrated solution into a chromatographic column for elution and separation, and performing column chromatography twice to obtain crude ovatodiolide and crude anisomeles indica lactonic acid; and (4) recrystallizing the crude ovatodiolide and the crude anisomeles indica lactonic acid at a low temperature to obtain pure ovatodiolide and pure anisomeles indica lactonic acid. According to the method, the ovatodiolide and the anisomeles indica lactonic acid can be extracted at the same time, the utilization rate of anisomeles indica raw materials is effectively improved, the extraction cost is reduced, the purity and yield of the obtained products are high, the product quality is effectively guaranteed, and the method has a good application prospect.
Description
technical field [0001] The invention belongs to the technical field of extraction and separation of traditional Chinese medicines, and in particular relates to a method for extracting parsnipolactone and parsniprolactone acid. Background technique [0002] Parsnip, also known as Hindu, is the whole herb of the plant Parsnip in the family Lamiaceae, and is a common erect herbal medicinal plant in southwest, southeast, south of the Yangtze River and southeast Tibet. As early as 1711 in the Qing Dynasty, the book "Preparing Herbal Medicine by Raw Herbal Medicine" recorded its medicinal value, and it was often used for colds, body heat, vomiting, abdominal pain, muscle and bone pain and other diseases. Ovatodiolide is an important active component of parsnips. In recent years, there have been more and more pharmacological studies on parsnidin, and many research papers have reported that parsnid has selective killing effect on various cancer stem cells. [0003] At present, res...
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IPC IPC(8): C07D493/08
CPCC07D493/08
Inventor 杨震曾耀铭常青曾纪锴
Owner 甘肃润达药业有限公司