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Octopus oligopeptide capable of promoting synthesis of breast casein and preparation method and application of octopus oligopeptide

A casein and lactation-promoting technology is applied in the field of octopus oligopeptide for promoting the synthesis of breast casein and its preparation, and achieves the effects of good development value, improved expression level and growth promotion.

Active Publication Date: 2019-11-05
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no relevant reports on the extraction and separation of oligopeptides from octopus and their application in the development of postpartum functional foods

Method used

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  • Octopus oligopeptide capable of promoting synthesis of breast casein and preparation method and application of octopus oligopeptide
  • Octopus oligopeptide capable of promoting synthesis of breast casein and preparation method and application of octopus oligopeptide
  • Octopus oligopeptide capable of promoting synthesis of breast casein and preparation method and application of octopus oligopeptide

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Experimental program
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Effect test

Embodiment 1

[0026] Preparation of octopus oligopeptide: Wash the fresh octopus, cut into small pieces and grind it with a meat grinder, add deionized water according to the ratio of material to liquid 1g / 2mL, add neutral protease 3000 U / g for enzymatic hydrolysis, pH The value is 6.5, shaken in a constant temperature water bath at 55°C for 5 hours, inactivating enzymes in a boiling water bath for 10 minutes, centrifuging at 10000r / min at 4°C for 30 minutes at a low temperature, taking the supernatant, ultrafiltering the supernatant with a 5kDa ultrafiltration membrane, taking the filtrate to concentrate, and then using The macroporous adsorption resin DA201-C is desalted, concentrated, separated by Sephadex G-25, and eluted with distilled water. The separation spectrum is as follows: figure 1 As shown, the first group of peak samples (OH1) were enriched, concentrated, and freeze-dried to obtain octopus oligopeptides.

[0027] Correspondingly, in the above steps, the macroporous adsorption...

Embodiment 2

[0029] Preparation of octopus oligopeptides: Wash fresh octopus, cut into small pieces and use a meat grinder to homogenate and grind, add deionized water at a ratio of 1g / 3mL to liquid, adjust the pH value to 8.5 with NaOH, and add alkaline protease 2000 U / g enzymatic hydrolysis, 60°C constant temperature water bath shaking for 6h, boiling water bath to inactivate enzyme for 10min, 10000r / min, 4°C low temperature centrifugation for 30min, take the supernatant, ultrafilter the supernatant with a 5kDa ultrafiltration membrane, take the filtrate and concentrate , then desalted with macroporous adsorption resin DA201-C, concentrated, separated with Sephadex G-25, eluted with distilled water, enriched the first group of peak samples (OH1), concentrated, and freeze-dried to obtain octopus oligopeptides.

Embodiment 3

[0031] Preparation of octopus oligopeptides: Wash fresh octopus, cut into small pieces and use a meat grinder to homogenate and grind, add deionized water according to the ratio of material to liquid 1g / 4mL, adjust the pH value to 8.0 with NaOH, add alkaline protease 4000 U / g enzymatic hydrolysis, shaking in a constant temperature water bath at 50°C for 4h, inactivating the enzyme in a boiling water bath for 10min, centrifuging at 10000r / min at 4°C for 30min at low temperature, taking the supernatant, ultrafiltering the supernatant with a 5kDa ultrafiltration membrane, and filtering the filtrate Concentrate, then desalt with macroporous adsorption resin DA201-C, concentrate, then separate with Sephadex G-25, elute with distilled water, enrich the first group of peak samples (OH1), concentrate, freeze-dry to obtain octopus oligopeptide .

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Abstract

The invention discloses an octopus oligopeptide capable of promoting synthesis of breast casein and a preparation method and application of the octopus oligopeptide. The octopus oligopeptide is prepared from one or more than two of the following amino acid sequences of MGDVLNF, MGLAGPR, EAPLMHV and TEAPLMHV. According to the octopus oligopeptide, the growth of HC11 in mammary epithelial cells canbe significantly promoted, the expression level of beta-casein of the HC11 in the mammary epithelial cells is increased, and compared with a blank control group, the expression level is increased by 150% or above. The results indicate that the octopus oligopeptide has good application prospects in functional nutritious foods capable of promoting lactation and improving the milk quality on postpartum women.

Description

technical field [0001] The invention belongs to the field of functional nutritious food, and in particular relates to an octopus oligopeptide for promoting the synthesis of mammary gland casein, a preparation method and application thereof. Background technique [0002] Breast milk is the most ideal natural food for babies after birth. Breast feeding has advantages that formula milk substitutes cannot match in terms of nutrition and immunology. The Global Strategy on Infant and Young Child Feeding jointly formulated by the World Health Organization and the United Nations Children's Fund clearly states that infants should be exclusively breastfed for the first 6 months. However, the United Nations Children's Fund survey shows that the exclusive breastfeeding rate of infants under 6 months of age in my country was only 28% in 2012-2014, and it has not increased within 3 years. There is still a big gap in the target. Delayed lactation and insufficient lactation in postpartum w...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/22C07K1/16A23L33/18
CPCA23V2002/00A23L33/18C07K7/06C12P21/06A23V2200/30A23V2250/55
Inventor 蔡冰娜潘剑宇孙恢礼陈华万鹏
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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