Construction method and application of peripheral blood B cell lines

A construction method and peripheral blood technology, applied in the field of construction of peripheral blood B cell lines, can solve the problems of lack of research and application of inflammatory diseases
CN110423723APending Publication Date: 2019-11-08NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
Publication Date
2019-11-08

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Abstract

The invention discloses a construction method and application of peripheral blood B cell lines (BCL). The BCL constructed in the invention have the characteristic of immortalized growth, the proportion of CD19<+> cells in the BCL is increased, the expression levels of CD138, CD38 and CD27 in the CD19<+> BCL are significantly higher than that of CD19<+> B cells before Epstein-Barr virus (EBV) transformation, and the capacities of antibody secretion and antigen presentation of the B cells are retained. The BCL disclosed by the invention can be used for screening dominant T cell immune response epitopes, or preparing a high-titer anti-HBsAg monoclonal antibody and inducing an HBV specific T cell line, and providing a new direction for removing hepatitis B.
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Description

technical field

[0001] The invention relates to a method for constructing a peripheral blood B cell line and its application, in particular to a method for constructing a peripheral blood B cell line capable of presenting HBV antigen peptides and its application. Background technique

[0002] According to the Global Burden of Disease Study in 2013, hepatitis virus infection is the seventh leading cause of death in the world, of which hepatitis B virus (Hepatitis B virus, HBV) and hepatitis C virus infection accounted for 96% of deaths [1]. In HBV infection, the body's immunity against HBV can be divided into two parts, one is the innate immune response, which is the non-specific immune response of the immune system; the other is the adaptive immune response, which is the specificity of T cells and B cells against HBV antigens immune response. In chronic HBV infection, the functional state of HBV-specific T cells is closely related to the clearance of the virus. Among them,...

Claims

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