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Macro protein extraction method suitable for environmental samples and fecal samples

An extraction method and macro-protein technology, which can be used in peptide preparation methods, chemical instruments and methods, organic chemistry, etc., can solve the problems of protein loss in processing steps, difficulty in protein extraction, etc., and achieve good purity, clear and abundant bands, and protein. high yield effect

Inactive Publication Date: 2019-11-12
SHANGHAI MAJORBIO BIO PHARM TECH
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Problems solved by technology

[0006] The technical problem mainly solved by the present invention is that it is difficult to extract protein from samples collected in the natural environment because they contain many interfering substances, and the processing steps will cause further loss of protein. protein extraction method

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  • Macro protein extraction method suitable for environmental samples and fecal samples
  • Macro protein extraction method suitable for environmental samples and fecal samples
  • Macro protein extraction method suitable for environmental samples and fecal samples

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Embodiment 1

[0066] A macroprotein extraction method suitable for environmental samples and stool samples, comprising the following steps:

[0067] (1) preparation of extraction buffer;

[0068] (2) Weigh the sample and transfer it to the tissue grinding tube, add the extraction buffer prepared in step (1) according to the mass ratio of 1:3 and mix it, move it to the MP tissue grinder, grind it at a speed of 6m / s for 30s, Move it to ice and keep it for 5 minutes, and use it as a grinding process, carry out this grinding process three times, and get the regrind material. The present invention uses a high-throughput tissue grinder to wet grind the sample. Compared with using the NoviPure extraction kit, it saves costs;

[0069] (3) Place the regrind material on ice, and crack it for 30 minutes to obtain the cracked material. After wet grinding with a high-throughput tissue grinder in the present invention, place it on ice for 30 minutes, and finally obtain clearer and richer bands;

[0070...

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Abstract

The invention discloses a macro protein extraction method suitable for environmental samples and fecal samples, and belongs to the field of protein extraction. According to the invention, the whole process of protein extraction is optimized, and the method comprises the following processes: carrying out wet grinding on a sample by using a high-flux tissue grinding instrument, carrying out proteinextraction on the sample by using a self-prepared extracting solution, placing the sample on ice for splitting decomposition after the wet grinding on the sample. The macro protein extraction method suitable for environmental samples and fecal samples is established, most of environmental samples and fecal samples can be successfully extracted, the extracted protein has higher yield, better purityand more clear and rich bands, and the method has obvious advantages for subsequent mass spectrometry identification. The invention solves the problem that: the protein is difficult to extract due tothe fact that the samples collected in the natural environment contain a plurality of interfering substances at present, and the further loss of the protein can be caused by the processing steps.

Description

technical field [0001] The invention relates to the field of protein extraction, in particular to a macro-protein extraction method suitable for environmental samples and stool samples. Background technique [0002] Due to the low concentration of components to be measured in environmental samples, complex components, many interfering substances, and obvious environmental influences, complex extraction, purification, concentration and other processing techniques are usually required to determine the components to be measured. Environmental samples are usually divided into water samples, soil and sediment, atmospheric samples and biological samples according to their form. The research significance of environmental samples is reflected in the development of functional biological indicators, tracking new functional genes and complex metabolic pathways, and re-examining the concept of microbial ecology from a functional perspective. With the impact of human activities, the imp...

Claims

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Application Information

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IPC IPC(8): C07K1/14C07K1/30
CPCC07K1/145C07K1/30
Inventor 冯景山冯利兴杨兵韩继臣
Owner SHANGHAI MAJORBIO BIO PHARM TECH
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