Full-extraction molecular diagnosis microfluidic chip and microfluidic system

Abstract

The invention relates to a full-extraction molecular diagnosis microfluidic chip and a microfluidic system. The full-extraction molecular diagnosis microfluidic chip includes a storage cavity, a sample cavity, a first liquid adding cavity, a first buffer cavity, at least one controlled liquid adding cavity, a second liquid adding cavity, a distribution cavity, a first liquid waste cavity, a buffering cavity, a second liquid waste cavity, at least one measuring cavity and at least one PCR amplification cavity. The portions, near the rotating center of a target, of the controlled liquid adding cavities are communicated with the second liquid adding cavity through air pipelines, the air pipelines are internally provided with phase change valves, and the controlled liquid adding cavities are communicated with the distribution cavity through liquid adding pipelines. Sample splitting, nucleic acid purification, equal distribution of reagents, amplification of nucleic acids in the chambers under restrictions of specific primers and other processes are all integrated in the centrifugal microfluidic chip, subsequent analysis work such as fluorescence signal collection and analysis can be achieved, a single-sample multi-index diagnosis mode is achieved, and it is possible to screen multiple pathogens of diseases through molecular diagnosis.

Description

technical field [0001] The present application relates to the field of centrifugal microfluidics, in particular to a microfluidic chip and a microfluidic system for molecular diagnosis of whole extraction. Background technique [0002] Microfluidics refers to the manipulation of liquids on the submillimeter scale. It integrates the basic operating units involved in the fields of biology and chemistry, and even integrates the functions of the entire laboratory, including sampling, dilution, reaction, separation, detection, etc., on a small chip, so it is also called Lab-on-a-Chip (Lab-on-a-Chip). -a-Chip). This kind of chip is generally composed of various liquid storage pools and interconnected microchannel networks, which can greatly shorten the sample processing time, and realize the maximum utilization efficiency of reagent consumables through precise control of liquid flow. Microfluidics provides extremely broad prospects for applications in many fields such as biomedi...

AI Technical Summary

Problems solved by technology

[0007] Based on this, it is necessary to provide a fully extracted molecular diagnostic microfluidic chip and a microfluidic system, which mainly solves the problem of how to integrate fully extracted molecular diagnostics into a centrifugal microfluidic chip, which can lyse samples, purify nucleic acids, and The entire process of amplification under the constraints of specific primers is integrated in a centrifugal microfluidic chip, and thus the collection and analysis of fluorescent signals are realized.

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