Temperature control device and method for nucleic acid reaction

Abstract

The invention discloses a temperature control device and method used for biomolecules, especially research in the nucleic acid reaction field. The device comprises multiple levels of temperature control units, and each level of temperature control unit comprises one heating or cooling device, at least one temperature sensor, and no more than one metal heat conduction device. The different levels of temperature control units are thermally coupled in a tight applying manner, the last level of temperature control unit is thermally coupled to a heat radiation device, and a thermal insulation housing is surrounded in the side surface of the whole device. Thus, heat can be efficiently and uniformly conducted among the temperature control units to reduce heat exchange between the side surface ofthe whole device and the outside. Each level of temperature control unit controls the temperature independently, and the temperature and duration of the different levels of temperature control units are coordinated and distributed by means of an algorithm, so that the energy utilization rate of the whole device is maximized, and the temperature change rate of the first level of temperature controlunit is improved greatly.

Description

technical field [0001] The invention relates to a temperature control device and method, in particular to a temperature control device and method for nucleic acid reaction. Background technique [0002] In biomolecular research, polymerase chain reaction (PCR) is an important analytical means and method. The PCR method is composed of three processes: double-strand denaturation of the template nucleic acid, primer annealing and template complementary extension, and after multiple cycles, the target nucleic acid in the detected sample is exponentially amplified. [0003] Among them, temperature is an important external factor affecting the process of nucleic acid amplification. Including temperature accuracy and temperature change rate, etc. [0004] The temperature cycle control system is the core technology of PCR equipment. At present, most of the more popular technical solutions in the market are to use unipolar semiconductor refrigeration chips to heat and cool the PCR ...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the problem of slow rate and low efficiency of the temperature control system of the unipolar semiconductor refrigerating sheet when the temperature changes, and proposes a new device and method. The multi-stage heating or cooling devices coordinate with each other to control the temperature to achieve a faster temperature. Change rate and high energy efficiency utilization to adapt to fast and accurate PCR real-time quantitative detection occasions

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