Preparation method of leech active oligopeptide
A leech and activity technology is applied in the field of preparation of leech active oligopeptides, which can solve the problems of small molecular weight of polypeptides, leech odor, and low antithrombin activity of products, and achieve the effects of high bioavailability, high activity and stability.
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[0093] Preparation of leech homogenate and its freeze-dried powder
[0094] Thaw live leeches (Phenibus phenanthae) or frozen products at -80°C, put them in a homogenizer and stir for about 30 minutes until they become mushy and have no lumps, freeze-dry for 24 hours (temperature: -45°C, pressure: 7.9pa ) to obtain freeze-dried powder, sealed and preserved for future use.
Embodiment 1
[0096] The living leech is put into a homogenizer and stirred until it is in the form of a paste and has no lumpy flesh, so as to obtain a homogenate. Freeze-dry the homogenate to obtain a freeze-dried powder, which is sealed and stored for future use. Take 5 g of freeze-dried powder, dissolve in 100 mL of water, add alkaline protease at 1.7% of the weight of the freeze-dried powder, react for 2 hours at a pH of 9 and a temperature of 55°C, and then add pancreatic protease at 2.5% of the weight of the freeze-dried powder Protease was reacted for 3 hours under the conditions of pH 8 and temperature of 60°C, then inactivated at 85°C for 15min, centrifuged, and the supernatant was adjusted to pH 3, adding 5wt% gac and 2.5wt% kaolin (the above supernatant The mass is calculated as 100%), deodorization and decolorization reaction is carried out in a water bath at 60°C for 1 hour, after filtration, the supernatant is taken, the pH is adjusted to neutrality, and freeze-dried to obtai...
Embodiment 2
[0098]The living leech is put into a homogenizer and stirred until it is in the form of a paste and has no lumpy flesh, so as to obtain a homogenate. Freeze-dry the homogenate to obtain a freeze-dried powder, which is sealed and stored for future use. Take 5 g of freeze-dried powder, dissolve in 100 mL of water, add alkaline protease at 1.1% of the weight of the freeze-dried powder, react for 1 hour at a pH of 9 and a temperature of 55 degrees, and then add pancreatic protease at 2.5% of the weight of the freeze-dried powder Protease, pH is 8, temperature is the reaction 4 hours under the condition of 60 ℃, thereafter 85 ℃ inactivation 15min, centrifugation, supernatant adjusts pH to be 3, adds 3wt% gac and 2.5wt% kaolin (the above supernatant The mass is calculated as 100%), deodorization and decolorization reaction is carried out in a water bath at 60°C for 1 hour, after filtration, the supernatant is taken, the pH is adjusted to neutrality, and freeze-dried to obtain leeche...
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