Cell culturing using nanofibers

Abstract

The present invention provides: a cell carrier that contains nanofibers composed of a water-insoluble polysaccharide, preferably chitin or chitosan nanofibers, the carrier is possibly used as a commoncarrier for various operations including i) suspension culturing, ii) differentiation induction, iii) transport and storage under non-freezing conditions, and iv) transplantation of adhesive cells, and v) recovery of a physiologically active substance from a culture supernatant; and continuous execution of, using said carrier, a plurality of operations selected from among i) suspension culturing,ii) differentiation induction, iii) transport and storage under non-freezing conditions, and iv) transplantation of adhesive cells, and v) recovery of a physiologically active substance from a culture supernatant.

Description

technical field [0001] The present invention relates to the use of nanofibers formed of water-insoluble polysaccharides as carriers in the cultivation, storage, transportation or transplantation of cells. Background technique [0002] In recent years, in the field of regenerative medicine, studies have been conducted on culturing pluripotent stem cells such as iPS cells and ES cells, inducing their differentiation into target organ cells, and transplanting the resulting cells. However, there are ethical issues in the establishment of ES cells, and the risk of canceration in iPS cells has become an issue. In addition, with regard to the differentiation of iPS cells into cells constituting target organs, it has been reported that the final degree of differentiation induction is low, and that long-term culture is required to obtain highly differentiated cells, which raises the issue of cost. In view of the above-mentioned problems, adult stem cells such as mesenchymal stem cel...

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Problems solved by technology

Handling of these cells is cumbersome and costly

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