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High-efficiency, rapid and low-cost method of obtaining cassava tissue culture seedlings

A low-cost, tissue culture seedling technology, applied in the field of plant tissue culture, can solve the problems of many medium components and increase the cost of cassava reproduction, and achieve the effects of reducing hormone components, speeding up production and promotion, and the method is simple and easy to implement.

Inactive Publication Date: 2019-11-22
HAINAN UNIVERSITY
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  • Summary
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

More hormones are used in the medium, and there are many components in the medium, which increases the cost of cassava propagation

Method used

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  • High-efficiency, rapid and low-cost method of obtaining cassava tissue culture seedlings
  • High-efficiency, rapid and low-cost method of obtaining cassava tissue culture seedlings

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1: the preparation of cassava tissue culture seedling

[0015] 1. Preparation of semi-solid medium:

[0016] The semi-solid medium was prepared according to the formula in Table 1, with a pH value of 5.8, sterilized under high temperature and high pressure at 115°C for 30 minutes, and solidified for later use.

[0017] Table 1 Medium composition and dosage

[0018]

[0019] 2. Surface disinfection of cassava explants:

[0020] The surface disinfection of cassava explants was mainly carried out using 75% alcohol and sodium hypochlorite solution. Take the young twigs with bud eyes of Huanan No. 124 cassava, cut off the leaves, and keep the petiole about 3cm long, wash it with detergent water and then rinse it with running water for 20 minutes, put it in an ultra-clean workbench, and rinse it with 75% alcohol for 10 seconds , washed twice with sterile water, and then soaked in 1% sodium hypochlorite solution by volume for 10 minutes, shaking from time to t...

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Abstract

The invention discloses a high-efficiency, rapid and low-cost method of obtaining cassava tissue culture seedlings. The method comprises the steps of A, preparation of a semisolid culture medium; B, surface disinfection of cassava explants; C, starting culture and survival of the tissue culture seedlings. The method has the advantages that components of a traditional culture medium are reduced onthe basis of ensuring that the cassava tissue culture seedlings can grow normally, the cassava tissue culture seedlings can also be obtained in a short time, the growth vigor of the tissue culture seedlings is more normal, and the cost for asexual reproduction of cassava is reduced.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for asexual propagation of cassava, in particular to a method for obtaining cassava tissue culture seedlings efficiently, quickly and at low cost through asexual reproduction. Background technique [0002] Cassava (Manihot esculenta Crantz), also known as cassava and cassava, is a plant of the genus Cassava in the Euphorbiaceae family. It is a drought-resistant and barren-resistant crop and a shrub-like perennial crop. Stems erect, woody, 2-5m high, single leaves alternate palmately parted, papery, lanceolate. Unisexual flowers, panicles, terminal, hermaphroditic. Cassava was introduced to China in the 1820s. It was first cultivated in Gaozhou, Guangdong Province, and then introduced to Hainan Island. It is now widely distributed in South China, with Guangxi, Guangdong and Hainan being the most cultivated. There are also introduction trials in ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 施海涛何梅
Owner HAINAN UNIVERSITY