Application of bacteroides fragilis or Akkermansia muciniphila to preparation of drug for preventing or treating tumors

A technology of Bacteroides fragilis and myxobacteria, applied in the field of biomedicine

Active Publication Date: 2019-11-26
REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, there are currently no reports on the use of intestinal bacteria including Bacteroides fragilis and / or Akkermansia to promote the infiltration and / or accumulation of CD8+ T cells in the tumor microenvironment for tumor prevention and / or treatment

Method used

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  • Application of bacteroides fragilis or Akkermansia muciniphila to preparation of drug for preventing or treating tumors
  • Application of bacteroides fragilis or Akkermansia muciniphila to preparation of drug for preventing or treating tumors
  • Application of bacteroides fragilis or Akkermansia muciniphila to preparation of drug for preventing or treating tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1 Bacteroides fragilis culture

[0043] training method

[0044] Step 1: Take a freeze-dried and preserved strain of Bacteroides fragilis (purchased from ATCC official website), add 200 μL TSB medium, reconstitute, draw 20 μL, streak the blood plate, and pump air from the gas control system of the anaerobic tank Then in a biochemical incubator at 37°C, anaerobic culture for 48h;

[0045] Step 2: pick a single clone colony into 10mL TSB medium, 37°C, anaerobic culture for 12h;

[0046] Step 3: Take a bottle of 500mL TSB medium, insert 1% (v / v) strains, and culture anaerobically at 37°C for 48 hours;

[0047] Step 4: Take the bacterial solution and centrifuge at 6000rpm for 10min. Wash twice with normal saline, and finally reconstitute the sludge with normal saline for use and count the viable bacteria.

Embodiment 2

[0048] Embodiment 2 Ackerman's myxobacteria culture

[0049] training method

[0050] Step 1: Take a freeze-dried strain of Akkermansia muciniphila (purchased from ATCC official website), add 200 μL TSB medium, reconstitute, draw 20 μL, streak the blood plate, anaerobic tank gas control system After pumping, incubate in a biochemical incubator at 37°C for 48 hours under anaerobic conditions;

[0051] Step 2: pick a single clone colony into 10mL TSB medium, 37°C, anaerobic culture for 12h;

[0052] Step 3: Take a bottle of 500mL TSB medium, insert 1% (v / v) strains, and culture anaerobically at 37°C for 48 hours;

[0053] Step 4: Take the bacterial solution and centrifuge at 6000rpm for 10min. Wash twice with normal saline, and finally reconstitute the sludge with normal saline for use and count the viable bacteria.

Embodiment 3

[0054] Example 3 Bacteroides fragilis promotes the infiltration and / or accumulation of CD8+ T cells in the tumor microenvironment and the effect of treating tumors

[0055] figure 1 Schematic diagram of the experimental flow for detecting the accumulation of CD8+ T cells in the tumor microenvironment and the therapeutic effect of B. fragilis and inactivated B. fragilis.

[0056] 1. Training method

[0057] The culture method of Bacteroides fragilis is the same as in Example 1.

[0058] 2. Sample preparation

[0059] 1) Preparation of live Bacteroides fragilis ZY-312 cells

[0060] Step 1: Take a freeze-dried strain (purchased through commercial channels), add 200ul freeze-dried culture medium, redissolve, absorb 20ul, draw a line on the blood plate, and perform biochemical culture after the gas control system of the anaerobic tank is pumped In the box at 37°C, anaerobic culture for 48h;

[0061] Step 2: Pick a monoclonal colony and insert it into 10mL TSB medium, and cult...

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Abstract

The invention relates to application of bacteroides fragilis or Akkermansia muciniphila to preparation of a drug for preventing or treating tumors. The drug promotes infiltration and / or accumulation of CD8 positive cytotoxic T lymphocytes in a tumor microenvironment. The invention further provides application of a drug composition, food, a health care product or a food additive for preventing or treating tumors, and the drug composition, the food, the health care product or the food additive includes the bacteroides fragilis or the akkermansia muciniphila and promotes infiltration and / or accumulation of the CD8 positive cytotoxic T lymphocytes in the tumor microenvironment.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of Bacteroides fragilis or Akkermansia in the preparation of drugs for preventing and / or treating tumors. Background technique [0002] Cancer has become the "first killer" of human beings. The "Global Cancer Report 2014" published by the World Health Organization (WHO) predicts that global cancer cases will show a rapid growth trend, from 14 million in 2012 to 19 million in 2025, and will reach 24 million in 2035 . There are about 7 million new cancer patients every year in the world, and about 5 million cancer patients die. On average, one person dies of cancer every 6 seconds. Chemotherapy is currently recognized as the main treatment, and its main purpose is to kill cancer cells in the body. But while chemotherapy drugs kill cancer cells, they also damage normal cells in the body. Breast cancer is currently the most common type of malignant tumor, and it is also...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P35/00A23L33/135
CPCA61K35/741A61P35/00A23L33/135A23V2002/00A61K2300/00A23V2200/308A23V2250/206C12N1/20A61K35/74
Inventor 曾谷城
Owner REVAISSANT SHENZHEN BIOSCIENCES CO LTD
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