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Targeted perfluorocarbon oxygen-carrying microspheres for magnetic thermotherapy and preparation method and application thereof

A technology of perfluorocarbon and magnetic hyperthermia, which is applied in the field of nanomaterials and biomedical materials, can solve problems such as toxicity and patient life-threatening, and achieve the effects of improving efficacy, good biodegradability, and good biocompatibility

Inactive Publication Date: 2019-11-26
TONGJI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many substances, such as hemoglobin, manganese dioxide, etc., have been used to alleviate the hypoxic microenvironment of tumors, but they all have certain limitations
For example, hemoglobin can cause acute nephrotoxicity and acute hypertension, which pose a serious threat to the patient's life, while the level of tumor reoxygenation achieved by manganese dioxide is affected by the H of the tumor environment. 2 o 2 amount limit, and Mn 2+ inherently toxic

Method used

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Examples

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preparation example Construction

[0046] The preparation method of the targeted perfluorocarbon oxygen-carrying microspheres for magnetic hyperthermia of the present invention comprises the following steps:

[0047](1), preparation of PFCs aseptic emulsion: add egg yolk lecithin into Tyrode solution (Tyrode solution), use ultrasonic emulsification machine to emulsify 4-6 times at 25-30 ℃, each duration is 5- 10s, with an interval of 30-60s, to obtain the basic emulsion; add a perfluorocarbon stock solution with a volume fraction ≥ 97% in the basic emulsion, and use a ultrasonic emulsification machine to emulsify 4-7 times, and the emulsification time is 5-10s each time. After an interval of 30-60s, the original perfluorocarbon emulsion was obtained and set aside; the original perfluorocarbon emulsion was placed in a hyperbaric oxygen chamber to fully absorb oxygen to obtain a perfluorocarbon emulsion; The fluorocarbon emulsion is sterilized by filtration to obtain a perfluorocarbon aseptic emulsion for subsequ...

Embodiment 1

[0070] The preparation method of the targeted perfluorotributylamine oxygen-carrying microspheres for magnetic hyperthermia in this embodiment comprises the following steps:

[0071] (1), egg yolk lecithin was added to the Tyrode solution, and the mass percentage of egg yolk lecithin was 17%. At 25°C, it was emulsified 6 times using a ultrasonic emulsifier, with a power of 450W, and the duration of each time was 10s. 30s, get the basic emulsion;

[0072] (2), add the perfluorotributylamine stock solution with a volume fraction of 97% in the basic emulsion, the volume ratio of the basic emulsion and the perfluorotributylamine stock solution is 1:1, use a ultrasonic emulsifier to emulsify 7 times according to the above steps, The power is 450W, the time of each emulsification is 10s, and the interval between each emulsification is 60s, and the original emulsion of perfluorotributylamine is obtained, which is ready for use;

[0073] (3), put the original emulsion of perfluorotri...

Embodiment 2

[0083] The preparation method of targeted perfluorocyproamine and perfluorooctyl bromide oxygen-carrying microspheres for magnetic hyperthermia in this embodiment comprises the following steps:

[0084] (1) Add egg yolk lecithin to the Tyrode solution, the mass percentage of egg yolk lecithin is 20%, and emulsify 4 times at 25°C using a ultrasonic emulsifier, with a power of 350W, and the duration of each time is 5s. 30s, get the basic emulsion;

[0085] (2), adding volume fraction in base emulsion is 97% perfluorocyclopropylamine and perfluorooctyl bromide stock solution, the volume ratio of base emulsion, perfluorocyclopropylamine and perfluorooctyl bromide stock solution is 1.4:1, Use a ultrasonic emulsifier to emulsify 4 times according to the above steps, the power is 350W, the emulsification time is 5s each time, and the interval is 30s, to obtain the original emulsion of perfluorocyclopropylamine and perfluorooctyl bromide, and set aside;

[0086] (3), the original emu...

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Abstract

The invention provides a targeted perfluorocarbon oxygen-carrying microsphere for magnetic hyperthermia and a preparation method and application thereof. The preparation method comprises emulsifying perfluorocarbon into an emulsion, the perfluorocarbon emulsion is soluble in water and carrying oxygen, oxygenating and disinfecting the perfluorocarbon emulsion, wrapping the emulsion and magnetic nanoparticles in chitosan to form microspheres, and linking surface of the microspheres with polyethylene glycol and folic acid to improve water solubility of the microspheres and achieve a targeting function. In the preparation method, perfluorocarbon is used as an oxygen-carrying material and wrapped in chitosan together with the magnetic nanoparticles, the surface of the obtained microspheres is modified with polyethylene glycol molecules to improve the water solubility and grafted with folic acid molecules to form microspheres with a core-shell structure. The microspheres can achieve a sustained release effect of oxygen, the shell material has good biocompatibility and targeting, and the magnetic nanoparticles can exert magnetic thermotherapy in a magnetic field, thereby the ability of the microspheres to kill tumors is improved. The microspheres can alleviate hypoxia in tumor site and improve an effect of external adjuvant therapy.

Description

technical field [0001] The invention belongs to the technical field of nanomaterials and biomedical materials, and in particular relates to a targeted perfluorocarbon oxygen-carrying microsphere for magnetic hyperthermia and its preparation method and application. Background technique [0002] Hypoxia caused by abnormal tumor blood vessels and excessive cell proliferation is a common phenomenon in almost all solid tumors. Studies have shown that hypoxia is one of the important reasons for tumor treatment failure. Especially for radiotherapy, oxygen is an essential element for the generation of tumor-killing reactive oxygen species. Thus, the hypoxic tumor microenvironment is often an important factor in radiotherapy resistance. Many substances, such as hemoglobin and manganese dioxide, have been used to alleviate the hypoxic microenvironment of tumors, but they all have certain limitations. For example, hemoglobin can cause acute nephrotoxicity and acute hypertension, whic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K47/60A61K47/54A61K41/00A61K31/02A61P35/00B82Y5/00B82Y25/00A61K33/00
CPCA61K31/02A61K33/00A61K41/0052A61K47/545A61K47/60A61K47/6939A61P35/00B82Y5/00B82Y25/00A61K2300/00
Inventor 袁伟忠周子璇
Owner TONGJI UNIV
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