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A method for promoting stem elongation in in vitro culture of cherry blossoms

A technology of in vitro culture and cherry blossoms, which is applied in the field of plant tissue culture, can solve the problem of difficult elongation and growth of stem segments, and achieve the effect of overcoming short growth of plants and promoting stem elongation

Active Publication Date: 2021-11-30
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are mainly embryo culture, bud differentiation culture, leaves and other organ culture, but the cherry blossom tissue culture seedlings are relatively short and the stems of cherry blossoms are difficult to elongate and grow.

Method used

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  • A method for promoting stem elongation in in vitro culture of cherry blossoms
  • A method for promoting stem elongation in in vitro culture of cherry blossoms

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Experimental program
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Effect test

Embodiment 1

[0021] A method for promoting stem elongation in cherry blossom in vitro culture, the specific steps are as follows:

[0022] (1) Choose sunny weather in April, around ten o'clock in the morning, select annual branches with strong mother plants, no pests and diseases, full axillary buds, and good growth conditions, cut off the leaves, and cut them into 2-3cm stems with 1-2 axillary buds segments, as explants;

[0023] (2) Put the explants in a sterilized beaker, seal the mouth with gauze, rinse under tap water for 1.5-2h; soak with 5% sodium hypochlorite for 15min, rinse with sterile water for 2-3 times; 0.2% benzene Soak in special + 0.2% captan for 10 minutes, rinse with sterile water, and set aside;

[0024] (3) Inoculate the sterilized explants in WPM+0.2mg·L -1 6-BA+0.1mg·L -1 On NAA medium (pH 5.8-6.0), cultured under cold treatment at 5.5°C for 42 days; irradiated with red light, the light intensity was 20 μmol / (m 2 s), the light time is 12h / d;

[0025] (4) After t...

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Abstract

The invention discloses a method for promoting stem elongation in cherry blossom in vitro culture, and belongs to the technical field of plant tissue culture. A method for promoting stem elongation in cherry blossom in vitro culture disclosed by the present invention, through sterilizing explants, cold treatment and culturing under red light; overcomes the short growth of cherry blossom seedlings and the obstruction of stem elongation in the process of tissue culture problems; use low temperature to break the dormancy of buds, use hormones and red light to promote stem growth, and the length of stem segments reaches 4.23cm.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for promoting stem elongation in cherry blossom in vitro culture. Background technique [0002] Cherry blossoms (Cerasus sp.) belong to Rosaceae, Lisubaceae, and Sakura, and the height of the tree is 15-25m. Bark chestnut brown, smooth; leaves ovate to ovate-elliptic, leaf ends caudate, margins with sharp single or heavy serrations, tooth ends short awn-like, glabrous on both sides. Flowers white or light pink, single petals, 3-5 flowers in a short corymb raceme, no fragrance, calyx tube bell-shaped and glabrous. Drupe globose, first red and then purple-brown. The flowering period is April, and the flowers and leaves are placed together. Cherry blossoms like sunshine and deep, fertile and well-drained soil. They have a strong adsorption capacity for harmful gases such as sodium hydroxide and carbon dioxide, so they can be used to purify the air. The main...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 刘国华王永平王金玲
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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