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Embryo culture and seedling raising method for taxus wallichiana

A technology of embryo culture and yew, applied in the field of plant propagation, can solve the problems of many manpower, material resources, low humidity, high humidity, etc., and achieve the effect of improving the seedling rate, fast method, and improving the germination rate

Inactive Publication Date: 2019-12-03
JIANGSU HONGDOUSHAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the natural environment is difficult to control artificially. For example, if there is no rain for a long time, the humidity will be low. In the case of rainy weather and long-term rainfall, the humidity will be high.
However, the cutting propagation of yew has relatively high requirements on light, temperature, and humidity, and the natural environment cannot meet its propagation requirements, requiring more manpower and material resources, and the survival rate is lower than that of seed propagation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for cultivating seedlings of yew embryos, comprising the following steps:

[0016] (1) Remove the arils from mature yew seeds, rinse them with linear tap water for 5 minutes, soak them in distilled water for 1 hour, then disinfect them with 70% alcohol for 1 minute, sterilize them with 0.1% mercury liter for 20 minutes, rinse them with sterile water, and put them in Embryos were stripped on sterile filter paper as explants;

[0017] (2) Inoculate the explants obtained in step (1) into MS medium, and culture them in dark at 24°C for 150 days until the explants germinate; add 1g of hydrolyzed casein and 1g of yeast extract to each liter of MS medium , 30g sucrose and 7g agar;

[0018] (3) Move the germinated explants obtained in step (2) to the light condition and cultivate them for 20 days until the bud length is 2cm, the culture temperature is 24-℃, the light is 10h per day, and the light intensity is 2000lx;

[0019] (4) Transfer the seedlings obtained in st...

Embodiment 2

[0023] A method for cultivating seedlings of yew embryos, comprising the following steps:

[0024] (1) Remove the arils from mature yew seeds, rinse them with linear tap water for 10 minutes, soak them in distilled water for 2 hours, then disinfect them with 70% alcohol for 2 minutes, sterilize them with 0.1%   liter mercury for 30 minutes, rinse them with sterile water, and put them in Embryos were stripped on sterile filter paper as explants;

[0025] (2) Inoculate the explants obtained in step (1) into MS medium, and culture them in dark at 26°C for 20 days until the explants germinate; add 0.8g hydrolyzed casein, 0.8g Yeast extract, 28g sucrose and 6g agar;

[0026] (3) Transfer the germinated explants obtained in step (2) to culture under light conditions for 25 days until the bud length is 2 cm, the culture temperature is 26°C, the light is 12 hours a day, and the light intensity is 2000lx;

[0027] (4) Transfer the seedlings obtained in step (3) to a test tube for sep...

Embodiment 3

[0031] A method for cultivating seedlings of yew embryos, comprising the following steps:

[0032] (1) Remove the arils from the mature Yunnan yew seeds, wash them with linear tap water for 70 minutes, then soak them in distilled water for 1.5 hours, then disinfect them with 70% alcohol for 1.5 minutes, 0.1% mercury liter for 25 minutes, and rinse them with sterile water. Embryos were exfoliated on sterile filter paper as explants;

[0033] (2) Inoculate the explants obtained in step (1) into MS medium, and culture them in dark at 25°C for 17 days until the explants germinate; add 1.2g hydrolyzed casein, 1.2g Yeast extract, 32g sucrose and 8g agar;

[0034] (3) Move the germinated explants obtained in step (2) to light for 23 days until the bud length is 2 cm, the culture temperature is 25°C, the light is 11 hours a day, and the light intensity is 2000lx;

[0035] (4) Transfer the seedlings obtained in step (3) to a test tube and culture them alone for 23 days, add 1 / 4 WPM m...

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Abstract

The invention relates to a plant propagation method, in particular to an embryo culture and seedling raising method for taxus wallichiana. The method includes the following steps of explant treatment,MS culture medium culture, test tube culture, and seedling hardening and transplanting. According to the embryo culture and seedling raising method for taxus wallichiana, embryos are separated from seeds and subjected to in-vitro culture, and the influence of the natural environment on germination of the embryos is avoided; different mediums are adopted at the culture stage, different substancesare added to the different mediums, and the germination rate and the seedling rate are increased; a fast and convenient method is provided for propagation of taxus wallichiana.

Description

technical field [0001] The invention relates to a plant propagation method, in particular to a yew embryo culture and seedling raising method. Background technique [0002] Taxus is a shallow-rooted plant with inconspicuous main roots and well-developed lateral roots. It is recognized as an endangered natural and rare anti-cancer plant in the world. Due to the slow growth rate and poor regeneration ability of yew under natural conditions, it has been used worldwide for a long time. There is no large-scale yew raw material forest base in China. [0003] There are two commonly used propagation methods of yew, seed propagation and cutting propagation. Among them, seed propagation needs to go through a germination process, and outdoor natural wet sand storage is often used to treat the seeds with stratification to increase the germination rate of the seeds. However, the natural environment is difficult to control artificially. For example, if there is no rain for a long time, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G24/15A01G31/00
CPCA01G31/00A01H4/001A01H4/008A01G24/15
Inventor 朱波风
Owner JIANGSU HONGDOUSHAN BIOLOGICAL TECH
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