Degenerate primer set for one-time human immunodeficiency virus (HIV) genotypic resistance detection and application of degenerate primer set

Abstract

The invention provides a degenerate primer set for one-time human immunodeficiency virus (HIV) genotypic resistance detection and application of the degenerate primer set, and relates to the technicalfield of virus detection. The degenerate primer set includes a reverse transcription PCR primer, a nest PCR amplification primer and a sequencing universal primer; the reverse transcription PCR primer includes WC-pol-F and WC-pol-R; the nest PCR amplification primer includes WC-nest-F and WC-nest-R; and the sequencing universal primer includes HBX2-3017R, HBX2-3785R and HBX2-4561R. The degenerateprimer set is applicable to genotypic resistance detection of most of HIV-1 strains prevalent in China, a gene coding region of HIV protease-everse transcriptase-integrase can be amplified at a time,compared with a conventional method, the cost is lower, the detection speed is higher, and meanwhile the success rate of detection is higher.

Description

technical field [0001] The invention relates to the technical field of virus genotype detection, in particular to a degenerate primer set for one-time detection of HIV genotype drug resistance and its application. Background technique [0002] Human Immunodeficiency Virus (HIV) attacks the human immune system and seriously threatens human life and health. The emergence of drug-resistant mutant strains of HIV not only reduces the effect of antiviral treatment and increases the number of deaths; more importantly, the prevalence of drug-resistant strains will bring serious public health problems. For this reason, detection of HIV genotypic drug resistance mutations is very necessary. The total length of the HIV-1 genome is about 9kb. It is a single-stranded antisense RNA virus. The pol region encodes protease, reverse transcriptase and integrase, ranging from 2357nt to 5095nt, and is the main region that causes drug resistance mutations. At present, several companies have mat...

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defects of high failure rate, high detection cost and long detection time of existing HIV genotype drug resistance detection, the present invention provides a new degenerate primer set and kit for HIV genotype drug resistance detection and The detection method uses the degenerate primer set provided by the present invention to amplify the HIV protease-reverse transcription PCR enzyme-integrase coding gene at one time, which reduces the clinical detection cost, saves time, and increases the detection success rate

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