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Application of a kind of eucalyptus eucalyptus essential oil and its compound bacterial agent in the preparation of insect repellent for seawater fish

A technology of compound bacterial agent and eucalyptus essential oil, applied in application, fish farming, climate change adaptation and other directions, can solve problems such as damage to aquatic animal health, hidden dangers to consumer safety, unstable drug dosage, etc., to reduce bacterial infection and save energy. Breeding costs, the effect of a wide range of use

Active Publication Date: 2021-11-02
SHANDONG LONGCHANG ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to simply pursue immediate results, traditional medicines will cause excessive heavy metals and unstable drug dosages, which will destroy the stability of water quality, damage the health of aquatic animals and reduce their immunity. In severe cases, it will directly lead to death. , ultimately reducing production efficiency and bringing safety hazards to consumers

Method used

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  • Application of a kind of eucalyptus eucalyptus essential oil and its compound bacterial agent in the preparation of insect repellent for seawater fish
  • Application of a kind of eucalyptus eucalyptus essential oil and its compound bacterial agent in the preparation of insect repellent for seawater fish
  • Application of a kind of eucalyptus eucalyptus essential oil and its compound bacterial agent in the preparation of insect repellent for seawater fish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] One, the preparation of Eucalyptus eucalyptus essential oil

[0033] The preparation process of Eucalyptus eucalyptus essential oil in the present invention is as figure 1 As shown, first put the leaves of Eucalyptus eucalyptus in Australia into the washing tower for cleaning, put them in the dryer for drying, and then pulverize them in the pulverizer and then enter the steam distillation kettle, condenser and oil-water stratifier for separation The oil phase and the water phase, the water phase enters the ether extractor to separate the waste water, the liquid containing ether enters the ether recovery tower to recover ether, and the recovered ether and oil phase liquid are added to the solid bed adsorption dryer to further remove the ether in the oil phase Finally, the oil phase liquid is added to a vacuum rectification tower for rectification to remove impurity oils to obtain eucalyptus multibract essential oil.

[0034] The essential oil of Eucalyptus multibract pr...

Embodiment 2

[0044] The eucalyptus eucalyptus essential oil prepared in Example 1 was used for the insect repellent experiment. The test site was selected in Penang, Malaysia, and sea bass were cultured in seawater cages with a size of 0.1 kg. After parasites were detected in the gills of the perch, 1 kg of eucalyptus eucalyptus essential oil was used to mix 1 ton of feed, and the feeding was carried out according to normal feeding and management, and fed continuously for 7 days, 3 times a day. After 7 days, check the gill parasites of the golden perch again. Such as Figure 4 As shown, at the beginning of feeding, the fins of the golden perch were infected with sea lice; after 7 days of feeding, the sea lice on the gills of the golden perch were detected to fall off, which shows that the essential oil of Eucalyptus multibud has an obvious deworming effect on the golden perch.

Embodiment 3

[0046] Golden pomfret was cultured in Zhanjiang cages, and the gills of the fish were found to be red and swollen, with a lot of mucus. Subsequent microscopic examination found that there were many finger ringworms in the gills of the fish. Using 1 kg of eucalyptus eucalyptus essential oil compound microbial agent prepared in Example 1 to mix with 1 ton of feed, carry out according to normal feeding and management, and feed continuously for 7 days, 3 times a day. After 7 days, check the gill parasites of the golden pomfret again. As shown in Figure 5, at the initial stage of feeding, the gills of the golden pomfret were infected with ring worms; after 7 days of feeding, the ring worms in the gills disappeared, which shows that the compound bacterial agent of Eucalyptus eucalyptus essential oil has obvious effect on golden pomfret. Insect repellent effect.

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Abstract

The present invention provides an application of Eucalyptus multibract essential oil and its composite bacterial agent in the preparation of an insect repellant for seawater fish. Not less than 5×10 8 CFU / g of Streptomyces griseus HL‑50, wherein the preservation number of Streptomyces griseus HL‑50 is CGMCC No.18424, and the main active ingredient of Eucalyptus multibractosa eucalyptus essential oil is 1,8‑cineole. The eucalyptus eucalyptus essential oil compound microbial agent of the present invention is simple to prepare and use, and it only needs to be uniformly mixed in the edible feed of seawater fish according to the dosage of 0.5-1.5 kg / ton of feed and continuously fed for 4-7 days to achieve It can effectively drive away various parasites infected by seawater fish and has antibacterial effect, and the compound bacterial agent is non-toxic and harmless, is suitable for long-term use of fish, reduces breeding costs, and has broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to the application of eucalyptus eucalyptus essential oil and its compound bacterial agent in the preparation of an insect repellant for seawater fish. Background technique [0002] Essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots or fruits of plants through steam distillation, extrusion, cold soaking or solvent extraction, most of which have a lighter specific gravity than water. The relative density is generally in the range of 0.8 to 1.17. Essential oils are sensitive to heat and light, so they need to be stored in dark containers and in a cool place, and essential oils are now mostly used in skin care products and aromatherapy because of their special aroma and the characteristics of being soluble in organic solvents, but the function of plant essential oils Various, in addition to the traditional fragrance, soothi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A23K50/80A23K10/30A23K10/18
CPCA23K10/18A23K10/30A23K50/80A01K61/10Y02A40/81
Inventor 曹爱智籍立民娄倩倩
Owner SHANDONG LONGCHANG ANIMAL HEALTH PROD
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