Method for producing ethanol from grain
A technology for grains and raw materials, applied in the field of bioethanol production, can solve the problems of long fermentation time, high energy consumption, starch gelatinization, etc., and achieve the effects of reducing steam and water consumption and increasing utilization rate
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Embodiment 1
[0044] The grain raw materials are removed from impurities, and after the removal of impurities, the raw materials are crushed with a pulverizer to a size below 20 mesh. After crushing, process water or clear liquid reuse water is used for slurry adjustment, and the concentration of starch slurry is controlled at 30%. Transport the prepared starch slurry to the liquefaction tank, add cellulase, mannanase, pectinase, acid-resistant liquefaction enzyme, and carry out the first liquefaction. The dosage of the above-mentioned enzymes is respectively: the dosage of cellulase is 2.5u / g raw material, 7u / g raw material for mannanase, 10u / g raw material for pectinase, 6u / g raw material for acid-resistant liquefaction enzyme, the temperature is 55°C and the time is 40min in the first stage of liquefaction. After the first stage of liquefaction, the second stage of liquefaction was carried out at 85°C for 60 minutes. During the second stage of liquefaction, only acid-resistant liquefacti...
Embodiment 2
[0046] The preparation process is the same as in Example 1, except that in the first stage of the liquefaction process, the dosage of cellulase is 3.0u / g raw material, the dosage of mannanase is 10u / g raw material, and the dosage of pectinase is 10u / g raw material , acid-resistant liquefaction enzyme is 8.0u / g raw material. The ethanol concentration can reach 142g / L after the fermentation time is 50h.
Embodiment 3
[0048] The preparation process is the same as that in Example 1, except that in the second stage of the liquefaction process, the dosage of the acid-resistant liquefaction enzyme is 12u / g raw material. The ethanol concentration can reach 135g / L after the fermentation time is 50h.
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