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A seed breeding method for improving salt tolerance of head cabbage

A technology of head cabbage and cultivation method, which is applied in horticultural methods, botany equipment and methods, plant regeneration, etc., can solve the problems that affect the content of vitamin C in head cabbage and is not suitable for planting, so as to improve the germination rate and prevent The effect of loss and enhancement of permeability

Active Publication Date: 2021-03-09
铜仁万山九丰现代农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Heading cabbage likes mild climate, can tolerate low temperature and relatively high temperature. The suitable temperature for heading period is 18-20°C, and the adaptable temperature range is 7-25°C. But heading cabbage is not suitable for planting in saline-alkali soil, which will affect Vitamin C Content in Heading Cabbage

Method used

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  • A seed breeding method for improving salt tolerance of head cabbage
  • A seed breeding method for improving salt tolerance of head cabbage
  • A seed breeding method for improving salt tolerance of head cabbage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A seed cultivation method for improving the salt-tolerant performance of head cabbage, comprising the steps of:

[0023] S1 steam treatment: After smearing the seeds with propolis with a thickness of 3mm, place them in a steam environment at 130°C for 15s;

[0024] S2 dark culture: put the seeds into tissue culture medium A for dark culture;

[0025] S3 shaking treatment: place the seeds in the nutrient solution and shake vertically for 20 minutes, wherein the shaking frequency is 300 times / min;

[0026] S4 light culture: put the seeds into tissue culture medium B for light culture.

[0027] The tissue culture medium A: 1 / 2MS+0.8mg / L6-BA+1.2mg / LNAA+25g / L sucrose+5g / L agar.

[0028] The working conditions of the dark cultivation are as follows: the cultivation time is 8 hours, and the cultivation temperature is 25°C.

[0029] The nutrient solution includes the following raw materials in parts by weight: 5 parts of monoammonium phosphate, 4 parts of calcium nitrate, 9 ...

Embodiment 2

[0033] A seed cultivation method for improving the salt-tolerant performance of head cabbage, comprising the steps of:

[0034] S1 steam treatment: After smearing the seeds with propolis with a thickness of 1mm, place them in a steam environment at 120°C for 10s;

[0035] S2 dark culture: put the seeds into tissue culture medium A for dark culture;

[0036] S3 shaking treatment: place the seeds in the nutrient solution and shake vertically for 10 minutes, wherein the shaking frequency is 100 times / min;

[0037] S4 light culture: put the seeds into tissue culture medium B for light culture.

[0038] The tissue culture medium A: 1 / 2MS+0.8mg / L6-BA+1.2mg / LNAA+25g / L sucrose+5g / L agar.

[0039] The working conditions of the dark culture are: the culture time is 5 hours, and the culture temperature is 15°C.

[0040] The nutrient solution includes the following raw materials in parts by weight: 3 parts of monoammonium phosphate, 2 parts of calcium nitrate, 7 parts of urea, 1 part o...

Embodiment 3

[0044] A seed cultivation method for improving the salt-tolerant performance of head cabbage, comprising the steps of:

[0045] S1 steam treatment: After smearing the seeds with propolis with a thickness of 2mm, place them in a steam environment at 125°C for 12s;

[0046] S2 dark culture: put the seeds into tissue culture medium A for dark culture;

[0047] S3 shaking treatment: place the seeds in the nutrient solution and shake vertically for 15 minutes, wherein the shaking frequency is 200 times / min;

[0048] S4 light culture: put the seeds into tissue culture medium B for light culture.

[0049] The tissue culture medium A: 1 / 2MS+0.8mg / L6-BA+1.2mg / LNAA+25g / L sucrose+5g / L agar.

[0050] The working conditions of the dark cultivation are as follows: the cultivation time is 6.5 hours, and the cultivation temperature is 20°C.

[0051] The nutrient solution includes the following raw materials in parts by weight: 4 parts of monoammonium phosphate, 3 parts of calcium nitrate, ...

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Abstract

The invention relates to the technical field of head cabbage seed cultivation, in particular to a seed cultivation method for improving the salt tolerance of head cabbage. The method comprises the following steps of S1, steam treatment, wherein after being coated with propolis with the thickness of 1-3 mm, seeds are placed in a steam environment at 120-130 DEG C for treatment for 10-15 s; S2, darkculture, wherein the seeds are put into a tissue culture medium A for dark culture; S3, oscillation treatment, wherein the seeds are placed in a nutrient solution for vertical oscillation for 10-20 minutes, and the oscillation frequency is 100-300 times per minute; S4, illumination culture, wherein the seeds are put into a tissue culture medium B for illumination culture. By utilizing the method,the dormancy of the seeds can be rapidly and effectively released, the dormancy period is shortened to be about 30 days, germination of the seeds is accelerated, and the germination rate of the seedsis increased.

Description

technical field [0001] The invention relates to the technical field of cabbage seed cultivation, in particular to a seed cultivation method for improving the salt tolerance of cabbage. Background technique [0002] my country is a country with large saline-alkali land in the world, and the area of ​​saline-alkali soil accounts for about 1.03% of the country's land area. Therefore, the improvement of saline-alkali land has caused widespread planting in the country and even the world. Among many improvement measures, planting plants has the highest economic benefits. Therefore, the applicant tried to improve saline-alkali land by using head cabbage. [0003] Heading cabbage is a plant of the genus Brassica and Brassica. It is a variety of cabbage, also known as cabbage, cabbage, pimple white, cabbage, cabbage, cabbage, lotus white, small iron head, etc. Strong, easy to store, resistant to transportation, high yield, dark green to green leaves, smooth leaf surface, thick mesop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 陈守刚罗勇冯玉萍
Owner 铜仁万山九丰现代农业科技有限公司
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