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Method for rapid propagation of seedlings through tillandsia lateral bud tissue culture

A technology of Tillandsia truncatula and tissue culture rapid propagation, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of large number of leaves and no promotion effect on seedling growth, and achieve fast growth of seedlings and harvest materials Convenience and easy operation

Inactive Publication Date: 2019-12-10
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Li Ning compared the growth of the seeds of the Strykert variety of air pineapple on the MS (or only agar) medium with three different nutritional components and the addition of a certain type of hormone (auuxin or cytokinin) to the MS medium. , the results show that although the seeds can grow seedlings on the three media, the MS medium makes the seedlings grow the fastest; in addition, the seedlings added with auxins (NAA and IBA) in the MS medium obviously grow faster than those without Faster, more leaves; Adding cytokinin (6-BA) growth regulators in MS medium has no effect on seedling growth

Method used

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  • Method for rapid propagation of seedlings through tillandsia lateral bud tissue culture
  • Method for rapid propagation of seedlings through tillandsia lateral bud tissue culture
  • Method for rapid propagation of seedlings through tillandsia lateral bud tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Selection and disinfection of lateral buds of air pineapple

[0029] 1. Selection of lateral buds: Considering the particularity of the growth of lateral buds of air pineapple, such as slow growth, relatively weak growth ability, relatively fragile leaves and stems, and some silver leaf varieties often have a small amount of water at the top of the buds, etc. Increased the difficulty of collecting and disinfecting the lateral bud explants; so many details need to be noticed in advance during the collection and disinfection of the lateral bud explants of terrier pineapple. Usually, the more appropriate time to collect materials is when the temperature has not risen in early spring or when the weather turns cool in late autumn and early winter. If the collection is under high temperature and high humidity conditions in midsummer, the weather needs to be sunny for at least 3 consecutive days. Diseased explants. In order to ensure a high survival rate, it is bet...

Embodiment 2

[0034] Embodiment 2: the induction and differentiation of clustered buds and the rapid propagation and proliferation of sprouts

[0035] 1. Induced differentiation culture of clustered buds: Transfer the above-mentioned aseptic lateral bud explants (lateral bud embryogenic callus) to the following clustered bud induction differentiation medium to induce clustered shoots, and cultivate them under 1500-2000LX light for 56 days , to observe the growth and development of clustered buds (see Image 6 , Figure 7 , Figure 8 and Table 2).

[0036] Table 2 Effects of different clustering bud induction medium formulations on the growth and development of clustering buds

[0037]

[0038]

[0039] As can be seen from Table 2: except that C01 and C02 are controls, and no cluster buds occur due to the influence of the starting medium formula, all other serial numbers (all formulas) can induce cluster buds, but the degree of cluster bud occurrence is quite different , the best o...

Embodiment 3

[0045] Embodiment 3: the rooting cultivation and transplanting of strong seedlings of seedlings:

[0046] 1. Rooting culture of strong seedlings: divide the above-mentioned sprouts into clusters of 2-3 strains and transfer them to strong seedlings or rooting medium for strong seedlings, see Figure 4 , cultivated under 2000-2500LX light for 14h / d, the small sprouts can 100% grow into normal strong plants with multiple roots, stems and leaves in about 28 days. According to the situation of the sprouts, the ones with better growth conditions are directly transferred to the strong seedling medium for cultivation, and the weaker ones are first cultivated in the strong seedling rooting medium for several days, and then moved into the strong seedling medium for cultivation, the strong seedling medium The formula is: Ms+6-BA0.5mg / L+IBA0.2mg / L; the formula of strong seedling rooting medium is: Ms+6-BA0.1mg / L+IBA0.5mg / L. When the concentration of 6-BA in the medium is 0.5mg / L and the ...

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Abstract

The invention discloses a method for rapid propagation of seedlings through tillandsia lateral bud tissue culture. The method comprises the following steps that 1) tillandsia lateral buds are picked;2) the lateral buds are disinfected, sterilized and cultured; 3) cluster buds are induced and cultured; 4) rapid propagation and differentiation culture of bud seedlings are conducted; 5) strong seedlings are subjected to rooting culture; 7) seedling hardening and transplanting are conducted on aseptic seedlings. The method has the advantages that the operation is simple, materials are convenientto obtain, the propagation coefficient is significantly higher than that in the outdoor, and the growth speed of the seedlings is high. By means of rapid propagation culture in this way, each lateralbud can be averagely proliferated into about 11 buds in about 120 days, the propagation coefficient is obviously higher than that in the outdoor, the seedlings are high in growth speed and very strong, a large quantity of tissue culture seedlings which are consistent in property, consistent in growth and strong can be rapidly propagated within a short time, the aim of rapid propagation is achieved, the tissue culture seedlings can easily survive after being transplanted outdoors through seedling hardening, and the survival rate is up to 100%.

Description

technical field [0001] The invention relates to the field of plant breeding, in particular to a method for rapidly propagating seedlings through tissue culture of lateral buds of air pineapple. Background technique [0002] Air pineapple is a kind of root-reduced plant that does not need to be planted in the soil, does not need roots to absorb water and fertilizer, but only absorbs water and nutrients in the atmosphere through its leaves, and can satisfy its own growth and development. It has a wide variety of plant types and shapes, in various poses and with different expressions, colorful flowers and leaves, and is very individual and beautiful. Air pineapple not only has the advantages of strong ornamental value and good decorative effect, but also has the ability to absorb formaldehyde, styrene, CO 2 And other powerful air purification function. Because of these characteristics, it is more and more favored by people as an ornamental plant, and its potential business op...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 梁慧敏李成龙梁栋刘艳张文博韩鹏夏阳郭正兵
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY