Method for evolution and domestication of halophilic microalgae

A technology of microalgae and salina, applied in the direction of using microorganisms, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of large environmental impact, difficult source of raw materials, low yield, etc., to increase production, It is beneficial to environmental protection and the effect of improving the level of lutein produced by salina

Inactive Publication Date: 2019-12-17
杭州江淮环动科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the production of lutein mostly uses higher plants (such as marigolds, etc.) as raw materials, and there are problems such as difficult source of raw materials, low yield, and great impact on the environment; while the production of lutein by microalgae is currently relatively low. , and use high-salt medium, the cost is higher

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  • Method for evolution and domestication of halophilic microalgae
  • Method for evolution and domestication of halophilic microalgae
  • Method for evolution and domestication of halophilic microalgae

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Embodiment 1

[0019] Such as figure 1 Shown is the basic flow of the implementation of the adaptive evolution method, a method for the evolution and domestication of halophilic microalgae, which specifically includes the following steps:

[0020] 1) Streak Dunaliella salina (University of Texas Culture Collection, No. UTEX LB#200) on the Gg-8 plate medium and place it at 20 μE / m 2 / s under the condition of fluorescent light, the culture temperature was 20°C, and the culture time was 2 weeks, and isolated Salina algae colonies were obtained.

[0021] 2) Inoculate the colony of Salina algae obtained in step 1 into a shake flask containing 30 ml of Gg-8 medium at a rotation speed of 100 rpm, and culture at 20° C. for 5 days to obtain activated algal species.

[0022] 3) Inoculate the activated algae species obtained in step 2 into a photobioreactor containing 300ml Gg-8 medium, the volume of the activated algae species inoculated accounts for 20% of the medium, and the volume concentration is...

Embodiment 2

[0029] A method for evolution and domestication of halophilic microalgae, specifically comprising the steps of:

[0030] 1) Streak Dunaliella salina (University of Texas Culture Collection Center No. UTEX LB#1644) on the Gg-8 plate medium and place it at 50 μE / m 2 / s under the condition of fluorescent light, the culture temperature was 25°C, and the culture time was 1 week, and isolated Salina algae colonies were obtained.

[0031] 2) Inoculate the colony of Salina salina obtained in step 1 into a shake flask containing 50 ml of Gg-8 medium at a rotation speed of 150 rpm, and culture at 25° C. for 3 days to obtain activated algal species.

[0032] 3) Inoculate the activated algae species obtained in step 2 into a photobioreactor containing 400ml Gg-8 medium, the volume of the activated algae species inoculated accounts for 25% of the medium, and the volume concentration is 2.0% carbon dioxide, The pH of the control medium was 7.5, the culture temperature was 25°C, and the light...

Embodiment 3

[0039] A method for evolution and domestication of halophilic microalgae, specifically comprising the steps of:

[0040] 1) Streak Dunaliella pasteurii (University of Texas Culture Collection, No. UTEX LB#2538) on the Gg-8 plate medium, and place at 50 μE / m 2 / s under the condition of fluorescent light, the culture temperature is 20°C, and the culture time is about 2 weeks, and isolated Salina algae colonies are obtained.

[0041] 2) Inoculate the colony of Salina salina obtained in step 1 into a shaking flask containing 50 ml of Gg-8 medium at a rotation speed of 100 rpm, and culture at 20° C. for 5 days to obtain activated algal species.

[0042] 3) Inoculate the activated algae species obtained in step 2 into a photobioreactor containing 300ml Gg-8 medium, the volume of the activated algae species inoculated accounts for 25% of the medium, and the volume concentration is 1% carbon dioxide, The pH of the control medium was 6.5, the culture temperature was 25°C, and the ligh...

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Abstract

The invention discloses a method for evolution and domestication of halophilic microalgae, and belongs to the field of biomass resource development and utilization. The method for evolution and domestication of the halophilic microalgae comprises the steps that the halophilic microalgae is separated into salt algae colonies, and then activation is carried out; a multi-step adaptive evolution method is used for reducing cell loss of the halophilic microalgae caused by sudden change in osmotic pressure, and repeated domestication is carried out on the microalgae by a LED photobioreactor. According to the method for evolution and domestication of the halophilic microalgae, the salinity setting is adjusted and controlled, the environmental pressure is changed reasonably, and the adaptive evolution of the halophilic microalgae is achieved, so that the lutein production under seawater-like salinity culture conditions is improved. The method for evolution and domestication of the halophilic microalgae has the characteristics of environmental friendliness and is beneficial to the utilization of marine resources and environmental protection, adaptive evolution and domestication of the halophilic microalgae driven by environment stress is carried out by gradually reducing the salinity, so that the level of lutein produced by salt algae is significantly improved, and the good industrial prospect is achieved.

Description

technical field [0001] The invention belongs to the field of utilization of biomass resources, and in particular relates to a method for evolution and domestication of halophilic microalgae. Background technique [0002] Carotenoids, especially pigments such as lutein and beta-carotene, have multi-billion dollar applications worldwide. At present, the demand for natural carotenoids in the global market is increasing. As an antioxidant, lutein has been widely used in the fields of medicine, food and cosmetics. Halophilic Dunaliella salina tends to accumulate a large amount of carotenoids in response to environmental stress, and has been developed as a beta-carotene-producing algal species. In addition to light conditions, salinity is also a very important environmental factor affecting the growth and pigment accumulation of Dunaliella salina. Dunaliella salina often uses high salinity conditions such as 1.5M NaCl salinity as the optimum growth conditions. As a control, the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/36C12N1/12C12R1/89
CPCC12N1/12C12N1/36
Inventor 傅维琦斯格柔·布瑞诺森
Owner 杭州江淮环动科技有限公司
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