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Virus detection sensor, and device and method for detecting virus concentration

A virus detection and sensor technology, which is applied in the field of virus detection sensors and virus concentration detection devices, can solve the problems of cumbersome implementation process, increased inspection cost, high technical requirements, etc., and achieve simplification of cumbersome operation process, low cost and simplified process flow Effect

Active Publication Date: 2019-12-17
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the implementation process of the above devices is too cumbersome, the technical requirements are high, the efficiency is not high, the cost is high, and the inspection cost is increased, which is not conducive to popularization

Method used

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  • Virus detection sensor, and device and method for detecting virus concentration
  • Virus detection sensor, and device and method for detecting virus concentration
  • Virus detection sensor, and device and method for detecting virus concentration

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The structure of the virus detection sensor provided in this embodiment is as follows: figure 1 As shown, including stand, detection probe and capillary glass tube 1.

[0055] The bracket includes a support plate and a clamp, and there are two clamps. The fixture includes a support body 4 and an elastic C-shaped clamp 3. Both the support body 4 and the support plate 5 are square. The support body 4 is symmetrically fixed on one surface of the support plate 5. One side of the two support bodies 4 is respectively connected The boards are close to each other, and there is a symmetrical semicircular third groove 10 on the other side of the two supporting bodies 4 which is parallel to the supporting board 5 and not in contact with it.

[0056] The detection probe includes a detection body 2 and a photoelectric conversion circuit. The photoelectric conversion circuit includes a photoresistor 9 and a light emitting diode 8 . The detection body 2 is square, the detection bod...

Embodiment 2

[0073] The structure of the device for detecting virus concentration provided by this embodiment is as follows: Figure 4 As shown, it includes the virus detection sensor, power mechanism, valve control mechanism, reagent supply mechanism, magnetic separation mechanism, detection mechanism, first waste liquid bottle 55 and second waste liquid bottle 56 of Embodiment 1.

[0074] The power mechanism includes an air suction pump 11, a first air extraction branch pipe 12, a first valve 13, a second air extraction branch pipe 26 and a second valve 27, and both the first valve 13 and the second valve 27 are electric proportional valves. One end of the first air extraction branch pipe 12 and the second air extraction branch pipe 26 is connected with the outlet of the air pump 11, the first valve 13 is installed on the first air extraction branch pipe 12, and the second valve 27 is installed on the second air extraction branch pipe 26 .

[0075] Reagent supply mechanism comprises the...

Embodiment 3

[0085] 1. Preparation of antibody-modified magnetic sphere suspension:

[0086] 1) Place the EP tube in a Mettler AT20 microbalance and set it to zero, and weigh 4 mg of 10 μg / ml of EDC and 2 mg of 5 μg / ml of NHS with the EP tube;

[0087] 2) Take 20 μL of magnetic nanospheres into the EP tube, then inject PBS buffer solution (PH=6.8) with a pipette gun, rotate and wash on the magnetic stand, and suck out the PBS solution with a pipette gun, and clean the magnetic nanospheres according to this method Three times, after washing, add 400 μL of PBS solution to the EP tube;

[0088] 3) Add EDC and NHS to the EP tube. EDC is insoluble, so add it first, and then place the EP tube in a constant temperature shaker for 30 minutes;

[0089] 4) After shaking, wash three times with PBS buffer solution (PH=7.2) on the magnetic stand to remove excess EDC and NHS powder;

[0090] 5) Add the H7N9 antibody to the EP tube, shake it in a constant temperature shaker for 4 hours, and obtain the ...

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Abstract

The invention discloses a virus detection sensor, and a device and a method for detecting virus concentration. The sensor comprises a bracket, a detection probe and a capillary glass tube. The inner wall of the capillary glass tube is modified with an antibody specifically bound to a virus to be detected. The detection probe comprises a detection body and a photoelectric conversion circuit. The photoelectric conversion circuit comprises a photosensitive element and a light source. The detection body is fixed on the bracket, and is provided with a circular detection channel in the center. The capillary glass tube penetrates through the detection channel, and is fixed on the bracket. A first groove and a second groove are symmetrically formed in the side wall of the detection channel. The light source is fixed in the first groove, and the photosensitive element is fixed in the second groove. The sensor is simple in structure and ingenious in design. The shading performance of a magneticball and photoelectric conversion are ingeniously combined, and detection is convenient and rapid. The device is simple in structure, can realize automatic detection of viruses, and improves the efficiency and precision of detection. The method is simple, uses a standard curve method for detection, and is convenient and fast.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a virus detection sensor, a device and a method for detecting virus concentration. Background technique [0002] At present, there are many kinds of human diseases caused by viruses, and different viruses have different pathogenic mechanisms. At the cellular level, the main destructive effect of viruses is to cause cell lysis, thereby causing cell death. In multicellular organisms, once enough cells in the body die, there are consequences for the health of the organism. Some viruses are capable of causing chronic infection, replicating continuously in the body independent of host defense systems. People who are chronically infected are carriers because they have a reservoir of virus that remains infectious, and when there is a high proportion of carriers in the population, the disease can develop into an epidemic. Therefore, virus detection technology is of great s...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/56983G01N33/54326Y02A50/30
Inventor 洪少力秦莉张泽芬张琴韵汤曼张南刚刘侃
Owner WUHAN TEXTILE UNIV
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