Virus detection sensor, and device and method for detecting virus concentration

A virus detection and sensor technology, which is applied in the field of virus detection sensors and virus concentration detection devices, can solve the problems of cumbersome implementation process, increased inspection cost, high technical requirements, etc., and achieve simplification of cumbersome operation process, low cost and simplified process flow Effect

Active Publication Date: 2019-12-17
WUHAN TEXTILE UNIV
View PDF10 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the implementation process of the above devices is too cumbersome, the technical requirements are high, the e

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Virus detection sensor, and device and method for detecting virus concentration
  • Virus detection sensor, and device and method for detecting virus concentration
  • Virus detection sensor, and device and method for detecting virus concentration

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0053] Example 1

[0054] The structure of the virus detection sensor provided in this embodiment is as follows: figure 1 As shown, it includes holder, detection probe and capillary glass tube 1.

[0055] The bracket includes a support plate and a clamp, and the clamp has two. The fixture includes a support body 4 and an elastic C-shaped clamp 3. Both the support body 4 and the support plate 5 are square. The support body 4 is symmetrically fixed on one surface of the support plate 5. The plates are in close contact with each other, and a semicircular third groove 10 is symmetrically arranged on the other side of the two support bodies 4 that is parallel to and not in contact with the support plate 5 .

[0056] The detection probe includes a detection body 2 and a photoelectric conversion circuit. The photoelectric conversion circuit includes a photoresistor 9 and a light emitting diode 8 . The detection body 2 is in a square shape, the detection body 2 is opaque to light,...

Example Embodiment

[0072] Example 2

[0073] The structure of the device for detecting virus concentration provided in this embodiment is as follows: Figure 4 As shown, it includes the virus detection sensor, power mechanism, valve control mechanism, reagent supply mechanism, magnetic separation mechanism, detection mechanism, first waste liquid bottle 55 and second waste liquid bottle 56 of Example 1.

[0074] The power mechanism includes a suction pump 11, a first suction branch pipe 12, a first valve 13, a second suction branch pipe 26 and a second valve 27. The first valve 13 and the second valve 27 are both electrical proportional valves. One end of the first suction branch pipe 12 and the second suction branch pipe 26 are both connected to the outlet of the suction pump 11 , the first valve 13 is installed on the first suction branch pipe 12 , and the second valve 27 is installed on the second suction branch pipe 26 .

[0075] The reagent supply mechanism includes a first reagent bottle...

Example Embodiment

[0084] Example 3

[0085] 1. Preparation of antibody-modified magnetic sphere suspension:

[0086] 1) Place the EP tube in a METTLER TOLEDO AT20 microbalance to zero, and use the EP tube to weigh 4 mg of 10 μg / ml EDC and 2 mg of 5 μg / ml NHS;

[0087] 2) Take 20 μL of magnetic nanospheres into the EP tube, then inject PBS buffer (PH=6.8) with a pipette, spin and wash on the magnetic stand, suck out the PBS solution with a pipette, and wash the magnetic nanospheres according to this method After washing three times, add 400 μL of PBS solution to the EP tube;

[0088] 3) Add EDC and NHS to the EP tube, EDC is relatively insoluble, so it should be added first, and then the EP tube is placed in a constant temperature oscillator to shake for 30 minutes;

[0089] 4) After shaking, wash three times on a magnetic stand with PBS buffer (PH=7.2) to wash off excess EDC and NHS powder;

[0090] 5) Add H7N9 antibody to the EP tube, shake in a constant temperature oscillator for 4 hours t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a virus detection sensor, and a device and a method for detecting virus concentration. The sensor comprises a bracket, a detection probe and a capillary glass tube. The inner wall of the capillary glass tube is modified with an antibody specifically bound to a virus to be detected. The detection probe comprises a detection body and a photoelectric conversion circuit. The photoelectric conversion circuit comprises a photosensitive element and a light source. The detection body is fixed on the bracket, and is provided with a circular detection channel in the center. The capillary glass tube penetrates through the detection channel, and is fixed on the bracket. A first groove and a second groove are symmetrically formed in the side wall of the detection channel. The light source is fixed in the first groove, and the photosensitive element is fixed in the second groove. The sensor is simple in structure and ingenious in design. The shading performance of a magneticball and photoelectric conversion are ingeniously combined, and detection is convenient and rapid. The device is simple in structure, can realize automatic detection of viruses, and improves the efficiency and precision of detection. The method is simple, uses a standard curve method for detection, and is convenient and fast.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a virus detection sensor, a device and a method for detecting virus concentration. Background technique [0002] At present, there are many kinds of human diseases caused by viruses, and different viruses have different pathogenic mechanisms. At the cellular level, the main destructive effect of viruses is to cause cell lysis, thereby causing cell death. In multicellular organisms, once enough cells in the body die, there are consequences for the health of the organism. Some viruses are capable of causing chronic infection, replicating continuously in the body independent of host defense systems. People who are chronically infected are carriers because they have a reservoir of virus that remains infectious, and when there is a high proportion of carriers in the population, the disease can develop into an epidemic. Therefore, virus detection technology is of great s...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/569G01N33/543
CPCG01N33/56983G01N33/54326Y02A50/30
Inventor 洪少力秦莉张泽芬张琴韵汤曼张南刚刘侃
Owner WUHAN TEXTILE UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap