A method for efficiently collecting and purifying germination stimulators of sunflower rowan using aeroponics and solid-phase extraction techniques
An extraction technology and a solid-phase extraction small column technology are applied in the field of efficiently collecting and purifying germination stimuli of sunflower sprouting, which can solve the problems of few types, concentration and purity of germination stimuli in sunflower sprouting, so as to reduce the pretreatment process, High concentration and purity, high concentration effect
Active Publication Date: 2020-07-24
ZHEJIANG UNIV
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Problems solved by technology
[0009] The object of the present invention is to solve the problem of less types, low concentration and low purity of the sunflower radang germination stimulant extracted and purified in the prior art by utilizing the method of efficiently collecting and purifying the sunflower radang germination stimulant by means of aeroponics and solid-phase extraction technology
Method used
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Embodiment 1
[0058] 1. Test method
[0059] (1) Seed preparation and treatment
[0060] Calculate the amount of seeds needed based on the germination rate of the seeds (test the germination rate in advance). In this experiment, 4 sunflower varieties (genotye) were selected, and each variety had 24 replicates. A total of 96 plants were needed, and about 100 sunflower seeds were prepared in advance for each variety.
[0061] (2) Nutrient solution configuration
[0062] The sunflower plant culture medium uses the improved 1 / 2 Hoagland's nutrient solution (half strength Hoagland's solution). The configuration of the modified 1 / 2 Hoagland's solution is shown in the table, and the final pH value is 5.8. The capacity and volume of the nutrient solution depends on the experimental situation. In this experiment, the concentrated solution of the nutrient solution is prepared first, bottled for later use, and then diluted for use. For example, use a plastic bucket with a volume capacity of 25L. ...
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The invention discloses a method for efficiently collecting and purifying sunflower germination stimulants by using aeroponics and solid-phase extraction techniques. The method comprises: orienting sunflower seeds, and after germination, planting sunflower seedlings in an aeroponic device, using aeroponics Systematic cultivation of sunflower seedlings; in the aeroponic stage, the sunflower seedlings are first cultivated with phosphorus-containing aeroponic nutrient solution, and after 20 to 25 days of cultivation, they are replaced with phosphorus-free aeroponic nutrient solution for starvation culture treatment. The time for starvation culture treatment is 5-7 days; pass all the nutrient solution in the aeroponic device through the solid-phase extraction column for solid-phase extraction, and obtain the sunflower rowidang germination stimulant. In the present invention, firstly, the aeroponic system technology is used to quickly cultivate sunflowers, and then combined with solid-phase extraction technology to separate and purify the germination stimulators of sunflower helianthus in the root exudates of sunflowers. High purity.
Description
technical field [0001] The invention relates to the fields of plant cultivation technology and biochemical technology, in particular to a method for efficiently collecting and purifying sunflower rowidang germination stimulants by using aeroponics and solid-phase extraction technologies. Background technique [0002] Sunflower is one of the important oil crops in the world, and also one of the main oil crops in my country. Because of its high economic value, easy management, low cost, high benefit, and strong salt and alkali resistance, it is widely used in Northeast my country and It is widely planted in Northwest China, among which the Inner Mongolia Autonomous Region is the largest sunflower production area in my country, followed by Xinjiang. In recent years, with the increase of sunflower production, the parasitic weed Orobanchecumana has appeared in sunflower production areas, seriously endangering sunflower production, and the control of weeds is urgent. [0003] Para...
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IPC IPC(8): A01N43/12A01G31/00A01G22/00C05G1/00A01P21/00
CPCA01N43/12A01G31/00A01G22/00C05B7/00B01D15/325B01D15/1871B01D15/426B01D15/20A01G7/00B01D11/0492Y02P60/21A01G22/60A01G31/02A01N43/08A01N65/12B01D11/0288B01D11/0292B01D21/262
Inventor 胡露飏周伟军杨翀许玲章娜王建蘇朱金文白全江云晓鹏
Owner ZHEJIANG UNIV