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A kind of preparation method and application of tremella oligosaccharide

A technology for oligosaccharides and Tremella polysaccharides, which is applied in the preparation of oligosaccharides and sugar derivatives, and methods based on microorganisms, etc., can solve problems such as inability to degrade oligosaccharides, and achieve fast preparation speed, pollution-free operation, and good toughness. Effect

Active Publication Date: 2021-09-28
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this invention is to degrade the polysaccharide to the molecular weight of 1-500kDa, it cannot effectively degrade it to the molecular weight range of the oligosaccharide

Method used

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  • A kind of preparation method and application of tremella oligosaccharide
  • A kind of preparation method and application of tremella oligosaccharide
  • A kind of preparation method and application of tremella oligosaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] (1) Weigh an appropriate amount of white fungus polysaccharide, add it into deionized water according to the ratio of material to liquid 1:20, mix it evenly, pour it into a high-speed shear + ultrasonic extraction container for later use, and pour it into it according to the mass volume ratio (g / mL) of 3:250 Add nano-quartz sand particles.

[0077] (2) After raising the temperature of the mixed liquid in the container to 50°C, turn on the ultrasonic (power 500W / L), and shear at a high speed for 15min at a rotational speed of 20000r / min.

[0078] (3) centrifuge (8000r / min, 20min), keep the supernatant, and spray dry to obtain the oligosaccharide solid. The average molecular weight is 110000Da, and the yield is 10.16%.

[0079] (4) To investigate the in vitro proliferation effect of tremella oligosaccharides as a carbon source on intestinal probiotics by measuring its absorbance. The probiotic effect is poor.

Embodiment 2

[0081] (1) Weigh an appropriate amount of white fungus polysaccharide, add it into deionized water according to the ratio of material to liquid 1:20, mix it evenly, pour it into a high-speed shear + ultrasonic extraction container for later use, and pour it into it according to the mass volume ratio (g / mL) of 3:250 Add nano-quartz sand particles.

[0082] (2) After raising the temperature of the mixed liquid in the container to 50°C, turn on the ultrasonic (power 500W / L), and shear at a high speed for 20min at a rotational speed of 22000r / min.

[0083] (3) centrifuge (8000r / min, 20min), keep the supernatant, and spray dry to obtain the oligosaccharide solid. The average molecular weight is 48000Da, and the yield is 21.56%.

[0084] (4) To investigate the in vitro proliferation effect of tremella oligosaccharides as a carbon source on intestinal probiotics by measuring its absorbance. The probiotic effect is poor.

Embodiment 3

[0086] (1) Weigh an appropriate amount of white fungus polysaccharide, add it into deionized water according to the ratio of material to liquid 1:20, mix it evenly, pour it into a high-speed shear + ultrasonic extraction container for later use, and pour it into it according to the mass volume ratio (g / mL) of 5:250 Add nano-quartz sand particles.

[0087] (2) After raising the temperature of the mixed liquid in the container to 50°C, turn on the ultrasonic (power 500W / L), and shear at a high speed for 23min at a rotational speed of 24000r / min.

[0088] (3) centrifuge (8000r / min, 20min), keep the supernatant, and spray dry to obtain the oligosaccharide solid. The average molecular weight is 18000Da, and the yield is 28.56%.

[0089] (4) To investigate the in vitro proliferation effect of tremella oligosaccharides as a carbon source on intestinal probiotics by measuring its absorbance. The probiotic effect is poor.

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Abstract

The invention discloses a preparation method and application of tremella oligosaccharides. Tremella polysaccharides are weighed, added to deionized water, mixed evenly, poured into a high-speed shearing and ultrasonic extraction container for standby, and nano-quartz sand particles are added therein. After heating up the mixed liquid in the container, turn on the ultrasonic wave for high-speed shearing; after centrifugation, keep the supernatant and spray dry it to obtain tremella oligosaccharides, which can be used as a carbon source for intestinal probiotics . The present invention combines mechanical force and mechanical waves and adds nano-scale materials to assist, and uses physical methods to directly degrade polysaccharides, avoiding the influence of residual biological reagents and chemical reagents on sugar activity, and does not need to add reaction reagents in the whole process, which is green and environmentally friendly. , not to pollute the environment.

Description

technical field [0001] The invention relates to a preparation method and application of tremella oligosaccharides, in particular to a method for preparing oligosaccharides by degrading tremella polysaccharides in combination with a physical method and its prebiotic effect on intestinal probiotics. Background technique [0002] Tremella, also known as white fungus, white fungus, white fungus, etc., is the fruiting body of the Basidiomycetes Tremellaceae plant. Tremella fruit body is pure white to milky white. Tremella can not only enhance the body's anti-tumor immunity, but also has the functions of nourishing and promoting body fluid, nourishing lungs and stomach, nourishing lungs and Qi. Which is rich in carbohydrates, protein and a variety of amino acids and other ingredients. Tremella, as a nutritious and high-yielding edible fungus, contains about 65%-71.2% carbohydrates and is a good source of oligosaccharides. [0003] Oligosaccharides, also known as oligosaccharides...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00C07H1/00C07H3/06C12N1/20C12N1/38C12R1/23C12R1/01C12R1/225
CPCC07H1/00C07H3/06C08B37/006C12N1/20C12N1/38
Inventor 何荣军刘高丹孙培龙
Owner ZHEJIANG UNIV OF TECH