Medium used for culturing primary cells of solid tumor of colorectal cancer
A technology for colorectal cancer and primary cells, applied in the biological field, can solve the problems of difficult to remove miscellaneous cells, long culture period, low culture success rate, etc.
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Embodiment 1
[0062] Example 1. Preparation of reagents for culturing primary colorectal cancer cells
[0063] 1. Sample preservation solution (100mL)
[0064] The specific formulation of the sample preservation solution (100mL) is shown in Table 1.
[0065] Table 1 Sample Preservation Solution (100mL)
[0066]
[0067] After the sample preservation solution is prepared, it is divided into 15mL centrifuge tubes, 5mL per tube. After aliquoting, it can be stored at 4°C for 1 month.
[0068] 2. Sample cleaning solution (100mL)
[0069] The specific formula of the sample cleaning solution (100mL) is shown in Table 2.
[0070] Table 2 Sample cleaning solution (100mL)
[0071]
[0072] The sample cleaning solution should be prepared and used immediately.
[0073] 3. Sample dissociation solution (10mL)
[0074] The specific formulation of the sample dissociation solution (10mL) is shown in Table 3.
[0075] Table 3 Sample Dissociation Solution (10mL)
[0076]
[0077] Note: The s...
Embodiment 2
[0157] Example 2. Obtaining of postoperative specimens of colorectal cancer
[0158] 1. Cooperate with tertiary hospitals, and the cooperation has passed the formal medical ethics review.
[0159] 2. The attending doctor selects the patients according to the clinical indications stipulated in the medical guidelines, and selects appropriate samples for in vitro culture according to the clinical indications during the operation. The selection criteria for the samples are: primary colorectal cancer, and the pathological stage is Stage II, III or IV, various pathological types of colorectal cancer or metastatic lesions of colorectal cancer, and colorectal cancer surgical specimens weighing more than 20 mg.
[0160] 3. The attending doctor provides basic clinical information such as the patient's gender, age, medical history, family history, smoking history, pathological staging and typing, and clinical diagnosis. The patient's name, ID number and other information related to the ...
Embodiment 3
[0162] Embodiment 3, pre-dissociation treatment of colorectal cancer tissue samples
[0163] The following operations need to be performed on ice, and the entire operation steps need to be completed within 10 minutes.
[0164] The surgical equipment used in the following operations must be sterilized by high temperature and high pressure before use.
[0165] 1. Sample weighing.
[0166] 2. Clean the surface of the sample with 75% (volume percent) ethanol for 10 to 30 seconds.
[0167] 3. Wash the sample 10 times with sample cleaning solution and 5 times with sterile PBS solution.
[0168] 4. Use ophthalmic scissors, ophthalmic forceps, scalpel and other equipment to carefully peel off the adipose tissue, connective tissue, and necrotic tissue in the sample.
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