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Method of quickly identifying natural and artificially synthetic vitamin E in health care product

A technology of artificial synthesis and health products, applied in the field of isomer identification, can solve the problems of inability to identify α-tocopherol, etc., and achieve the effects of easy promotion and use, good separation effect, and shortened identification time

Inactive Publication Date: 2019-12-20
ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for quickly identifying natural and artificially synthesized vitamin E in health products, so as to solve the problem that the traditional method mentioned in the above background technology cannot identify whether α-tocopherol is naturally occurring RRR-α-type or artificially synthesized The DL-alpha-type problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1 (natural vitamin E soft capsule)

[0038] Take 20 capsule samples, squeeze out the contents, mix well, accurately weigh 0.1g (accurate to 0.001 g) of the sample into a 15 mL centrifuge tube, add about 2 mL of warm water, vortex for 30 seconds, mix well, add 3 mL of potassium hydroxide-ethanol saponification solution, vortex for 30 seconds, mix well, shake saponification in a constant temperature water bath at 80 ℃ for 10 min, and immediately cool to room temperature with cold water after saponification;

[0039] Add 10mL of isooctane, vortex for 3 minutes, stand still for 3 minutes, take 1mL of the upper layer extract into a 2mL centrifuge tube filled with 1mL of deionized water, vortex for 30 seconds, centrifuge at 10000 r / min for 3min, and take the supernatant Sample injection was performed according to the method described above.

[0040] According to the measured peak areas of RRR-α-tocopherol and other forms of α-tocopherol, the concentration of RRR-α...

Embodiment 2

[0041] Example 2 (Vitamin E Chewable Tablets)

[0042] Take 20 chewable tablet samples, grind them into powder, and mix them evenly. Accurately weigh 0.1 g (accurate to 0.001 g) of the sample into a 15 mL centrifuge tube, add about 2 mL of warm water, vortex for 30 seconds, mix well, add 3 mL potassium hydroxide-ethanol saponification solution, vortex for 30 seconds, mix Shake saponification in a constant temperature water bath at 80°C for 10 minutes after homogenization, and immediately cool to room temperature with cold water after saponification.

[0043] Add 10mL of isooctane, vortex for 3 minutes, stand still for 3 minutes, take 1mL of the upper layer extract into a 2mL centrifuge tube filled with 1mL of deionized water, vortex for 30 seconds, centrifuge at 10000 r / min for 3min, and take the supernatant Sample injection was performed according to the method described above.

[0044] According to the measured peak areas of RRR-α-tocopherol and other forms of α-tocopherol, ...

Embodiment 3

[0045] Embodiment 3 (vitamin E soft capsule)

[0046] Take 20 capsule samples, squeeze out the contents, and mix well. Accurately weigh 0.1 g (accurate to 0.001 g) of the sample into a 15 mL centrifuge tube, add about 2 mL of warm water, vortex for 30 seconds, mix well, add 3 mL potassium hydroxide-ethanol saponification solution, vortex for 30 seconds, mix Shake saponification in a constant temperature water bath at 80 °C for 10 min after homogenization, and immediately cool to room temperature with cold water after saponification.

[0047] Add 10mL of isooctane, vortex for 3 minutes, stand still for 3 minutes, take 1mL of the upper layer extract into a 2mL centrifuge tube filled with 1mL of deionized water, vortex for 30 seconds, centrifuge at 10000 r / min for 3min, and take the supernatant Injection and determination according to the aforementioned method

[0048] According to the measured peak area of ​​RRR-α-tocopherol and other forms of α-tocopherol, the concentration o...

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PUM

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Abstract

The invention discloses a method of quickly identifying natural and artificially synthetic vitamin E in a health care product. The method comprises the steps of S1, sampling, wherein a to-be-identified sample is taken and prepared into a sample solution; S2, extraction, wherein a saponifying agent is added into the sample solution for a saponification reaction, and an extracting agent is adopted to extract and separate alpha-tocopherol after the saponification reaction; and S3, identification, wherein a high-performance liquid chromatograph is adopted to perform separate identification on extract liquor, and a chromatographic column adopted in the high-performance liquid chromatograph is spherical silica gel with a surface coated with chiral polymer cellulose-tri(3,5)dimethyl phenyl carbamate. According to the method, RRR-alpha-tocopherol and alpha-tocopherol in other forms in the sample are chirally split by use of the chiral chromatographic column, the separation degree is greater than 1.5, and the separation effect is good; besides, the extract liquor is directly used as the sample for analysis through the high-performance liquid chromatograph, the whole detection process can becompleted within 30 minutes, the identification time is greatly shortened, and the working efficiency is improved; and the method is simple in operation process and convenient to use and popularize.

Description

technical field [0001] The invention relates to the related technical field of isomer identification, in particular to a method for quickly identifying natural and synthetic vitamin E in health care products. Background technique [0002] Vitamin E is a type of fat-soluble vitamin, which has the functions of anti-aging, improving immunity, and auxiliary treatment of cardiovascular and cerebrovascular diseases. It is widely used in food, medicine, health care products, cosmetics and other fields. The products include natural and synthetic type. Naturally occurring vitamin E contains 4 types of α, β, γ, and δ tocopherols and 4 types of tocotrienols. Their structural differences lie in the number of methyl groups on the benzene ring and the unsaturated double bonds on the aliphatic chain. Naturally occurring All α-tocopherols are D-type or RRR-type (that is, all three chiral carbons are in R-type), which is dextrorotatory and is a single isomer. The artificially synthesized v...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/74
CPCG01N30/02G01N30/06G01N30/74G01N2030/062
Inventor 黄百芬张京顺许娇娇
Owner ZHEJIANG CENT FOR DISEASE CONTROL & PREVENTION