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Culture medium group for tissue culture rapid propagation of dracocephalum plants and application thereof

A tissue culture rapid propagation and culture medium technology, which is applied in the fields of application, plant regeneration, botany equipment and methods, etc., can solve the problems of tissue material browning, high seed reproduction efficiency, and difficulty in collection, so as to promote bud rooting, The effect of efficiently inducing callus and effectively inducing bud formation and growth

Active Publication Date: 2019-12-27
成都及禾生物科技有限公司
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0017] However, at present, when using the phytohormone ratio in the conventional plant tissue culture medium to breed the plants of the genus Cymbidium, the efficiency is very low, even not as high as the seed propagation efficiency
In addition, due to the small size of Maojiancao seeds, it is not easy to collect, and the seed germination rate is not high in the natural environment, which makes it difficult to reproduce the seeds; at the same time, because of its rich secondary metabolites, it contains a variety of enzymes and various small Molecular substances have seriously affected the effect of the phytohormone combination of conventional plant tissue culture, so that the conventional plant tissue culture method can not effectively achieve the efficiency of tissue culture for the breeding of Maojiancao
For example, the commonly used media for tobacco tissue culture cannot complete the regeneration of plants of the genus Pseudomonas genus, such as Maojiancao, Minshan Maojiancao, and Songye Qinglan. The induction medium cannot induce enough callus tissue. During the induction process, the tissue The browning of the material is also obvious

Method used

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  • Culture medium group for tissue culture rapid propagation of dracocephalum plants and application thereof
  • Culture medium group for tissue culture rapid propagation of dracocephalum plants and application thereof
  • Culture medium group for tissue culture rapid propagation of dracocephalum plants and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. a culture medium group of the tissue culture rapid propagation of blue orchid plant, comprise induction medium, differentiation medium and rooting medium.

[0042] Among them, the induction medium includes 0.8MS medium, 6-BA 0.5mg / L, IAA 0.1mg / L, 2,4-D0.1mg / L, sucrose 30g / L, agar 5g / L, Vc 0.2mg / L , Sodium nitrosoferricyanide 2.0mg / L, pH=5.6;

[0043] Differentiation medium includes 1.5MS medium, 6-BA 0.8mg / L, NAA 0.1mg / L, IAA 0.1mg / L, sucrose 30g / L, agar 5g / L, pH=6.2;

[0044] Rooting medium includes 0.8MS medium, NAA 0.15mg / L, IBA 0.025mg / L, sucrose 20g / L, agar 5g / L, potato juice 2.0mL / L, activated carbon 1g / L, pH=5.8.

[0045] 2. The application method of the culture medium group of the tissue culture rapid propagation of a kind of blue orchid

[0046] Among them, the induction medium is used to induce culture of the explants of Maojiancao to obtain callus (the callus rate is 86.85%); the differentiation medium is used to differentiate and cultivate the callus to...

Embodiment 2

[0048] 1. a culture medium group of the tissue culture rapid propagation of blue orchid plant, comprise induction medium, differentiation medium and rooting medium.

[0049] Among them, the induction medium includes 1.5MS medium, 6-BA 1.0mg / L, IAA 0.3mg / L, 2,4-D 0.2mg / L, sucrose 30g / L, agar 5g / L, Vc 0.4mg / L , Sodium nitroferricyanide 6.0mg / L, pH=6.2;

[0050] Differentiation medium includes 0.8MS medium, 6-BA 0.5mg / L, NAA 0.05mg / L, IAA 0.05mg / L, sucrose 30g / L, agar 5g / L, pH=5.6;

[0051] Rooting medium includes 1.0MS medium, NAA 0.2mg / L, IBA 0.05mg / L, sucrose 25g / L, agar 5g / L, potato juice 3.0mL / L, activated carbon 1g / L, pH=6.2.

[0052] 2. The application method of the culture medium group of the tissue culture rapid propagation of a kind of blue orchid

[0053] Among them, the induction medium is used to induce the explants of Maojiancao to obtain callus (the callus rate is 94.48%); the differentiation medium is used to differentiate and cultivate the callus to obtain adve...

Embodiment 3

[0055] 1. a culture medium group of the tissue culture rapid propagation of blue orchid plant, comprise induction medium, differentiation medium and rooting medium.

[0056] Among them, the induction medium includes MS medium, 6-BA 0.8mg / L, IAA 0.2mg / L, 2,4-D 0.15mg / L, sucrose 30g / L, agar 5g / L, Vc 0.3mg / L, Sodium nitrosoferricyanide 4.0mg / L, pH=5.8;

[0057] Differentiation medium includes MS medium, 6-BA 0.7mg / L, NAA 0.08mg / L, IAA 0.08mg / L, sucrose 30g / L, agar 5g / L, pH=5.8;

[0058] Rooting medium includes 0.5MS medium, NAA 0.1mg / L, sucrose 15g / L, agar 5g / L, potato juice 1.0mL / L, activated carbon 1g / L, pH=5.6.

[0059] 2. The application method of the culture medium group of the tissue culture rapid propagation of a kind of blue orchid

[0060] Wherein, the induction medium is used for inducing culture to the explant of Mao Jiancao, to obtain callus (the callus rate is 88.15%); Differentiation medium is used for the differentiation culture of callus, to obtain adventitious ...

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Abstract

The invention relates to the technical field of plant cell induction and tissue culture and discloses a culture medium group for tissue culture rapid propagation of dracocephalum plants. The culture medium group comprises an induction culture medium, a differentiation culture medium and a rooting culture medium, the induction culture medium comprises 0.8-1.5MS culture medium, 0.5-1.0mg / L of6-BA, 0.1-0.3mg / L of IAA, 0.1-0.2mg / L of 2, 4-D, 0.2-0.4mg / L of Vc, 2.0-6.0mg / L of sodium nitroprusside, 25-30g / L of sucrose and 5-7g / L of agar, and pH of the induction culture medium is 5.6-6.2; the differentiation culture medium comprises 0.8-1.5MS culture medium, 0.5-1mg / L of 6-BA, 0.05-0.1mg / L of NAA, 0.05-0.1mg / L of IAA, 25-30g / L of sucrose and 5-7g / L of agar, and pH of the differentiation culture medium is 5.6-6.2; the rooting culture medium comprises a 0.5-1.0MS culture medium, 0.1-0.2mg / L of NAA, 0-0.05mg / L of IBA, 15-25g / L of sucrose, 5-7g / L of agar and 1.0-3.0mL / L of potato juice. The invention further discloses application of the culture medium group. Through targeted limiting of culture medium composition and proportion of multiple plant hormone combination, effect of efficient propagation of cultivation seedlings of the dracocephalum plants is realized.

Description

technical field [0001] The invention relates to the technical field of plant cell induction and tissue culture, in particular to a culture medium group for tissue culture and rapid propagation of plants of the genus Cymbidium and its application. Background technique [0002] Dracocephalum belongs to Labiatae, herbaceous, perennial, with woody rhizomes, and rarely annual. Stems often arise from rhizomes, erect, sparsely spread, often unbranched or with few branches, sparsely numerous branches, quadrangular. Leaves opposite, basal leaves with long stalks, cauline leaves with short stalks or sessiles, often heart-shaped ovate or oblong, or lanceolate, margins crenate or serrate, or entire, usually not Divided or pinnately nearly palmately parted. Verticels densely clustered into heads or spikes or sparsely arranged; flowers usually blue-purple, rarely white; bracts often obovate, often with sharp teeth or spines, rarely entire. The phytochemical composition of the genus Qin...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 秦小波
Owner 成都及禾生物科技有限公司
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