Culture medium group for tissue culture rapid propagation of dracocephalum plants and application thereof

A tissue culture rapid propagation and culture medium technology, which is applied in the fields of application, plant regeneration, botany equipment and methods, etc., can solve the problems of tissue material browning, high seed reproduction efficiency, and difficulty in collection, so as to promote bud rooting, The effect of efficiently inducing callus and effectively inducing bud formation and growth
CN110612904AActive Publication Date: 2019-12-27成都及禾生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
成都及禾生物科技有限公司
Publication Date
2019-12-27

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Abstract

The invention relates to the technical field of plant cell induction and tissue culture and discloses a culture medium group for tissue culture rapid propagation of dracocephalum plants. The culture medium group comprises an induction culture medium, a differentiation culture medium and a rooting culture medium, the induction culture medium comprises 0.8-1.5MS culture medium, 0.5-1.0mg / L of6-BA, 0.1-0.3mg / L of IAA, 0.1-0.2mg / L of 2, 4-D, 0.2-0.4mg / L of Vc, 2.0-6.0mg / L of sodium nitroprusside, 25-30g / L of sucrose and 5-7g / L of agar, and pH of the induction culture medium is 5.6-6.2; the differentiation culture medium comprises 0.8-1.5MS culture medium, 0.5-1mg / L of 6-BA, 0.05-0.1mg / L of NAA, 0.05-0.1mg / L of IAA, 25-30g / L of sucrose and 5-7g / L of agar, and pH of the differentiation culture medium is 5.6-6.2; the rooting culture medium comprises a 0.5-1.0MS culture medium, 0.1-0.2mg / L of NAA, 0-0.05mg / L of IBA, 15-25g / L of sucrose, 5-7g / L of agar and 1.0-3.0mL / L of potato juice. The invention further discloses application of the culture medium group. Through targeted limiting of culture medium composition and proportion of multiple plant hormone combination, effect of efficient propagation of cultivation seedlings of the dracocephalum plants is realized.
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Description

technical field

[0001] The invention relates to the technical field of plant cell induction and tissue culture, in particular to a culture medium group for tissue culture and rapid propagation of plants of the genus Cymbidium and its application. Background technique

[0002] Dracocephalum belongs to Labiatae, herbaceous, perennial, with woody rhizomes, and rarely annual. Stems often arise from rhizomes, erect, sparsely spread, often unbranched or with few branches, sparsely numerous branches, quadrangular. Leaves opposite, basal leaves with long stalks, cauline leaves with short stalks or sessiles, often heart-shaped ovate or oblong, or lanceolate, margins crenate or serrate, or entire, usually not Divided or pinnately nearly palmately parted. Verticels densely clustered into heads or spikes or sparsely arranged; flowers usually blue-purple, rarely white; bracts often obovate, often with sharp teeth or spines, rarely entire. The phytochemical composition of the genus Qin...

Claims

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