Glucose dehydrogenase enzyme activity accurate qualitative and quantitative method

A technology of stationary phase and mobile phase, applied in the field of enzyme activity detection, to achieve mild reaction conditions, wide application range, and good stability of the method
CN110618203AInactive Publication Date: 2019-12-27HEC PHARM
7 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HEC PHARM
Publication Date
2019-12-27
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention relates to the technical field of enzyme activity detection, and specifically relates to a glucose dehydrogenase enzyme activity accurate qualitative and quantitative method. According to the method, an HPLC detection method of a product NADPH of a glucose dehydrogenase enzymatic reaction is first provided. In the method, a reversed phase column in which a silane bonded phaseis used as a stationary phase is used, a moving phase consists of buffer salt and an organic solvent, an ultraviolet detect wavelength is 330 to 350 nm, and a flow velocity is 0.8 to 1.2 mL / min, so as to provide the glucose dehydrogenase enzyme activity accurate qualitative and quantitative method. The detection method provided in the present invention is simple in operation, high in sensitivity,strong in accuracy, less vulnerable to insoluble particles and some pigments in a complicated sample, and is easy for standard operation.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of enzyme activity detection, in particular to a method for accurately qualitative and quantitative glucose dehydrogenase enzyme activity. Background technique

[0002] Glucose Dehydrogenase (Glucose Dehydrogenase, E.C.1.1.1.47, referred to as GDH) mainly exists in the liver of animals and Acetobacter suboxidans, and can catalyze β-D-glucose to generate gluconic acid (D-glucono-δ-lactone ), accompanied by the generation of the reducing coenzyme NADPH. As early as 1975, Banauch et al. used glucose dehydrogenase as a diagnostic enzyme for clinical blood glucose determination to measure the concentration of glucose in human body fluids. Currently, glucose dehydrogenase is mainly used in clinical glucose monitoring sensors and NADPH regeneration mediated by NADP-dependent glucose dehydrogenase.

[0003] At present, the main detection methods of glucose dehydrogenase activity include spectrophotometry, ion chro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More